Results 51 to 60 of about 13,537 (210)

Cloning and expression of the membrane protein gene of TGEV HB06 strain [PDF]

Frontiers of Agriculture in China, 2010
The membrane protein gene(M) of transmissible gastroenteritis virus (TGEV) strain HB06, isolated from the feces of piglets infected with TGEVon a pig farm in Hebei province, was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). The amplified PCR products of TGEV HB06 were cloned, sequenced, and compared with other TGEV strains ...
Zhang, Xiaobo, Zuo, Yuzhu, Fan, Jinghui, Liu, Yuan   +3 more
openaire   +2 more sources

Ultrasensitive Detection of RNA and DNA Viruses Simultaneously Using Duplex UNDP-PCR Assay. [PDF]

PLoS ONE, 2015
Mixed infection of multiple viruses is common in modern intensive pig rearing. However, there are no methods available to detect DNA and RNA viruses in the same reaction system in preclinical level.
Yong Huang, Na Xing, Zengguo Wang, Xiujuan Zhang, Xiaomin Zhao, Qian Du, Lingling Chang, Dewen Tong   +7 more
doaj   +1 more source

Antibody levels in mice treated with pIRES-(TGEV-S1-PEDV-S1) and pIRES-(TGEV-S1-PEDV-S).

, 2013
Anti-PEDV serum antibodies (A) and anti-TGEV serum antibodies (B) in pIRES-(TGEV-S1-PEDV-S1) and pIRES-(TGEV-S1-PEDV-S) immunized mice were detected by indirect ELISA at different time points following the injection of the plasmid DNAs.
Georg Herrler (199104), Xunliang Li (351925), Siqingaowa Suo (302566), Pengchong Li (391249), Wei Yang (109917), Guangxing Li (338141), Xuejiao Sun (391248), Christel Schwegmann-Wessels (5652643), Yudong Ren (302564), Fandan Meng (351921), Xiaofeng Ren (302569), Lu Li (14069)   +11 more
core   +1 more source

The canonical autophagy pathway does not participate in inhibiting TGEV replication.

, 2021
(Left) Western blot analysis of LC3-I and LC3-II in untreated WT and ATG5 KO cells; (Middle) Western blot analysis of LC3-I and LC3-II in WT and ATG5 KO cells after EBSS medium (EBSS) starvation and E64d supplementation for 6 h; (Right) Western blot ...
Limeng Sun (9321756), Jinfu Zhang (709197), Jingjin Li (4172617), Xiongwei Nie (9321744), Guiqing Peng (90055), Zhelin Su (11797970), Changzhi Zhao (1647001), Yanan Fu (4422967), Yuan Zhou (179597), Shengsong Xie (582082), Yubei Tan (11797973), Shuhong Zhao (48168), Yangyang Li (554659), Zhen Fu (669777), Yixin Xiang (11797976), Fei Liu (21082)   +15 more
core   +1 more source

TMEM41B KO ST cells show inhibited TGEV replication.

, 2021
(A) WT cells and VMP1 KO PK-15 cells were infected with TGEV (MOI = 1) for 24h and used for immunofluorescence assays. Left: representative pictures. Right: quantification of TGEV N positive cells.
Limeng Sun (9321756), Jinfu Zhang (709197), Jingjin Li (4172617), Xiongwei Nie (9321744), Guiqing Peng (90055), Zhelin Su (11797970), Changzhi Zhao (1647001), Yanan Fu (4422967), Yuan Zhou (179597), Shengsong Xie (582082), Yubei Tan (11797973), Shuhong Zhao (48168), Yangyang Li (554659), Zhen Fu (669777), Yixin Xiang (11797976), Fei Liu (21082)   +15 more
core   +1 more source

MiniSdd7‐Derived Cytosine Base Editor Promotes Efficient Multiplex Gene Knockout in Porcine Primary Fibroblasts

Animal Research and One Health, EarlyView.
The miniSdd7‐BE4max‐SpG system enables highly efficient multiplex C‐to‐T base editing of APN, CD163, and MSTN in porcine fibroblasts, providing a robust platform for future development of multiplex gene‐edited livestock. ABSTRACT Efficient multiplex genome editing is essential for improving complex traits in livestock that are governed by multiple ...
Shaoshuai Wang, Yu Wang, Dengfeng Bi, Jianguo Zhao, Yanfang Wang   +4 more
wiley   +1 more source

Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein

Viruses, 2016
The coronavirus membrane (M) protein acts as a dominant immunogen and is a major player in virus assembly. In this study, we prepared two monoclonal antibodies (mAbs; 1C3 and 4C7) directed against the transmissible gastroenteritis virus (TGEV) M protein.
Hui Dong, Xin Zhang, Hongyan Shi, Jianfei Chen, Da Shi, Yunnuan Zhu, Li Feng   +6 more
doaj   +1 more source

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

POLDIP3 deletion promoted the replications of PEDV and TGEV.

, 2023
WT and POLDIP3-/- cells were infected with PEDV and TGEV for 24 h, respectively, and then the samples were analyzed for detection of N mRNA by RT-qPCR. (TIF)
Jianfei Chen (3186999), Jin Tian (158712), Haoxin Yan (17108715), Mingwei Li (1914862), Longjun Guo (315268), Yudi Pan (7309513), Hongyan Shi (1988656), Da Shi (1455439), Li Feng (77923), Yang Wu (66682)   +9 more
core   +1 more source

THE CO-ADMINISTRATION OF POIL-2 WITH TGEV INACTIVATED VACCINE ENHANCES IMMUNE RESPONSE OF PIGLETS TO TGEV

The Journal of Animal and Plant Sciences
Porcine interleukin-2 (poIL-2) has not yet been demonstrated to be immune-enhancing against porcine transmissible gastroenteritis virus (TGEV) inactivated vaccine (IV), despite IL-2 having been proven to have immunological adjuvant effects for a variety of vaccinations. In this work, the impact of poIL-2 on TGEV IV in terms of immunological enhancement
H.Y. Yu, D.M. Gao, F.H. Li, D.P. Peng, B.B. Xia, Z.Y. He, W.J. Xu, H.Z. Li, J Du, J Zhao   +9 more
openaire   +1 more source