Results 11 to 20 of about 323,420 (331)
Streptavidin-Hosted Organocatalytic Aldol Addition [PDF]
Molecules, 2020 In this report, the streptavidin-biotin technology was applied to enable organocatalytic aldol addition. By attaching pyrrolidine to the valeric motif of biotin and introducing it to streptavidin (Sav), a protein-based organocatalytic system was created,
Nicolò Santi +2 more
doaj +4 more sources
The Crystal Structure of Monovalent Streptavidin. [PDF]
Scientific Reports, 2016 The strong interaction between streptavidin (SA) and biotin is widely utilized in biotechnological applications. A SA variant, monovalent SA, was developed with a single and high affinity biotin-binding site within the intact tetramer.
Biswas, Sangita +3 more
core +5 more sources
Protease‐resistant streptavidin for interaction proteomics
Molecular Systems Biology, 2020 Streptavidin‐mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners; however, intense streptavidin‐derived peptides impede protein identification by mass spectrometry.
Mahmoud‐Reza Rafiee +6 more
doaj +3 more sources
Sensors, 2020 While label-free multiplex sensor technology enables “mixing and matching” of different capture molecules in principle, in practice this has been rarely (if ever) demonstrated. To fill this gap, we developed protocols for the preparation of mixed aptamer-
Alanna M. Klose, Benjamin L. Miller
doaj +1 more source
Sensors, 2022 The exceptional strength and stability of noncovalent avidin-biotin binding is widely utilized as an effective bioconjugation strategy in various biosensing applications, and neutravidin and streptavidin proteins are two commonly used avidin analogues ...
Tun Naw Sut +4 more
doaj +1 more source
An improved method for surface immobilisation of RNA: application to small Non-Coding RNA - mRNA pairing [PDF]
, 2013 Characterisation of RNA and its intermolecular interactions is increasing in importance as the inventory of known RNA functions continues to expand. RNA-RNA interactions are central to post-transcriptional gene regulation mechanisms in bacteria, and the ...
Butt, Louise +9 more
core +12 more sources
Streptavidin Coverage on Biotinylated Surfaces [PDF]
ACS Applied Materials & Interfaces, 2021 Biosensors and other biological platform technologies require the functionalization of their surface with receptors to enhance affinity and selectivity. Control over the functionalization density is required to tune the platform's properties. Streptavidin (SAv) monolayers are widely used to immobilize biotinylated proteins, receptors, and DNA.
P. H. Erik Hamming, Jurriaan Huskens
openaire +3 more sources
Synthesis and Application of Albumin Nanoparticles Loaded with Prussian Blue Nanozymes
Colloids and Interfaces, 2022 Prussian blue nanozymes exhibit peroxidase-like catalytic activity and are therefore considered a stable and inexpensive alternative to natural peroxidases in the enzyme-linked immunosorbent assay (ELISA).
Pavel Khramtsov +6 more
doaj +1 more source
Applied Biosciences, 2023 Bacterial contamination of water and food sources is still a major source of diseases. Early detection of potential pathogens is key to prevent their spreading and severe health risks.
Filippo Savini +3 more
doaj +1 more source
Postsecretory modifications of streptavidin [PDF]
Biochemical Journal, 1989 Streptavidin, an extracellular biotin-binding protein from Streptomyces avidinii, exhibits a multiplicity in its electrophoretic mobility pattern which depends both upon the conditions for growth of the bacterium and upon the protocol used in the purification of the protein.
E A, Bayer +3 more
openaire +2 more sources