Results 11 to 20 of about 326,708 (370)
Heterogeneous and rate-dependent streptavidin–biotin unbinding revealed by high-speed force spectroscopy and atomistic simulations [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2018 Significance Protein–ligand interactions are commonly described in terms of a two-state or a lock-and-key mechanism. To provide a more detailed and dynamic description of receptor–ligand bonds and their (un)binding path, we combined high-speed force ...
F. Rico +4 more
semanticscholar +6 more sources
Beilstein Journal of Nanotechnology, 2017 Gold nanoparticles (AuNPs) exhibit unique properties that can be modulated through a tailored surface functionalization, enabling their targeted use in biochemical sensing and medical diagnostics.
Roberta D'Agata +2 more
doaj +2 more sources
Improvement of production, assay and purification of streptavidin [PDF]
Journal of Industrial Microbiology, 1990 The production of streptavidin by Streptomyces avidinii in several different media was examined at 24, 48 and 72 hours. Flask studies indicated that fermentation media containing either complex or multiple carbon sources resulted in higher yields of streptavidin than media with a single carbon source.
Robert J. Toso +3 more
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Journal of Proteome Research, 2020 The streptavidin-based enrichment of biotin-tagged molecules is a common methodology that is routinely used across multiple disciplines in biomedical research. Numerous and varied formats of immobilized streptavidin and related proteins are available, but predicting which product is most apt for a given application is complicated by the fact that there
Linda Berg Luecke +2 more
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Hemolytic Activity of pH-Responsive Polymer-Streptavidin Bioconjugates [PDF]
, 1999 Drug delivery systems that increase the rate and/or quantity of drug release to the cytoplasm are needed to enhance cytosolic delivery and to circumvent nonproductive cell trafficking routes. We have previously demonstrated that poly(2-ethylacrylic acid)
Chantal A. Lackey +5 more
openalex +4 more sources
Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay. [PDF]
PLoS ONE, 2016 Enzyme Linked Immunosorbent Assay (ELISA) is the gold standard assay for detecting and identifying biomolecules using antibodies as the probe. Improving ELISA is crucial for detecting disease-causing agents and facilitating diagnosis at the early stages ...
Thangavel Lakshmipriya +2 more
doaj +2 more sources
Journal of Advanced Pharmaceutical Technology & Research, 2022 Nearly 95% of streptavidin which is expressed in Escherichia coli found as an inclusion body. Protein expressed in an inclusion body form requires further steps for the folding process related to its purification.
Toto Subroto +5 more
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Nanomaterials, 2021 The physiochemical properties of nanomaterials have a close relationship with their status in solution. As a result of its better simplicity than that of pre-assembled aggregates, the in situ assembly of nanomaterials has been integrated into the design ...
Yong Chang +4 more
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Effective Tumor Targeting by EphA2-Agonist-Biotin-Streptavidin Conjugates
Molecules, 2021 We recently reported on a potent synthetic agent, 135H11, that selectively targets the receptor tyrosine kinase, EphA2. While 135H11 possesses a relatively high binding affinity for the ligand-binding domain of EphA2 (Kd~130 nM), receptor activation in ...
Parima Udompholkul +6 more
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Cancer Medicine, 2022 Background CARs are engineered receptors comprising an immunoglobulin single‐chain variable fragment (scFv) that identifies and binds to the target antigen, a transmembrane domain, and an intracellular T‐cell signaling domain.
Hui Lian +10 more
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