Results 51 to 60 of about 38,235 (184)

Isolation and Characterization of Nitrogen-deregulated Mutants of Streptomyces clavuligerus [PDF]

Microbiology, 1989
Two screening methods for isolation of mutants of Streptomyces clavuligerus with altered control of nitrogen metabolism enzymes are described. Thirty-eight prototrophic mutants with simultaneous deregulation of urease and glutamine synthetase were isolated. Nine mutants were examined in more detail and they also showed deregulated formation of arginase
V, Bascarán, C, Hardisson, A F, Braña
openaire   +2 more sources

A quantitative study of the relationships between morphology, physiology and geldanamycin synthesis in submerged cultures of Streptomyces hygroscopicus var. geldanus [PDF]

, 2008
Microbially produced secondary metabolites such as antibiotics have tremendous economic importance. However, most are produced by filamentous organisms which exhibit diverse growth patterns presenting challenges for industrial fermentation.
Dobson, Lynne
core   +2 more sources

Biochemical and genetic investigations for the production of β-lactam metabolites from Streptomyces clavuligerus ATCC27064 and Streptomyces pratensis ATCC33331

, 2021
Streptomyces are recognized for their ability to produce a wide range of antimicrobial agents. In this thesis, two of the Streptomyces bacteria, Streptomyces clavuligerus and Streptomyces pratensis were the subject of my investigations.
AbuSara, Nader Fareid
core   +1 more source

Manipulation of the physiology of clavulanic acid production in Streptomyces clavuligerus [PDF]

Microbiology, 1997
This paper reports a novel use of cluster analysis for the identification of intermediary metabolites that are produced at rates closely correlated with those of antibiotic biosynthesis. This information was used to devise culture feeds resulting in enhanced production of clavulanic acid, an antibiotic of current worldwide commercial interest.
Paul R, Ives, Michael E, Bushell
openaire   +2 more sources

Regulatory mechanisms controlling antibiotic production in Streptomyces clavuligerus

Journal of Industrial Microbiology & Biotechnology, 2008
Streptomyces clavuligerus produces a large array of natural compounds with antibiotic, antitumor, beta-lactamase inhibition or inmunomodulating activities. The production of cephamycin C, clavulanic acid and other compounds with a clavam structure has been studied for many years. A network of regulatory mechanisms is present in S.
Paloma, Liras, Juan P, Gomez-Escribano, Irene, Santamarta   +2 more
openaire   +2 more sources

Overproduction of Clavulanic Acid by UV Mutagenesis of Streptomyces clavuligerus.

Iranian journal of pharmaceutical research : IJPR, 2010
Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces ...
Korbekandi, H, Darkhal, P, Hojati, Z, Abedi, D, Hamedi, J, Meraj Pourhosein, M   +5 more
openaire   +2 more sources

Scale‐up of Streptomyces species cultivations based on the morphological response to the energy dissipation rate

AIChE Journal, Volume 72, Issue 7, July 2026.
Abstract Filamentous microorganisms exhibit complex morphologies that influence product formation and are affected by various bioprocess parameters. Consistent morphology is therefore essential for comparable results during scale‐up. This study investigates the scale‐up of Streptomyces species (Streptomyces spp.) cultivations from shake flasks to ...
Gesa Brauneck, Anton Brass, Artiom Boreiko, Marvin Ueßeler, Marcel Mann, Johannes Pastoors, Valentin Steier, Philipp Eibl, Christian Witz, Dorothea M. Schütterle, Ivan Schlembach, Miriam A. Rosenbaum, Jorgen Magnus   +12 more
wiley   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, Volume 600, Issue 11, Page 1621-1637, June 2026.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Differential Substrate Sensing in Terpene Synthases from Plants and Microorganisms: Insight from Structural, Bioinformatic, and EnzyDock Analyses

Angewandte Chemie, Volume 136, Issue 21, May 21, 2024.
Terpene synthases sense their substrates in a binary manner via one of two reactive oxygen atoms. We hypothesize that this alteration in binding, and subsequent chemistry, is due to these enzymes originating from plants or microorganisms. Abstract Terpene synthases (TPSs) catalyze the first step in the formation of terpenoids, which comprise the ...
Renana Schwartz, Shani Zev, Dan T. Major
wiley   +2 more sources

Discovering the potential of S. clavuligerus for bioactive compound production: cross-talk between the chromosome and the pSCL4 megaplasmid

BMC Genomics, 2017
Background Streptomyces clavuligerus ATCC 27064, the industrial producer of the β-lactamase inhibitor clavulanic acid, carries 49 putative secondary metabolite gene clusters.
Rubén Álvarez-Álvarez, Yolanda Martínez-Burgo, Antonio Rodríguez-García, Paloma Liras   +3 more
doaj   +1 more source