Results 101 to 110 of about 15,229 (210)

Towards a Better Control of Engineered Circuit Transcription in Bacterial Genomes

Applied Microbiology
The transcription of genes and engineered circuits can deeply vary when inserted into different genomic loci. This unpredictable performance, termed context sensitivity, complicates strain development.
Nazim Sarica, Laurent Janniere, Brian C. Jester   +2 more
doaj   +1 more source

Autoregulation of topoisomerase I expression by supercoiling sensitive transcription

, 2016
The opposing catalytic activities of topoisomerase I (TopoI/relaxase) and DNA gyrase (supercoiling enzyme) ensure homeostatic maintenance of bacterial chromosome supercoiling.
Karthik, Pullela VDNB, Ahmed, Wareed, Menon, Shruti, Nagaraja, Valakunja   +3 more
core   +1 more source

Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

Nature Communications, 2017
The three-dimensional architecture of genome-reduced bacteria is poorly understood. Here the authors combine Hi-C with super-resolution microscopy inMycoplasma pneumoniaeand provide evidence of how supercoiling and local organization influences gene ...
Marie Trussart, Eva Yus, Sira Martinez, Davide Baù, Yuhei O. Tahara, Thomas Pengo, Michael Widjaja, Simon Kretschmer, Jim Swoger, Steven Djordjevic, Lynne Turnbull, Cynthia Whitchurch, Makoto Miyata, Marc A. Marti-Renom, Maria Lluch-Senar, Luís Serrano   +15 more
doaj   +1 more source

Supercoiling-mediated feedback rapidly couples and tunes transcription

Transcription induces a wave of DNA supercoiling, altering the binding affinity of RNA polymerases and reshaping the biochemical landscape of gene regulation. As supercoiling rapidly diffuses, transcription dynamically reshapes the regulation of proximal
Johnstone, Christopher P, Galloway, Kate E   +1 more
core   +1 more source

DNA supercoiling-mediated G4/R-loop formation tunes transcription by controlling the access of RNA polymerase

Nature Communications
RNA polymerase (RNAP) is a processive motor that modulates DNA supercoiling and reshapes DNA structures. The feedback loop between the DNA topology and transcription remains elusive.
Jihee Hwang, Chun-Ying Lee, Sumitabha Brahmachari, Shubham Tripathi, Tapas Paul, Huijin Lee, Alanna Craig, Taekjip Ha, Sua Myong   +8 more
doaj   +1 more source

Coordinated topoisomerase function shapes the fluoroquinolone response of Chlamydia trachomatis

Microbiology Spectrum
DNA supercoiling is essential for the developmental cycle of Chlamydia trachomatis, yet its role in shaping antibiotic responses remains poorly understood. We investigated how the fluoroquinolone moxifloxacin (Mox), a potent DNA gyrase inhibitor, affects
Andrew Terrebonne, Caitlynn Diggs, Scot P. Ouellette, Leiqiong Gao, Yuk-Ching Tse-Dinh, Li Shen   +5 more
doaj   +1 more source

Toward a Sensor for DNA Supercoiling

DNA in the cell takes on a double helical structure that is then formed into larger loops, compacting into a supercoil. These supercoils are the result of mechanical torsional forces caused by over or underwound DNA.
Kim, Payton Faith JungIn
core   +4 more sources

Bacterial genome architecture shapes global transcriptional regulation by DNA supercoiling

, 2019
International audienceDNA supercoiling acts as a global transcriptional regulator in bacteria, that plays an important role in adapting their expression programme to environmental changes, but for which no quantitative or even qualitative regulatory ...
Hindré, Thomas, Forquet, Raphaël, Reverchon, Sylvie, Meyer, Sam, El houdaigui, Bilal, Nasser, William, Schneider, Dominique   +6 more
core   +1 more source

Supercoiled roles [PDF]

Nature Reviews Genetics, 2013
openaire   +1 more source

Constrained thermal denaturation of DNA under fixed linking number

Open Physics, 2012
Bar Amir, Kabakçıoğlu Alkan, Mukamel David   +2 more
doaj   +1 more source