Results 21 to 30 of about 323,420 (331)

Pre-hybridisation: an efficient way of suppressing endogenous biotin-binding activity inherent to biotin–streptavidin detection system [PDF]

, 2014
Endogenous biotin or biotinylated protein binding activity is a major drawback to biotin-avidin/streptavidin detection system. The avidin/streptavidin conjugate used to detect the complex of the biotinylated secondary antibody and the primary antibody ...
Andrew Thompsett, Banks, Bayer, Chandler, Chandler, Dakshinamurti, Emma Spikings, Haneji, Lifespan Biosciences Inc., Lombard, Moss, Praul, Raju Ahmed, Shaobo Zhou, Sheffield, Sigma-Aldrich, Taniguchi, Thermo Scientific, Tiantian Zhang, Vaitaitis, Wood, Wood, Yu   +22 more
core   +1 more source

SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY [PDF]

, 2014
Indexación: Web of Science; Scielo.A hydrogen-terminated Si (111) surface was modified to form an aminoterminated monolayer for immobilization of streptavidin.
ARAYA, EYLEEN, ESCOBAR, CARLOS A., GUELL, ALEIX G., KOGAN, MARCELO J., SANZ, FAUSTO   +4 more
core   +2 more sources

Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines [PDF]

, 1996
A powerful and potentially general approach to the targeting and crystallization of proteins on lipid interfaces through coordination of surface histidine residues to lipid-chelated divalent metal ions is presented.
Arnold, Frances H., Chen, Chao-Tsen, Chilkoti, Ashutosh, Frey, Wolfgang, Pack, Daniel W., Schief, William R., Jr., Stayton, Patrick, Vogel, Viola   +7 more
core   +1 more source

Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry [PDF]

, 2010
We developed a bioadhesive coating based on a synthetic peptide-conjugate (AK-cycloRGDfC]) which contains multiples of the arginyl-glycyl-aspartic acid (RGD) amino acid sequence.
Bert Nickel, Emilia Madarasz, Gabor Mezo, Marko, Martin Huth, Mezo, Pfaff, Samira Hertrich, Szokan, Wang   +9 more
core   +3 more sources

Sorting of chromosomes by magnetic separation [PDF]

, 1988
Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension ...
A Oberteufer, B Schw�ger, B Trask, C Cremer, Christoph Cremer, CS Owen, D Durnam, D Peters, D Pinkel, D Pinkel, Eduardo Diaz, Ernst W. Steegmayer, G Dudin, Gertrud Dudin, Haide Schnitzer, JC Fuscoe, JG Collard, K Nustad, Kathryn E. Howell, KE Howell, L Manuelidis, L-C Yu, M Schardin, Peter Vogt, PW Rigby, RS Molday, RV Lebo, T Lea, T Lea, Thomas Cremer, U Blochmann   +30 more
core   +1 more source

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

Nanomaterials, 2019
The surface functionalization of magnetic nanoparticles improves their physicochemical properties and applicability in biomedicine. Natural polymers, including proteins, are prospective coatings capable of increasing the stability, biocompatibility, and ...
Pavel Khramtsov, Irina Barkina, Maria Kropaneva, Maria Bochkova, Valeria Timganova, Anton Nechaev, Il’ya Byzov, Svetlana Zamorina, Anatoly Yermakov, Mikhail Rayev   +9 more
doaj   +1 more source

Electrophoretic behavior of streptavidin complexed to a biotinylated probe : A functional screening assay for biotin-binding proteins [PDF]

, 2005
The biotin-binding protein streptavidin exhibits a high stability against thermal denaturation, especially when complexed to biotin. Herein we show that, in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), streptavidin is stabilized ...
Humbert, Nicolas, Ward, Thomas R., Zocchi, Andrea   +2 more
core   +1 more source

Hybridization Chain Reaction-Based Electrochemical Biosensors by Integrating the Advantages of Homogeneous Reaction and Heterogeneous Detection

Biosensors, 2023
The conventional hybridization chain reaction (HCR)-based electrochemical biosensors usually require the immobilization of probes on the electrode surface.
Ning Xia, Jiayou Cheng, Linxu Tian, Shuo Zhang, Yunqiu Wang, Gang Li   +5 more
doaj   +1 more source

Fiber Optic Particle Plasmon Resonance-Based Immunoassay Using a Novel Multi-Microchannel Biochip

Sensors, 2020
A novel multi-microchannel biochip fiber-optic particle plasmon resonance (FOPPR) sensor system for the simultaneous detection of multiple samples.
Chang-Yue Chiang, Chien-Hsing Chen, Chien-Tsung Wang   +2 more
doaj   +1 more source

Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

Beilstein Journal of Nanotechnology, 2017
Gold nanoparticles (AuNPs) exhibit unique properties that can be modulated through a tailored surface functionalization, enabling their targeted use in biochemical sensing and medical diagnostics.
Roberta D'Agata, Pasquale Palladino, Giuseppe Spoto   +2 more
doaj   +1 more source