Results 11 to 20 of about 29,778 (263)
Neurobiology of Disease, 2020 Molecular changes at synapses are thought to underly the deficits in motor and cognitive dysfunction seen in Huntington's disease (HD). Previously we showed in synaptosome preparations age dependent changes in levels of selected proteins examined by ...
Ellen Sapp +6 more
doaj +2 more sources
Flow Cytometry Analysis and Quantitative Characterization of Tau in Synaptosomes from Alzheimer’s Disease Brains [PDF]
Methods in molecular biology, 2016 Synaptosomes, resealed nerve terminals that form when tissue is homogenized in isotonic medium, are a model system that has been a key source of knowledge about neurotransmission.
Gylys, Karen Hoppens +3 more
core +2 more sources
Cryoloading: Introducing Large Molecules into Live Synaptosomes
Frontiers in Cellular Neuroscience, 2014 Neurons communicate with their target cells primarily by the release of chemical transmitters from presynaptic nerve terminals. The study of CNS presynaptic nerve terminals, isolated as synaptosomes (SSMs) has, however, been hampered by the typical ...
Arup R Nath +2 more
doaj +2 more sources
Intrinsic Bioenergetic Properties and Stress Sensitivity of Dopaminergic Synaptosomes
Journal of Neuroscience, 2011 Dopaminergic neurons of the substantia nigra pars compacta are defective in Parkinson's disease, but the specificity of this dysfunction is not understood.
Akos A. Gerencser +6 more
core +2 more sources
Reorganization of actin in depolarized synaptosomes [PDF]
The Journal of Neuroscience, 1985 Depolarization of whole brain synaptosomes, which stimulates transmitter release, also affects regulation of the assembly of actin microfilaments. Lysates of depolarized synaptosomes contain 20% less cytoskeletal actin than lysates of unstimulated synaptosomes.
B W, Bernstein, J R, Bamburg
openaire +3 more sources
Preparation, Stimulation and Other Uses of Adult Rat Brain Synaptosomes
Bio-Protocol, 2017 In this paper, our protocol for preparation of brain synaptosomes is described. Synaptosomes are a valuable model system for analysis of structural components of the synapse as well as for investigation of synaptic function. Synaptosomal preparations are
Jigar Modi, Howard Prentice, Jang-Yen Wu
doaj +2 more sources
Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening
Nature Protocols, 2012 Small molecules modulating synaptic vesicle endocytosis (SVE) may ultimately be useful for diseases where pathological neurotransmission is implicated. Only a small number of specific SVE modulators have been identified to date.
McCluskey, Adam +4 more
core +2 more sources
Distinct Effects of Nonselective Rho-Kinase Inhibitor Fasudil and Selective Rho-Kinase 2 Inhibitor KD025 on Serotonin and Dopamine Release in the Nucleus Accumbens of Mice. [PDF]
Neuropsychopharmacol RepFasudil perfusion in the NAc increased basal extracellular serotonin but not dopamine, and suppressed depolarization‐evoked release of both. In contrast, KD025 showed minimal effects on basal levels and selectively suppressed evoked serotonin release. ABSTRACT Recent studies have indicated that the Rho GTPase family and Rho‐kinases are associated with ...
Tanaka R +8 more
europepmc +2 more sources
Synaptosome microRNAs regulate synapse functions in Alzheimer’s disease
npj Genomic Medicine, 2022 MicroRNAs (miRNAs) are found in nerve terminals, synaptic vesicles, and synaptosomes, but it is unclear whether synaptic and cytosolic miRNA populations differ in Alzheimer’s disease (AD) or if synaptosomal miRNAs affect AD synapse activity.
Subodh Kumar +6 more
doaj +1 more source
Frontiers in Pharmacology, 2020 We investigated whether chronic sciatic ligation modifies the glutamate release in spinal cord nerve endings (synaptosomes) as well as the expression and the function of presynaptic release-regulating mGlu2/3 autoreceptors and 5-HT2A heteroreceptors in ...
Francesca Cisani +7 more
doaj +1 more source