Results 31 to 40 of about 38,235 (184)

Protoplast Isolation and Regeneration in Streptomyces clavuligerus [PDF]

Microbiology, 1989
The regeneration of streptomycete protoplasts is a major step following genetic manipulations such as fusion and DNA-mediated transformation. Reports of studies on the regeneration of protoplasts from Streptomyces clavuligerus are limited and for this reason the experiments described in this paper were carried out.
G T, Illing, I D, Normansell, J F, Peberdy   +2 more
openaire   +2 more sources

Genetic Mapping in Streptomyces clavuligerus by Protoplast Fusion [PDF]

Microbiology, 1989
The development of a protoplast manipulation protocol for the industrially important bacterium Streptomyces clavuligerus, which produces the beta-lactamase inhibitor clavulanic acid, made possible a preliminary genetic mapping study based on protoplast fusion crosses. A preliminary position for 11 markers on the S. clavuligerus genetic map is proposed.
G T, Illing, I D, Normansell, J F, Peberdy   +2 more
openaire   +2 more sources

A Study on the Production of Clavulanic Acid by Streptomyces clavuligerus using Carbon Resources such as Glucose, Glycerol, and Wheat Bran [PDF]

Biological Journal of Microorganism, 2022
Introduction: Clavulanic acid is the first B-Lactamase inhibitor that has been used clinically since 1981. This inhibitor is the natural product of the fermentation of Streptomyces clavuligerus and is useful for protecting B-lactams against lots of ...
Parisa Akhavan modares, Fatemeh Ashrafi, Taher Nejadsattari   +2 more
doaj   +1 more source

Inhibition of Restriction in Streptomyces clavuligerus by Heat Treatment [PDF]

Microbiology, 1986
Inefficient transformation of Streptomyces clavuligerus protoplasts by DNA from the plasmid pIJ702, isolated from S. lividans, was attributed to restriction in view of the observation that efficient transformation was observed using modified pIJ702 (isolated from S. clavuligerus).
C R, Bailey, D J, Winstanley
openaire   +2 more sources

Genome-scale determination of 5´ and 3´ boundaries of RNA transcripts in Streptomyces genomes

Scientific Data, 2020
Measurement(s) 5´-ends of transcripts • 3´-ends of transcripts • RNA • TSS • transcription_termination_signal Technology Type(s) dRNA-Seq • Term-Seq • RNA sequencing Factor Type(s) Streptomyces growth phase Sample Characteristic - Organism Streptomyces ...
Yongjae Lee, Namil Lee, Soonkyu Hwang, Woori Kim, Yujin Jeong, Suhyung Cho, Bernhard O. Palsson, Byung-Kwan Cho   +7 more
doaj   +1 more source

Comparative Genomics and Metabolomics Analyses of Clavulanic Acid-Producing Streptomyces Species Provides Insight Into Specialized Metabolism

Frontiers in Microbiology, 2019
Clavulanic acid is a bacterial specialized metabolite, which inhibits certain serine β-lactamases, enzymes that inactivate β-lactam antibiotics to confer resistance.
Nader F. AbuSara, Brandon M. Piercey, Marcus A. Moore, Arshad Ali Shaikh, Louis-Félix Nothias, Santosh K. Srivastava, Pablo Cruz-Morales, Pieter C. Dorrestein, Francisco Barona-Gómez, Kapil Tahlan   +9 more
doaj   +1 more source

Improvement of clavulanic acid production in Streptomyces clavuligerus F613-1 by using a claR-neo reporter strategy

Electronic Journal of Biotechnology, 2017
Background: Streptomyces clavuligerus was the producer of clavulanic acid, claR, a pathway-specific transcriptional regulator in S. clavuligerus, positively regulates clavulanic acid biosynthesis.
Ronghuo Qin, Chuanqing Zhong, Gongli Zong, Jiafang Fu, Xiuhua Pang, Guangxiang Cao   +5 more
doaj   +1 more source

Optimized submerged batch fermentation strategy for systems scale studies of metabolic switching in Streptomyces coelicolor A3(2) [PDF]

, 2012
Background: Systems biology approaches to study metabolic switching in Streptomyces coelicolor A3(2) depend on cultivation conditions ensuring high reproducibility and distinct phases of culture growth and secondary metabolite production.
Bruheim, Per, Håvard Sletta, Hodgson, D. A., Alexander Wentzel, David A Hodgson, Anders Øverby, Sletta, Håvard, Øverby, Anders, Jakobsen, Øyvind Mejdell, Wentzel, Alexander, Walid A M Omara, Jakobsen, Øyvind M, Per Bruheim, Ellingsen, Trond E., Omara, Walid A. M., Hodgson, David A., Øyvind M Jakobsen, Ellingsen, Trond Erling, Trond E Ellingsen, Omara, Walid A.M.   +19 more
core   +1 more source

Proteomic Analysis of a hom-disrupted, Cephamycin C Overproducing Streptomyces clavuligerus.

, 2021
Background: Streptomyces clavuligerus is prolific producer of cephamycin C, a medically important antibiotic. In our former study, cephamycin C titer was 2-fold improved by disrupting homoserine dehydrogenase (hom) gene of aspartate pahway in ...
Becher, D, KURT KIZILDOĞAN, ASLIHAN, Becher, Dorte, Voigt, Birgit, Özcan, SERVET, Özcengiz, G, Kurt-Kızıldoğan, A, Özcengiz, Gülay, Aktas, Caner, Aktaş, C, Voigt, B, Ünsaldı, E, Unsaldi, Eser   +12 more
core   +1 more source

A Case of Convergent Evolution: The Bacterial Sesquiterpene Synthase for 1‐epi‐Cubenol from Nonomuraea coxensis

ChemBioChem, Volume 24, Issue 23, December 1, 2023., 2023
A high yielding sesquiterpene synthase for (+)‐1‐epi‐cubenol from the actinomycete Nonomuraea coxensis was investigated for its cyclisation mechanism by isotopic labelling experiments. Several derivatives of (+)‐1‐epi‐cubenol and of the plant derived compounds (−)‐cubenol and (−)‐1‐epi‐cubenol have been prepared, including an epoxide that is known as a
Jeroen S. Dickschat, Zhiyang Quan, Gregor Schnakenburg   +2 more
wiley   +1 more source