AglH, a thermophilic UDP‑N‑acetylglucosamine‑1‑phosphate:dolichyl phosphate GlcNAc‑1‑phosphotransferase initiating protein N‑glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7 [PDF]
AglH, a predicted UDP-GlcNAc-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase, is initiating the protein N-glycosylation pathway in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. AglH successfully replaced the endogenous GlcNAc-1-
A Bouhss +73 more
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Cyclic nucleotide second messengers are increasingly implicated in prokaryotic anti-viral defence systems. Type III CRISPR systems synthesise cyclic oligoadenylate (cOA) upon detecting foreign RNA, activating ancillary nucleases that can be toxic to ...
Januka S Athukoralage +5 more
doaj +1 more source
Identification and characterization of small RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus. [PDF]
The term RNA silencing (RNA interference, RNAi) describes a set of mechanisms that regulate gene expression in eukaryotes. Small interfering RNAs (siRNA) and microRNAs (miRNAs) are two major types of RNAi-associated small RNAs (smRNAs) found in most ...
Ning Xu +8 more
doaj +1 more source
Improving the 'tool box' for robust industrial enzymes. [PDF]
This is the final version of the article. Available from Springer Verlag via the DOI in this record.The speed of sequencing of microbial genomes and metagenomes is providing an ever increasing resource for the identification of new robust biocatalysts ...
Littlechild, JA
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An affitin derived from the Sac7d scaffold enables the light‐controlled affinity purification of antibodies under physiological conditions, thus overcoming limitations of protein A chromatography. X‐ray crystallographic analysis and affinity measurements reveal complex formation with Fc at the CH2/CH3 junction, similar to protein A/G and FnRn. Combined
Felix Veitl +7 more
wiley +1 more source
Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.
Recent studies identified a 5´ to 3´ exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of β-CASP proteins. In
Birgit Märtens +7 more
doaj +1 more source
hSSB1 interacts directly with the MRN complex stimulating its recruitment to DNA double-strand breaks and its endo-nuclease activity [PDF]
hSSB1 is a recently discovered single-stranded DNA binding protein that is essential for efficient repair of DNA double-strand breaks (DSBs) by the homologous recombination pathway.
Bain, Amanda +7 more
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A Spotlight on Archaea in Humans, Livestock and Poultry: A Review
The microbiota includes prokaryotes (archaea and bacteria) and eukaryotes. Archaea are single‐celled prokaryotes and essential part of gut microbiome. Researches on archaea in ruminants and humans are more than mono‐gastric. The low abundance of archaea in the gut depends on the method used (metagenomics or meta‐transcriptomic) and age of people or ...
Ahmad Salahi, Wafaa A. Abd El‐Ghany
wiley +1 more source
Background: Sulphur-oxidizing microorganisms are widely used in the biofiltration of total reduced sulphur compounds (odorous and neurotoxic) produced by industries such as the cellulose and petrochemical industries, which include high-temperature ...
Emky Valdebenito-Rolack +4 more
doaj +1 more source
Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach [PDF]
People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
Ackermann, Katrin +6 more
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