Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting‐out extraction system [PDF]
Engineering in Life Sciences, 2021 Clavulanic acid (CA) is usually used together with other β‐lactam antibiotics as combination drugs to inhibit bacterial β‐lactamases, which is mainly produced from the fermentation of microorganism such as Streptomyces clavuligerus. Recently, it is still
Xu‐Dong Wang +6 more
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Stringent response and initiation of secondary metabolism in Streptomyces clavuligerus [PDF]
Journal of General Microbiology, 1991 Cephalosporin biosynthetic activity and extracellular protease production increased during growth of Streptomyces clavuligerus in defined medium, while the level of guanosine 5'-diphosphate 3'-diphosphate (ppGpp) remained very low and stable. Cephalosporin biosynthesis (measured in resting cell systems) was initiated during early exponential growth in ...
V, Bascarán +3 more
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Dynamic metabolic modeling of Streptomyces clavuligerus in complex medium highlights nutrient-dependent metabolic transitions associated with clavulanic acid biosynthesis. [PDF]
PLoS ONEBackgroundStreptomyces clavuligerus is the main industrial producer of clavulanic acid (CA), a potent β-lactamase inhibitor. However, the metabolic interplay linking amino acid utilization, nitrogen regulation, and CA biosynthesis remains poorly ...
Leon F Toro-Navarro +2 more
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The CagRS Two-Component System Regulates Clavulanic Acid Metabolism via Multiple Pathways in Streptomyces clavuligerus F613-1 [PDF]
Frontiers in Microbiology, 2019 Streptomyces clavuligerus F613-1 produces a clinically important β-lactamase inhibitor, clavulanic acid (CA). Although the biosynthesis pathway of CA has essentially been elucidated, the global regulatory mechanisms of CA biosynthesis remain unclear. The
Jiafang Fu +6 more
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Effect of Soy Protein Products on Growth and Metabolism of Bacillus subtilis, Streptococcus lactis, and Streptomyces clavuligerus [PDF]
FoodsMicrobial nitrogen sources are promising, and soy protein as a plant-based nitrogen source has absolute advantages in creating microbial culture medium in terms of renewability, eco-friendliness, and greater safety.
Wei Wen +8 more
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Characterization of the Metabolic Response of Streptomyces clavuligerus to Shear Stress in Stirred Tanks and Single-Use 2D Rocking Motion Bioreactors for Clavulanic Acid Production [PDF]
Antibiotics, 2019 Streptomyces clavuligerus is a gram-positive filamentous bacterium notable for producing clavulanic acid (CA), an inhibitor of β-lactamase enzymes, which confers resistance to bacteria against several antibiotics.
David Gómez-Ríos +5 more
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Integrated genomic-transcriptomic analysis of clavulanic acid production in differentially productive Streptomyces clavuligerus strains [PDF]
Scientific ReportsClavulanic acid (CA) is a specific metabolite that inhibits β-lactamases, which inactivate β-lactam antibiotics, therefore reinstating the activity of β-lactams against β-lactamase-producing pathogens, and surmounting β-lactam resistance. While extensive
Junpyo Gong +10 more
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Efficient production of secretory Streptomyces clavuligerus β-lactamase inhibitory protein (BLIP) in Pichia pastoris [PDF]
AMB Express, 2018 β-Lactamase inhibitory protein (BLIP), a low molecular weight protein from Streptomyces clavuligerus, has a wide range of potential applications in the fields of biotechnology and pharmaceutical industry because of its tight interaction with and potent ...
Kin-Ho Law +7 more
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Functional insights into Streptomyces isolates containing both clavulanic acid-like and carbapenem biosynthetic gene clusters [PDF]
mSphereβ-Lactam antibiotics and β-lactamase inhibitor combinations are essential for combating antimicrobial resistance, with many β-lactams, including clavulanic acid (CA), themselves being products of specialized metabolic pathways in bacteria. CA is a potent
Kapil Tahlan +8 more
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Proline uptake inStreptomyces clavuligerus [PDF]
FEMS Microbiology Letters, 1990 Streptomyces clavuligerus was able to accumulate proline intracellularly throughout a wide range of external proline concentrations. Kinetic analysis of proline uptake indicated that this phenomenon is mediated by two saturable systems. One of them was a high-affinity system (Km = 11 microM), with low uptake capacity and specific for proline.
V, Bascarán, C, Hardisson, A F, Braña
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