Results 51 to 60 of about 1,344 (153)
Overproduction of Clavulanic Acid by UV Mutagenesis of Streptomyces clavuligerus.
Iranian journal of pharmaceutical research : IJPR, 2010 Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces ...
Korbekandi, H +5 more
openaire +2 more sources
Regulatory mechanisms controlling antibiotic production in Streptomyces clavuligerus
Journal of Industrial Microbiology & Biotechnology, 2008 Streptomyces clavuligerus produces a large array of natural compounds with antibiotic, antitumor, beta-lactamase inhibition or inmunomodulating activities. The production of cephamycin C, clavulanic acid and other compounds with a clavam structure has been studied for many years. A network of regulatory mechanisms is present in S.
Paloma, Liras +2 more
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FEBS Letters, Volume 600, Issue 11, Page 1621-1637, June 2026.This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel +13 more
wiley +1 more source
Angewandte Chemie, Volume 136, Issue 21, May 21, 2024.Terpene synthases sense their substrates in a binary manner via one of two reactive oxygen atoms. We hypothesize that this alteration in binding, and subsequent chemistry, is due to these enzymes originating from plants or microorganisms. Abstract Terpene synthases (TPSs) catalyze the first step in the formation of terpenoids, which comprise the ...
Renana Schwartz, Shani Zev, Dan T. Major
wiley +2 more sources
BMC Genomics, 2017 Background Streptomyces clavuligerus ATCC 27064, the industrial producer of the β-lactamase inhibitor clavulanic acid, carries 49 putative secondary metabolite gene clusters.
Rubén Álvarez-Álvarez +3 more
doaj +1 more source
Transcriptional and translational analysis of the ccaR gene from Streptomyces clavuligerus [PDF]
Microbiology, 2004 CcaR is a positive-acting transcriptional regulator involved in cephamycin C and clavulanic acid biosynthesis in Streptomyces clavuligerus. Previous sequence analyses of the ccaR gene revealed two possible start codons, an ATG, and a GTG located in-frame 18 bp downstream of the ATG. To determine the true start codon, ccaR was expressed, either from the
Liru, Wang +4 more
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Enzymatic Synthesis of Demethylated Sesquiterpenes From 14‐nor‐Farnesyl Pyrophosphate
European Journal of Organic Chemistry, Volume 29, Issue 14, 15 April 2026.The substrate analog 14‐nor‐FPP was synthesised and enzymatically converted with 10 bacterial and fungal sesquiterpene synthases, resulting in the isolation of 12 demethylated sesquiterpenes in yields up to 17%. In most cases the demethyl analogs of the FPP‐derived natural products were obtained, but also different products showing modified ...
Kizerbo A. Taizoumbe +1 more
wiley +1 more source
Brazilian Archives of Biology and Technology, 2012 The aim of this work was to compare the composition of a complex and soluble culture medium to eight other media described in the literature through the batch cultivation in a conventional bench-scale bioreactor for the production of cephamycin C by a ...
Tatiana Antonio +5 more
doaj +1 more source
Phosphate regulation of ACV synthetase and cephalosporin biosynthesis in Streptomyces clavuligerus [PDF]
FEMS Microbiology Letters, 1989 Cephalosporin production by Streptomyces clavuligerus was reduced sharply by 60 mM phosphate added to a chemically-defined medium. All the four synthetases in the pathway examined, i.e., ACV synthetase, cyclase, epimerase and expandase, were repressed by phosphate, with ACV synthetase being the main repression target and expandase the next.
J, Jhang, S, Wolfe, A L, Demain
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The FEBS Journal, Volume 293, Issue 5, Page 1341-1357, March 2026.We have converted the only known true bacterial monoterpene synthase, cineole synthase from Streptomyces clavuligerus (bCinS, C10 substrate), to a highly competent sesquiterpene synthase (C15) with a minimum number of rational mutations. By comparison with diterpene synthases (C20), we were then able to bestow diterpene synthase activity on bCinS. This
Nicole G. H. Leferink +3 more
wiley +1 more source