Results 31 to 40 of about 323,420 (331)

Real-Time Monitoring of Biotinylated Molecules Detection Dynamics in Nanoporous Anodic Alumina for Bio-Sensing

open access: yesNanomaterials, 2019
The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have
Laura Pol   +5 more
doaj   +1 more source

Strategies for the Biofunctionalization of Straining Flow Spinning Regenerated Bombyx mori Fibers

open access: yesMolecules, 2022
High-performance regenerated silkworm (Bombyx mori) silk fibers can be produced efficiently through the straining flow spinning (SFS) technique. In addition to an enhanced biocompatibility that results from the removal of contaminants during the ...
Paloma Lozano-Picazo   +18 more
doaj   +1 more source

Flap Endonuclease Disengages Dna2 Helicase/Nuclease from Okazaki Fragment Flaps [PDF]

open access: yes, 2006
Okazaki fragments contain an initiator RNA/DNA primer that must be removed before the fragments are joined. In eukaryotes, the primer region is raised into a flap by the strand displacement activity of DNA polymerase {delta}.
Bambara, Robert A.   +2 more
core   +1 more source

High-Yield Characterization of Single Molecule Interactions with DeepTipTM Atomic Force Microscopy Probes

open access: yesMolecules, 2022
Single molecule interactions between biotin and streptavidin were characterized with functionalized DeepTipTM probes and used as a model system to develop a comprehensive methodology for the high-yield identification and analysis of single molecular ...
Daniel Corregidor   +6 more
doaj   +1 more source

Heterogeneous and rate-dependent streptavidin-biotin unbinding revealed by high-speed force spectroscopy and atomistic simulations [PDF]

open access: yes, 2019
Receptor-ligand interactions are essential for biological function and their binding strength is commonly explained in terms of static lock-and-key models based on molecular complementarity.
Gonzalez, Laura   +4 more
core   +4 more sources

A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection

open access: yesBiosensors, 2021
The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal ...
Ping Ouyang   +8 more
doaj   +1 more source

Effects of High-Biotin Sample Interference on Antibody Concentrations in Sandwich Immunoassays

open access: yesVaccines, 2023
The use of antimicrobial growth promoters (AGPs) is banned because of problems associated with drug residues in animal products and increased bacterial resistance. The immunization of chickens with specific antigens is a promising strategy for generating
Geraldo Balieiro Neto   +4 more
doaj   +1 more source

Layer guided-acoustic plate mode biosensors for monitoring MHC-peptide interactions [PDF]

open access: yes, 2006
The transduction signals from the immobilisation of a class I heavy chain, HLA-A2, on a layer guided acoustic plate mode device, followed by binding of beta(2)-microglobulin and subsequent selective binding of a target peptide are ...
Dodi, I. A.   +7 more
core   +1 more source

Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection

open access: yesSensors, 2019
Aptamer biosensors are one of the most powerful techniques in biosensing. Achieving the best platform to use in aptamer biosensors typically includes crucial chemical modifications that enable aptamer immobilization on the surface in the most efficient ...
Laura Pol   +3 more
doaj   +1 more source

Insights into the structure of mature streptavidin C1 from Streptomyces cinnamonensis reveal the self-binding of the extension C-terminal peptide to biotin-binding sites

open access: yesIUCrJ, 2021
The members of the avidin protein family are well known for their high affinity towards d-biotin and their structural stability. These properties make avidins a valuable tool for various biotechnological applications.
Byeong Jun Jeon   +5 more
doaj   +1 more source

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