Results 21 to 30 of about 326,708 (370)

An Analysis of the Biotin–(Strept)avidin System in Immunoassays: Interference and Mitigation Strategies

open access: yesCurrent Issues in Molecular Biology, 2023
An immunoassay is an analytical test method in which analyte quantitation is based on signal responses generated as a consequence of an antibody–antigen interaction. They are the method of choice for the measurement of a large panel of diagnostic markers.
Amy H. A. Balzer   +1 more
doaj   +1 more source

Distinct Binding Properties of Neutravidin and Streptavidin Proteins to Biotinylated Supported Lipid Bilayers: Implications for Sensor Functionalization

open access: yesSensors, 2022
The exceptional strength and stability of noncovalent avidin-biotin binding is widely utilized as an effective bioconjugation strategy in various biosensing applications, and neutravidin and streptavidin proteins are two commonly used avidin analogues ...
Tun Naw Sut   +4 more
doaj   +1 more source

Synthesis and Application of Albumin Nanoparticles Loaded with Prussian Blue Nanozymes

open access: yesColloids and Interfaces, 2022
Prussian blue nanozymes exhibit peroxidase-like catalytic activity and are therefore considered a stable and inexpensive alternative to natural peroxidases in the enzyme-linked immunosorbent assay (ELISA).
Pavel Khramtsov   +6 more
doaj   +1 more source

Modular Set of Reagents in Lateral Flow Immunoassay: Application for Antibiotic Neomycin Detection in Honey

open access: yesBiosensors, 2023
A scheme of modular competitive immunochromatography with an analyte-independent test strip and changeable specific immunoreactants has been proposed.
Lyubov V. Barshevskaya   +3 more
doaj   +1 more source

Streptavidin/biotin: Tethering geometry defines unbinding mechanics

open access: yesScience Advances, 2020
Single-molecule force spectroscopy reveals how the mechanical stability of a widely used biomolecular complex varies fourfold. Macromolecules tend to respond to applied forces in many different ways. Chemistry at high shear forces can be intriguing, with
Steffen M. Sedlak   +3 more
semanticscholar   +1 more source

A Simple Biosensor Based on Streptavidin-HRP for the Detection of Bacteria Exploiting HRPs Molecular Surface Properties

open access: yesApplied Biosciences, 2023
Bacterial contamination of water and food sources is still a major source of diseases. Early detection of potential pathogens is key to prevent their spreading and severe health risks.
Filippo Savini   +3 more
doaj   +1 more source

A Stable Biotin-Streptavidin Surface Enables Multiplex, Label-Free Protein Detection by Aptamer and Aptamer-Protein Arrays Using Arrayed Imaging Reflectometry

open access: yesSensors, 2020
While label-free multiplex sensor technology enables “mixing and matching” of different capture molecules in principle, in practice this has been rarely (if ever) demonstrated. To fill this gap, we developed protocols for the preparation of mixed aptamer-
Alanna M. Klose, Benjamin L. Miller
doaj   +1 more source

Cell-Penetrating Streptavidin: A General Tool for Bifunctional Delivery with Spatiotemporal Control, Mediated by Transport Systems Such as Adaptive Benzopolysulfane Networks

open access: yesJournal of the American Chemical Society, 2020
In this report, cell-penetrating streptavidin (CPS) is introduced to exploit the full power of streptavidin–biotin biotechnology in cellular uptake. For this purpose, transporters, here cyclic oligochalcogenides (COCs), are covalently attached to lysines
J. López-Andarias   +9 more
semanticscholar   +1 more source

Artificial Metalloenzymes Based on the Biotin–Streptavidin Technology: Enzymatic Cascades and Directed Evolution

open access: yesAccounts of Chemical Research, 2019
Conspectus Artificial metalloenzymes (ArMs) result from anchoring a metal-containing moiety within a macromolecular scaffold (protein or oligonucleotide).
A. D. Liang   +3 more
semanticscholar   +1 more source

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