Results 61 to 70 of about 1,916,955 (263)

T Cell Activation through Isolated Tight Contacts

Cell Reports, 2019
Summary: T cells engage antigen-presenting cells in search for cognate antigens via dynamic cell protrusions before forming a tight immune synapse. The spatiotemporal events that may lead to rapid TCR triggering and signal amplification in microvilli ...
Yair Razvag, Yair Neve-Oz, Julia Sajman, Oren Yakovian, Meital Reches, Eilon Sherman   +5 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Loss of ATG5 impairs CD4+ T cell activation and promotes anti-tumor responses

Frontiers in Immunology
Macroautophagy (hereafter called autophagy) is an ancient catabolic process that delivers bulky cargo to lysosomal degradation. The autophagic pathway is regulated by autophagy-related (ATG) proteins that govern the formation of double-membraned vesicles
Carlos Plaza-Sirvent, Clara Bessen, Alisha W. Bronietzki, Alisha W. Bronietzki, Katjana Klages, Marc Schuster, Marc Schuster, Jochen Huehn, Ingo Schmitz   +8 more
doaj   +1 more source

T Cell Activation Depends on Extracellular Alanine

Cell Reports, 2019
Summary: T cell stimulation is metabolically demanding. To exit quiescence, T cells rely on environmental nutrients, including glucose and the amino acids glutamine, leucine, serine, and arginine.
Noga Ron-Harel, Jonathan M. Ghergurovich, Giulia Notarangelo, Martin W. LaFleur, Yoshiki Tsubosaka, Arlene H. Sharpe, Joshua D. Rabinowitz, Marcia C. Haigis   +7 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Cytoskeletal Control of Antigen-Dependent T Cell Activation

Cell Reports, 2019
Summary: Cytoskeletal actin dynamics is essential for T cell activation. Here, we show evidence that the binding kinetics of the antigen engaging the T cell receptor influences the nanoscale actin organization and mechanics of the immune synapse.
Huw Colin-York, Yousef Javanmardi, Mark Skamrahl, Sudha Kumari, Veronica T. Chang, Satya Khuon, Aaron Taylor, Teng-Leong Chew, Eric Betzig, Emad Moeendarbary, Vincenzo Cerundolo, Christian Eggeling, Marco Fritzsche   +12 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

T cell antigenic recognition sequencing (TAR-seq) enables precise characterization of vaccine-elicited cellular immunity ex vivo and in vivo

Frontiers in Immunology
IntroductionThe functional responses of antigen (Ag) specific T cells are complex, clonotype-specific, context-dependent, and incompletely captured by dominant analytic approaches.
Morgan Chaunzwa, GW McElfresh, David Morrow, Gregory J. Boggy, Maanasa Kaza, Sebastian Benjamin, Shana Feltham, Sohita Ojha, Gwendolyn M. Swarbrick, Katherine H. Rott, Roxanne M. Gilbride, Caralyn S. Labriola, Caralyn S. Labriola, Michael K. Axthelm, Michael K. Axthelm, Vineet Joag, Vineet Joag, Dylan Kain, Nelita Du Plessis, Gerhard Walzl, Deborah A. Lewinsohn, David M. Lewinsohn, Scott G. Hansen, Scott G. Hansen, Louis J. Picker, Louis J. Picker, Benjamin N. Bimber, Benjamin N. Bimber   +27 more
doaj   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source