Results 111 to 120 of about 291,656 (309)

In-Cell NMR Characterization of the Secondary Structure Populations of a Disordered Conformation of α-Synuclein within E. coli Cells. [PDF]

, 2013
α-Synuclein is a small protein strongly implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders. We report here the use of in-cell NMR spectroscopy to observe directly the structure and dynamics of this protein ...
Christopher A. Waudby (238969), A. W. P. Fitzpatrick, Cabrita, LD, Carlo Camilloni, C. A. Waudby, Cabrita Lisa D., Camilloni Carlo, Christodoulou, J, Waudby Christopher A., Christodoulou, John, John Christodoulou, Camilloni, C., Christopher M Dobson, Lisa D. Cabrita, Waudby, CA, Dobson Christopher M., Michele Vendruscolo (68357), Temussi, P.A., Lisa D Cabrita, Fitzpatrick, AW, Christopher M. Dobson, Christopher M. Dobson (181572), Fitzpatrick, A.W.P., Dobson, C.M., Anthony W. P. Fitzpatrick, John Christodoulou (450398), Dobson, CM, C. Camilloni, Cabrita, L.D., Vendruscolo, M, Anthony W. P. Fitzpatrick (450396), M. Vendruscolo, Carlo Camilloni (450395), Anthony W P Fitzpatrick, Lisa D. Cabrita (450397), L. D. Cabrita, Christodoulou John, C. M. Dobson, Waudby, Christopher A., J. Christodoulou, Camilloni, C, Fitzpatrick Anthony W. P., Christopher A Waudby, Michele Vendruscolo, Christopher A. Waudby, Vendruscolo Michele, Vendruscolo, M.   +46 more
core   +1 more source

Interpreting the effects of DNA polymerase variants at the structural level

Molecular Oncology, EarlyView.
Using MAVISp and molecular dynamics simulations, we analyzed over 60 000 missense variants in POLE and POLD1 from ClinVar, COSMIC, cBioPortal, and saturation mutagenesis. Identified mechanistic indicators, including stability, binding, and long‐range, enable structural interpretation, providing ACMG‐like evidence for possible reclassification of VUS ...
Matteo Arnaudi, Karolina Krzesińska, Ludovica Beltrame, Pablo Sánchez‐Izquierdo Besora, Matteo Tiberti, Mef Nilbert, Anna Rohlin, Elena Papaleo   +7 more
wiley   +1 more source

Disulfide trapping analysis of AcrB tertiary structure.

, 2013
A. Schematic illustration of the blocking-reducing-labeling procedure. B. The locations of reporter Cys pairs in the structure of AcrB were highlighted using black circles and blue ball-and-stick models.
Linliang Yu (338349), Yinan Wei (338350), Wei Lu (3580)   +2 more
core   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

Tertiary lymphoid structures in gliomas: impact on tumour immunity and progression

Journal of Translational Medicine
Tertiary lymphoid structures (TLSs) are ectopic lymphoid formations that develop in chronically inflamed tissues, including various solid tumours. In the context of gliomas, the presence of TLSs has recently attracted considerable attention because of ...
Jiatong Chen, Yuechao Yang, Shuang Luan, Wenhao Xu, Yang Gao   +4 more
doaj   +1 more source

Tumor B‐cell infiltration in platinum‐treated advanced muscle‐invasive urothelial carcinoma

Molecular Oncology, EarlyView.
Bladder tumors with higher pretreatment memory B‐cell infiltration were linked to longer survival after cisplatin chemotherapy, but not carboplatin. These tumors also showed more organized immune structures (tertiary lymphoid structures) and a shared pro‐inflammatory B‐cell‐rich community, suggesting that memory B cells may help identify patients most ...
Konrad Stawiski, Júlia Perera‐Bel, Alejo Rodriguez‐Vida, Nuria Juanpere, Jihyun Lee, Daniel E. Michaud, Jennifer L. Guerriero, Kent W. Mouw, Aristotelis Bamias, Filipe L. F. Carvalho, Joaquim Bellmunt   +10 more
wiley   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Spatial Differentiation and Influencing Factors of Tertiary Industry in the Pearl River Delta Urban Agglomeration

Land
The tertiary industry has become the main driving force for China’s economic development, and the adjustment and optimization of its structure are important prerequisites for achieving high-quality economic development.
Xiaodie Yuan, Baoyu Chen, Xiong He, Guojun Zhang, Chunshan Zhou   +4 more
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source