Results 261 to 270 of about 574,833 (290)

Nanopore Third-Generation Sequencing for Comprehensive Analysis of Hemoglobinopathy Variants

Clinical Chemistry, 2023
Abstract Background Oxford Nanopore Technology (ONT) third-generation sequencing (TGS) is a versatile genetic diagnostic platform. However, it is nonetheless challenging to prepare long-template libraries for long-read TGS, particularly the ONT method for analysis of hemoglobinopathy variants ...
Weilun Huang, Shoufang Qu, Qiongzhen Qin, Xu Yang, Wanqing Han, Yongli Lai, Jiaqi Chen, Shihao Zhou, Xuexi Yang, Wanjun Zhou   +9 more
openaire   +2 more sources

Enhancing Thalassemia Diagnosis: Advantages of Third-Generation Sequencing

Clinical Laboratory
This study aimed to evaluate the efficacy of third-generation sequencing (TGS) and a thalassemia (Thal) gene diagnostic kit in identifying Thal gene mutations.Blood samples (n = 119) with positive hematology screening results were tested using polymerase chain reaction (PCR)-based methods and TGS on the PacBio-Sequel-II-platform, respectively.Out of ...
Minjun, Huang, Jiexiang, Huang, Liumin, Yu, Kun, Lin   +3 more
openaire   +2 more sources

Third Generation DNA Sequencing with a Nanopore

2011
With the advent of Next-Generation-Sequencing (NGS) technologies, an enormous volume of DNA sequencing data can be generated at low cost, placing genomic science within the grasp of everyday medicine. However, mired in this voluminous data, a new problem has emerged: the assembly of the genome from the short reads.
Gregory Timp, Utkur Mirsaidov, Winston Timp, Jiwook Shim, Deqiang Wang, Valentin Dimitrov, Jan Scrimgeour, Chunchen Lin, Jeffrey Comer, Anthony H. Ho, Xueqing Zou, Aleksei Aksimentiev, Klaus Schulten   +12 more
openaire   +1 more source

Next-Generation and Third-Generation Sequencing of Lung Cancer Biomarkers

2017
In lung cancer, biomarker-guided therapy selection has greatly improved patient outcome. The recent introduction of next-generation sequencing (NGS) technology has rapidly expanded our understanding of disease-driving gene mutations and resulted in identification of key lung cancer biomarkers.
openaire   +1 more source

Third-generation sequencing fireworks at Marco Island

Nature Biotechnology, 2010
David J Munroe, Timothy J R Harris
openaire   +1 more source

Clinical Metagenomic Next-Generation Sequencing for Pathogen Detection

Annual Review of Pathology: Mechanisms of Disease, 2019
Wei Gu, Steve Miller, Charles Y Chiu
exaly  

The role of replicates for error mitigation in next-generation sequencing

Nature Reviews Genetics, 2013
Kimberly Robasky, Nathan E Lewis, George M Church   +2 more
exaly  

Advances in understanding cancer genomes through second-generation sequencing

Nature Reviews Genetics, 2010
Matthew L Meyerson, Stacey Gabriel, Gad Getz   +2 more
exaly  

Coming of age: ten years of next-generation sequencing technologies

Nature Reviews Genetics, 2016
Sara Goodwin, John D Mcpherson, W Richard Mccombie   +2 more
exaly  

Genome-wide genetic marker discovery and genotyping using next-generation sequencing

Nature Reviews Genetics, 2011
John W Davey, Jason Q Boone, Julian M Catchen   +2 more
exaly