Results 71 to 80 of about 1,916,955 (263)

Choice of activation protocol impacts the yield and quality of CAR T cell product, particularly with older individuals

Clinical & Translational Immunology
Objectives In clinical chimeric antigen receptor (CAR) T cell therapy, one of the strongest correlates of favorable patient responses is lower levels of differentiation in T cells from the peripheral blood mononuclear cell (PBMC) starting material or the
Palak H Mehta, Gemma S Trollope, Patrick Leung, Shivali Savita Chinni, Anna Iasinskaia, Aaron J Harrison, Hannah Hughes‐Parry, Misty R Jenkins, Michael H Kershaw, Anthony Jaworowski, Clare Y Slaney, Rachel M Koldej, David S Ritchie, Kylie M Quinn   +13 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Analysis of significance of CARD11 and MYO1G expressions in pulmonary tuberculosis and their predictive value for prognosis of recurrence [PDF]

Journal of Medical Biochemistry
Pulmonary tuberculosis (PTB) is one of the most common infectious diseases worldwide, with extremely high morbidity and mortality. An in-depth understanding of the molecular pathogenesis of PTB is crucial for finding novel diagnostic and therapeutic ...
Liu Fengxia, Lin Shengxun, Li Li, Xu Bei, Chang Tingting   +4 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Cullin-5 deficiency promotes chimeric antigen receptor T cell effector functions potentially via the modulation of JAK/STAT signaling pathway

Nature Communications
Chimeric antigen receptor (CAR) T cell is a promising therapy for cancer, but factors that enhance the efficacy of CAR T cell remain elusive. Here we perform a genome-wide CRISPR screening to probe genes that regulate the proliferation and survival of ...
Yoshitaka Adachi, Seitaro Terakura, Masahide Osaki, Yusuke Okuno, Yoshitaka Sato, Ken Sagou, Yuki Takeuchi, Hirofumi Yokota, Kanae Imai, Peter Steinberger, Judith Leitner, Ryo Hanajiri, Makoto Murata, Hitoshi Kiyoi   +13 more
doaj   +1 more source

T Cell Resistance: On the Mechanisms of T Cell Non-activation

Immune Network
Immunological tolerance is a fundamental arm of any functioning immune system. Not only does tolerance mitigate collateral damage from host immune responses, but in doing so permits a robust response sufficient to clear infection as necessary. Yet, despite occupying such a cornerstone, research aiming to unravel the intricacies of tolerance induction ...
Daniel Beckers, Ashwin K. Jainarayanan, Michael L. Dustin, Jesusa Capera   +3 more
openaire   +2 more sources

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Recent insights of T cell receptor-mediated signaling pathways for T cell activation and development

Experimental and Molecular Medicine, 2020
Immunology: The signals that turn on T cells Researchers are unravelling the molecular signaling pathways that activate T cells, white blood cells which play a central role in the immune response. Sung-Gyoo Park and colleagues at the Gwangju Institute of
Jeong-Ryul Hwang, Yeongseon Byeon, Donghwan Kim, Sung-Gyoo Park   +3 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Regulation of T cell differentiation and function by EZH2

Frontiers in Immunology, 2016
The enhancer of zeste homologue 2 (EZH2), one of the polycomb group (PcG) proteins, is the catalytic subunit of Polycomb-repressive complex 2 (PRC2) and induces the trimethylation of the histone H3 lysine 27 (H3K27me3) promoting epigenetic gene silencing.
THEODOROS KARANTANOS, THEODOROS KARANTANOS, Anthos Christofides, Kankana Bardhan, Lequn Li, Lequn Li, Vassiliki A Boussiotis   +6 more
doaj   +1 more source