Results 11 to 20 of about 10,148 (237)
TcMYB29a, an ABA-Responsive R2R3-MYB Transcriptional Factor, Upregulates Taxol Biosynthesis in Taxus chinensis [PDF]
Frontiers in Plant Science, 2022 Paclitaxel (Taxol), a highly modified diterpene agent mainly obtained from Taxus species, is the most widely used anticancer drug. Abscisic acid (ABA) is a well-known stress hormone that plays important roles in the secondary metabolism of plants, and it
Xiaoying Cao +4 more
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Cytochrome P450 oxygenases of Taxol biosynthesis [PDF]
Phytochemistry Reviews, 2006 Cytochrome P450 monooxygenases play a prominent role in the biosynthesis of the diterpenoid anticancer drug Taxol, as they appear to constitute about half of the 19 enzymatic steps of the pathway in yew (Taxus) species. A combination of classical biochemical and molecular methods, including cell-free enzyme studies and differential-display of mRNA ...
Rüdiger Kaspera, Rodney Croteau
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Insights into Taxol® biosynthesis by endophytic fungi [PDF]
Applied Microbiology and Biotechnology, 2023 Abstract There have been two hundred reports that endophytic fungi produce Taxol®, but its production yield is often rather low. Although considerable efforts have been made to increase Taxol/taxanes production in fungi by manipulating cocultures, mutagenesis, genome shuffles, and gene overexpression, little is known ...
Subban Kamalraj, Frank Kempken
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Chemistry & Biology, 2004 Biosynthesis of the anticancer drug Taxol in yew species involves eight cytochrome P450-mediated oxygenations and four coenzyme A-dependent acylations of the diterpenoid core. A family of cytochrome P450 genes, obtained from a yew cell cDNA library, were functionally expressed and screened with taxusin (taxa-4(20),11(12)-dien-5 alpha,9 alpha,10 beta,13
MyDoanh Chau +3 more
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Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species [PDF]
BMC Plant Biology, 2019 Background Taxol is an efficient anticancer drug; however, the accumulation of taxoids can vary hugely among Taxus species. The mechanism underlying differential accumulation of taxoids is largely unknown. Thus, comparative analysis of the transcriptomes
Ting Zhou +7 more
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Taxol biosynthesis: Taxane 13α-hydroxylase is a cytochrome P450-dependent monooxygenase [PDF]
Proceedings of the National Academy of Sciences, 2001 A central feature in the biosynthesis of Taxol is oxygenation at multiple positions of the taxane core structure, reactions that are considered to be mediated by cytochrome P450-dependent monooxygenases. A PCR-based differential display-cloning approach, usingTaxus(yew) cells induced for Taxol production, yielded a family of related cytochrome P450 ...
Stefan Jennewein +3 more
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TcMYC2a, a Basic Helix–Loop–Helix Transcription Factor, Transduces JA-Signals and Regulates Taxol Biosynthesis in Taxus chinensis [PDF]
Frontiers in Plant Science, 2018 The multitherapeutic taxol, which can be obtained from Taxus spp., is the most widely used anticancer drug. Taxol biosynthesis is significantly regulated by jasmonate acid (JA), one of the most important endogenous hormones in land plants.
Meng Zhang +15 more
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Cloning, Heterologous Expression, and Characterization of a Phenylalanine Aminomutase Involved in Taxol Biosynthesis [PDF]
Journal of Biological Chemistry, 2004 Biosynthesis of the N-benzoyl phenylisoserinoyl side chain of the anticancer drug Taxol starts with the conversion of 2S-alpha-phenylalanine to 3R-beta-phenylalanine by phenylalanine aminomutase (PAM). A gene cloning approach was based on the assumption that PAM would resemble the well known plant enzyme phenylalanine ammonia lyase.
Kevin D. Walker +3 more
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Cloning and Sequencing of Hydroxylase Genes Involved in Taxol Biosynthesis [PDF]
Zeitschrift für Naturforschung C, 2004 Abstract Two full-length cDNAs (TCH1 and TCH2) were obtained from a cDNA library of Taxus chinensis mainly by the single specific-primer PCR (SSP-PCR) method. Compared with other reported enzymes from Taxus species, the deduced amino acid sequences of TCH1 and TCH2 exhibit significant homologies to hydroxylases that are involved in taxol
Jun Tu, Ping Zhu, Ke-Di Cheng, Chao Meng
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Journal of Microbiology and Biotechnology, 2019 The fungal products dibenzodioxocinones promise a novel class of inhibitors against cholesterol ester transfer protein (CEPT). Knowledge as to their biosynthesis is scarce. In this report, we characterized four more dibenzodioxocinones, which along with a previously described member pestalotiollide B, delimit the dominant spectrum of secondary ...
Yanjie Liu +9 more
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