Results 61 to 70 of about 38,235 (184)

Transcriptional and translational analysis of the ccaR gene from Streptomyces clavuligerus [PDF]

Microbiology, 2004
CcaR is a positive-acting transcriptional regulator involved in cephamycin C and clavulanic acid biosynthesis in Streptomyces clavuligerus. Previous sequence analyses of the ccaR gene revealed two possible start codons, an ATG, and a GTG located in-frame 18 bp downstream of the ATG. To determine the true start codon, ccaR was expressed, either from the
Liru, Wang, Kapil, Tahlan, Tracy L, Kaziuk, Dylan C, Alexander, Susan E, Jensen   +4 more
openaire   +2 more sources

Enzymatic Synthesis of Demethylated Sesquiterpenes From 14‐nor‐Farnesyl Pyrophosphate

European Journal of Organic Chemistry, Volume 29, Issue 14, 15 April 2026.
The substrate analog 14‐nor‐FPP was synthesised and enzymatically converted with 10 bacterial and fungal sesquiterpene synthases, resulting in the isolation of 12 demethylated sesquiterpenes in yields up to 17%. In most cases the demethyl analogs of the FPP‐derived natural products were obtained, but also different products showing modified ...
Kizerbo A. Taizoumbe, Jeroen S. Dickschat   +1 more
wiley   +1 more source

Evaluation of different media for the production of cephalosporins by Streptomyces clavuligerus ATCC 27064

Brazilian Archives of Biology and Technology, 2012
The aim of this work was to compare the composition of a complex and soluble culture medium to eight other media described in the literature through the batch cultivation in a conventional bench-scale bioreactor for the production of cephamycin C by a ...
Tatiana Antonio, Carolina Bellão, Tatiana Corrêa, André Pastrelo Cavallieri, Alberto Colli Badino, Maria Lucia Gonsales da Costa Araujo   +5 more
doaj   +1 more source

Phosphate regulation of ACV synthetase and cephalosporin biosynthesis in Streptomyces clavuligerus [PDF]

FEMS Microbiology Letters, 1989
Cephalosporin production by Streptomyces clavuligerus was reduced sharply by 60 mM phosphate added to a chemically-defined medium. All the four synthetases in the pathway examined, i.e., ACV synthetase, cyclase, epimerase and expandase, were repressed by phosphate, with ACV synthetase being the main repression target and expandase the next.
J, Jhang, S, Wolfe, A L, Demain
openaire   +2 more sources

Expanding the substrate scope of a bacterial monoterpene synthase for the production of sesquiterpenoid and diterpenoid products

The FEBS Journal, Volume 293, Issue 5, Page 1341-1357, March 2026.
We have converted the only known true bacterial monoterpene synthase, cineole synthase from Streptomyces clavuligerus (bCinS, C10 substrate), to a highly competent sesquiterpene synthase (C15) with a minimum number of rational mutations. By comparison with diterpene synthases (C20), we were then able to bestow diterpene synthase activity on bCinS. This
Nicole G. H. Leferink, Joshua N. Whitehead, Linus O. Johannissen, Nigel S. Scrutton   +3 more
wiley   +1 more source

Bibliometric Analysis of Global Research on Clavulanic Acid

Antibiotics, 2018
Clavulanic acid (CA), a potent inhibitor of the β-lactam, ase enzyme, is frequently co-formulated with a broad spectrum of antibiotics to treat infections caused by β-lactamase-producing pathogens.
Howard Ramirez-Malule
doaj   +1 more source

Efficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus [PDF]

Applied and Environmental Microbiology, 1987
The conditions for optimal formation and regeneration of protoplasts of Streptomyces clavuligerus were established. The optimal temperature for regeneration of protoplasts and for transformation was 26 degrees C in three different regeneration media. The best efficiency of transformation was obtained with 40% polyethylene glycol 1000.
M, Garcia-Dominguez, J F, Martin, B, Mahro, A L, Demain, P, Liras   +4 more
openaire   +2 more sources

A Review of β‐Lactamase Inhibitors in Clinical Use and Development: Mechanisms, Spectrum, and Therapeutic Applications

International Journal of Microbiology, Volume 2026, Issue 1, 2026.
β‐Lactam antibiotics have long served as a cornerstone for treating bacterial infections. However, their widespread and often indiscriminate use has fueled the emergence of multidrug‐resistant Gram‐negative pathogens, primarily through the production and dissemination of β‐lactamase enzymes.
Mohammad Javad Roustaye Gourabi, Anita Nikoo, Bita Khanbabaei, Masoud Kargar, Amirhossein Fayyazi, Seyyed Mohammad Javad Mousavi, Amirhossein Aghdaee, Ali Hashemi, Javad Yasbolaghi Sharahi, Swaran J. S. Flora   +9 more
wiley   +1 more source

Table_5_Activation of Secondary Metabolite Gene Clusters in Streptomyces clavuligerus by the PimM Regulator of Streptomyces natalensis.xlsx

, 2019
Expression of non-native transcriptional activators may be a powerful general method to activate secondary metabolites biosynthetic pathways. PAS-LuxR regulators, whose archetype is PimM, activate the biosynthesis of polyene macrolide antifungals and ...
Antonio Rodríguez-García (9375), Jesús F. Aparicio (160139), Eva G. Barreales (6057776), Andrew W. Truman (2835839), José Rubén Tormo (843826), Javier Santos-Aberturas (160115), Yolanda Martínez-Burgo (4615615), Fernando Reyes (444361), Paloma Liras (179701)   +8 more
core   +1 more source

The Roles of Carbon‐Nitrogen Synergy and Phosphate Regulation in Producing Higher Yield of Vancomycin by Amycolatopsis orientalis

MicrobiologyOpen, Volume 14, Issue 6, December 2025.
Systematic media optimization in Amycolatopsis orientalis identified maltodextrin, soy‐based nitrogen sources, and phosphate regulation as key drivers of vancomycin yield. Knockout and feeding trials demonstrated essential nutrient roles, offering a cost‐effective, scalable strategy for enhanced antibiotic production.
Vineeth Mani, Sivakamavalli Jeyachandran, Mohammed Aman   +2 more
wiley   +1 more source