Eliciting Clavulanic Acid Biosynthesis: The Impact of Bacillus velezensis FZB42 on the Metabolism of Streptoyces clavuligerus ATCC 27064 [PDF]
MetabolitesBackground/Objectives: Clavulanic acid (CA) is produced by cell suspension cultures of Streptomyces clavuligerus ATCC 27064, and is widely used as a beta-lactamase inhibitor to combat antibiotic resistance.
Luisa F. Patiño +4 more
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Functional insights into Streptomyces isolates containing both clavulanic acid-like and carbapenem biosynthetic gene clusters [PDF]
mSphereβ-Lactam antibiotics and β-lactamase inhibitor combinations are essential for combating antimicrobial resistance, with many β-lactams, including clavulanic acid (CA), themselves being products of specialized metabolic pathways in bacteria. CA is a potent
Kapil Tahlan +8 more
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Proline uptake inStreptomyces clavuligerus [PDF]
FEMS Microbiology Letters, 1990 Streptomyces clavuligerus was able to accumulate proline intracellularly throughout a wide range of external proline concentrations. Kinetic analysis of proline uptake indicated that this phenomenon is mediated by two saturable systems. One of them was a high-affinity system (Km = 11 microM), with low uptake capacity and specific for proline.
V, Bascarán, C, Hardisson, A F, Braña
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Protoplast Isolation and Regeneration in Streptomyces clavuligerus [PDF]
Microbiology, 1989 The regeneration of streptomycete protoplasts is a major step following genetic manipulations such as fusion and DNA-mediated transformation. Reports of studies on the regeneration of protoplasts from Streptomyces clavuligerus are limited and for this reason the experiments described in this paper were carried out.
G T, Illing +2 more
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A Study on the Production of Clavulanic Acid by Streptomyces clavuligerus using Carbon Resources such as Glucose, Glycerol, and Wheat Bran [PDF]
Biological Journal of Microorganism, 2022 Introduction: Clavulanic acid is the first B-Lactamase inhibitor that has been used clinically since 1981. This inhibitor is the natural product of the fermentation of Streptomyces clavuligerus and is useful for protecting B-lactams against lots of ...
Parisa Akhavan modares +2 more
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Genetic Mapping in Streptomyces clavuligerus by Protoplast Fusion [PDF]
Microbiology, 1989 The development of a protoplast manipulation protocol for the industrially important bacterium Streptomyces clavuligerus, which produces the beta-lactamase inhibitor clavulanic acid, made possible a preliminary genetic mapping study based on protoplast fusion crosses. A preliminary position for 11 markers on the S. clavuligerus genetic map is proposed.
G T, Illing +2 more
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Inhibition of Restriction in Streptomyces clavuligerus by Heat Treatment [PDF]
Microbiology, 1986 Inefficient transformation of Streptomyces clavuligerus protoplasts by DNA from the plasmid pIJ702, isolated from S. lividans, was attributed to restriction in view of the observation that efficient transformation was observed using modified pIJ702 (isolated from S. clavuligerus).
C R, Bailey, D J, Winstanley
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Genome-scale determination of 5´ and 3´ boundaries of RNA transcripts in Streptomyces genomes
Scientific Data, 2020 Measurement(s) 5´-ends of transcripts • 3´-ends of transcripts • RNA • TSS • transcription_termination_signal Technology Type(s) dRNA-Seq • Term-Seq • RNA sequencing Factor Type(s) Streptomyces growth phase Sample Characteristic - Organism Streptomyces ...
Yongjae Lee +7 more
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Frontiers in Microbiology, 2019 Clavulanic acid is a bacterial specialized metabolite, which inhibits certain serine β-lactamases, enzymes that inactivate β-lactam antibiotics to confer resistance.
Nader F. AbuSara +9 more
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Glycerol Utilization Gene Cluster in Streptomyces clavuligerus [PDF]
Applied and Environmental Microbiology, 2009 ABSTRACT The Streptomyces clavuligerus ATCC 27064 glycerol cluster gylR-glpF1K1D1 is induced by glycerol but is not affected by glucose. S. clavuligerus growth and clavulanic acid production are stimulated by glycerol, but this does not occur in a
Sonia, Baños +3 more
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