Results 101 to 110 of about 22,006 (218)
Microorganisms, 2019 Prokaryotes have an essential gene—gyrase—that catalyzes negative supercoiling of plasmid and chromosomal DNA. Negative supercoils influence DNA replication, transcription, homologous recombination, site-specific recombination, genetic ...
Nikolay S. Rovinskiy +3 more
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Supercoil formation in DNA denaturation
Physical Review E, 2009 We generalize the Poland-Scheraga (PS) model to the case of a circular DNA, taking into account the twisting of the two strains around each other. Guided by recent single-molecule experiments on DNA strands, we assume that the torsional stress induced by denaturation enforces formation of supercoils whose writhe absorbs the linking number expelled by ...
KABAKCIOGLU A +2 more
openaire +7 more sources
Variation of Structure and Cellular Functions of Type IA Topoisomerases across the Tree of Life
CellsTopoisomerases regulate the topological state of cellular genomes to prevent impediments to vital cellular processes, including replication and transcription from suboptimal supercoiling of double-stranded DNA, and to untangle topological barriers ...
Kemin Tan, Yuk-Ching Tse-Dinh
doaj +1 more source
Bioengineering, 2019 There is an increased interest in plasmid DNA as therapeutics. This is evident in the number of ongoing clinical trials involving the use of plasmid DNA.
Olusegun Folarin +3 more
doaj +1 more source
DNALiving systems are capable on the one hand of eliciting a coordinated response to changing environments (also known as adaptation), and on the other hand, they are capable of reproducing themselves.
Georgi Muskhelishvili +3 more
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Interplay of DNA supercoiling and catenation during the segregation of sister duplexes [PDF]
, 2017 The discrete regulation of supercoiling, catenation and knotting by DNA topoisomerases is well documented both in vivo and in vitro, but the interplay between them is still poorly understood. Here we studied DNA catenanes of bacterial plasmids arising as
Hernández, Pablo +5 more
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Towards a Better Control of Engineered Circuit Transcription in Bacterial Genomes
Applied MicrobiologyThe transcription of genes and engineered circuits can deeply vary when inserted into different genomic loci. This unpredictable performance, termed context sensitivity, complicates strain development.
Nazim Sarica +2 more
doaj +1 more source
Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae
Nature Communications, 2017 The three-dimensional architecture of genome-reduced bacteria is poorly understood. Here the authors combine Hi-C with super-resolution microscopy inMycoplasma pneumoniaeand provide evidence of how supercoiling and local organization influences gene ...
Marie Trussart +15 more
doaj +1 more source
Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling [PDF]
, 2017 DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is essential for DNA replication and gene regulation. However, the density of supercoiling in vivo is circa twice smaller than in deproteinized DNA molecules isolated ...
Benedetti, Fabrizio +7 more
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Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling [PDF]
, 2017 Reverse gyrase is an ATP-dependent topoisomerase that is unique to hyperthermophilic archaea and eubacteria. The only reverse gyrase structure determined to date has revealed the arrangement of the N-terminal helicase domain and the C-terminal ...
del Toro Duany, Yoandris +4 more
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