Results 21 to 30 of about 10,148 (237)

Studies on the biosynthesis of taxol: the taxane carbon skeleton is not of mevalonoid origin. [PDF]

Proceedings of the National Academy of Sciences, 1996
A cell culture of Taxus chinensis was established to produce the diterpene 2alpha,5alpha,10beta,14beta-tetra-acetoxy4 ++ +(20),11-taxadiene (taxuyunnanine C) in 2.6% (dry weight) yield. The incorporation of [U-13C6]glucose, [1-13C]glucose, and [1,2-13C2]acetate into this diterpene was analyzed by NMR spectroscopy.
Wolfgang Eisenreich, Birgitta Menhard, Peter J. Hylands, Meinhart H. Zenk, A. Bacher   +4 more
openalex   +4 more sources

Fungal endophytes of Taxus species and regulatory effect of two strains on taxol synthesis [PDF]

BMC Microbiology
Background Taxol, derived from Taxus trees, is a valuable natural resource for the development of anticancer drugs. Endophytic fungi from Taxus trees are a promising alternative source of Taxol.
Hongshan Zhang, Wanting Lin, Ruoyun Ma, Yue Zang, Kailin Hou, Zhen Xu, Xiaoyun Xi, Weiting Zhang, Shini Tang, Xueshuang Liang, Yiming Sun, Chenjia Shen   +11 more
doaj   +2 more sources

Screening of taxol biosynthesis-related genes in taxol produced from Nodulisporium sylviforme HDF-68 by mRNA differential display [PDF]

Annals of Microbiology, 2014
Differential expressed genes in taxol producing Nodulisporium sylviforme HDF-68 at different periods of fermentation were analyzed by mRNA differential display. A total of 38 fragments were found expressed differentially during taxol synthesis phase. Using reverse Northern blot analysis, the hybridization signals of 16 fragments were consistent with ...
Kai Zhao, Ye Xiao, Chao Wang, Dan Liu, Yue Zhang, Xin Wang, Xiuliang Li, Tao Jin   +7 more
openalex   +3 more sources

Plant synthetic biology-based biofortification, strategies and recent progresses. [PDF]

J Integr Plant Biol
This mini‐review highlights fi ve synthetic biology strategies to boost crop nutrition and alleviate the ‘hidden hunger’ resulting from vitamin and nutrient defi ciencies. These approaches offer precise, sustainable ways to engineer nutrient‐rich plants, providing innovative solutions to global malnutrition through biofortifi cation.
Wang K, Liu Z.
europepmc   +2 more sources

Amesia nigricolor, a novel endophyte of Encephalartos bubalinus, exhibiting a robust taxol biosynthetic stability: chemical characterization and biological activities [PDF]

Microbial Cell Factories
Diminishing the productivity of Taxol by the potential fungi with storage is the key hurdle that impedes their applications to be an industrial platform for Taxol production.
Asmaa Gamal, Ashraf S. A. El-Sayed, Eman Fikry, Nora Tawfeek, Azza M. El-Shafae, Maher M. El-Domiaty   +5 more
doaj   +2 more sources

Biosynthesis of the Red Algal Diterpene Peyssonnosol in Bacteria. [PDF]

Angew Chem Int Ed Engl
Two terpene synthases derived from Anaerolineae bacteria catalyse the biosynthesis of peyssonnosene‐type diterpenes from red algae. Site‐directed mutagenesis enabled a partial functional switch, resulting in the formation of additional byproducts. The intricate cyclisation mechanisms were investigated through isotopic labelling experiments and density ...
Yin Z, Hu Z, Yang K, Lu G, Tabekoueng GB, Wu P, Luo Q, Xu J, Goldfuss B, Dickschat JS, Bian G.   +10 more
europepmc   +2 more sources

Studies on Taxol biosynthesis: preparation of taxadiene-diol and triol derivatives by deoxygenation of taxusin [PDF]

Tetrahedron, 2008
The putative taxol biosynthesis metabolites, taxa-4(20),11(12)-diene-5α, 13α -diol (7), taxa-4(20),11(12)-diene-5α, 9α, 13α-triol (9), and taxa-4(20),11(12)-diene-5α, 10β, 13α-triol (10), have been prepared by Barton deoxygenation of the C-9 and C10-hydroxyl groups of protected derivatives of taxusin, a major taxoid metabolite isolated from Yew heart ...
Hui Li, Tohru Horiguchi, Rodney Croteau, Robert M. Williams   +3 more
openalex   +3 more sources

Transcriptome and Metabolome Analyses of Taxus chinensis var. mairei Tissues Provide New Insights into the Regulation of Paclitaxel Biosynthesis [PDF]

Plants
Taxus is the natural source of the anticancer drug paclitaxel. Although significant progress has been made in elucidating the biosynthetic pathway of paclitaxel, its tissue-specific accumulation and associated regulatory networks in Taxus remains unclear.
Luyuan Jiang, Yanyan Li, Xiaoyang Jiang, Fenjuan Shao, Wenli Wu, Fan Xu, Iain Wilson, Angela Hoffman, Yanfang Yang, Deyou Qiu   +9 more
doaj   +2 more sources

Taxol biosynthesis: Identification and characterization of two acetyl CoA:taxoid-O-acetyl transferases that divert pathway flux away from Taxol production [PDF]

Archives of Biochemistry and Biophysics, 2009
Two cDNAs encoding taxoid-O-acetyl transferases (TAX 9 and TAX 14) were obtained from a previously isolated family of Taxus acyl/aroyl transferase cDNA clones. The recombinant enzymes catalyze the acetylation of taxadien-5alpha,13alpha-diacetoxy-9alpha,10beta-diol to generate taxadien-5alpha,10beta,13alpha-tri-acetoxy-9alpha-ol and taxadien-5alpha ...
Daniela Hampel, Christopher J.D. Mau, Rodney Croteau   +2 more
openalex   +4 more sources

Engineering cyanobacteria as a new platform for producing taxol precursors directly from carbon dioxide [PDF]

Biotechnology for Biofuels and Bioproducts
Taxol serves as an efficient natural anticancer agent with extensive applications in the treatment of diverse malignancies. Although advances in synthetic biology have enabled the de novo synthesis of taxol precursors in various microbial chassis, the ...
Jialing Zhong, Yushu Wang, Zhuoyang Chen, Yaliqin Yalikun, Lin He, Tiangang Liu, Gang Ma   +6 more
doaj   +2 more sources