Results 31 to 40 of about 18,631,052 (336)

Three dimensional microcarrier system in mesenchymal stem cell culture: a systematic review

open access: yesCell & Bioscience, 2020
Stem cell-based regenerative medicine is a promising approach for tissue reconstruction. However, a large number of cells are needed in a typical clinical study, where conventional monolayer cultures might pose a limitation for scale-up.
Benson Koh   +6 more
doaj   +1 more source

Application of Prostate Cancer Models for Preclinical Study: Advantages and Limitations of Cell Lines, Patient-Derived Xenografts, and Three-Dimensional Culture of Patient-Derived Cells

open access: yesCells, 2019
Various preclinical models have been developed to clarify the pathophysiology of prostate cancer (PCa). Traditional PCa cell lines from clinical metastatic lesions, as exemplified by DU-145, PC-3, and LNCaP cells, are useful tools to define mechanisms ...
Takeshi Namekawa   +3 more
semanticscholar   +1 more source

A Model of Lymphangioleiomyomatosis in a Three-Dimensional Culture System [PDF]

open access: yesLymphatic Research and Biology, 2015
Lymphangioleiomyomatosis (LAM) is a tumor consisting of benign-looking neoplastic cells, but its wretched clinical outcome often resembles a malignant disease. LAM cell clusters (LCCs), unique microstructures commonly found in LAM-associated chylous effusion, are aggregates of LAM cells rimmed by lymphatic endothelium.
Toshio, Kumasaka   +7 more
openaire   +2 more sources

Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers

open access: yesRegenerative Therapy, 2020
Clinically relevant human induced pluripotent stem cell (hiPSC) derivatives require efficient protocols to differentiate hiPSCs into specific lineages.
Denise Zujur   +15 more
doaj   +1 more source

Modification of three-dimensional transition in the wake of a rotationally oscillating cylinder [PDF]

open access: yes, 2010
A study of the flow past an oscillatory rotating cylinder has been conducted, where the frequency of oscillation has been matched to the natural frequency of the vortex street generated in the wake of a stationary cylinder, at Reynolds number 300.
Leontini, Justin S.   +7 more
core   +1 more source

The study of establishment of an in vivo tumor model by three-dimensional cells culture systems methods and evaluation of antitumor effect of biotin-conjugated pullulan acetate nanoparticles

open access: yesArtificial Cells, Nanomedicine, and Biotechnology, 2019
In this study, three-dimensional (3D) hydrogels were used for human hepatocellular carcinoma (HepG2) cells culture systems in vitro and establishment of an in vivo xenografted tumor model.
Hongli Chen   +7 more
doaj   +1 more source

Mxi1 influences cyst formation in three-dimensional cell culture [PDF]

open access: yesBMB Reports, 2012
Cyst formation is a major characteristic of ADPKD and iscaused by the abnormal proliferation of epithelial cells. Renalcyst formation disrupts renal function and induces diversecomplications.
Yeon Joo Yook   +9 more
doaj   +1 more source

Three Dimensional Culture of Potential Epithelial Progenitor Cells in Human Lacrimal Gland

open access: yesTranslational Vision Science & Technology, 2019
Purpose We investigate human lacrimal gland tissue to determine the presence of progenitor cells in this adult human tissue. Methods Six human lacrimal gland tissues from donors were collected and stored immediately in the culture medium at 4°C until the
Hui Lin, Ying Liu, S. Yiu
semanticscholar   +1 more source

Three-Dimensional Organotypic Culture of Stratified Epithelia [PDF]

open access: yesCold Spring Harbor Protocols, 2015
One of the limitations of conventional tissue culture on flat two-dimensional surfaces is the loss of complex interactions between the epithelium and stroma. We have devised a culture system that recreates the salient features of the stratified epithelium using primary cell cultures from mouse models.
Andrew D, Rhim, Anil K, Rustgi
openaire   +2 more sources

Development of a novel and economical agar-based non-adherent three-dimensional culture method for enrichment of cancer stem-like cells

open access: yesStem cell research & therapeutics, 2018
Non-adherent or ultra-low attachment three-dimensional (3D) culture, also called sphere formation assay, has been widely used to assess the malignant phenotype and stemness potential of transformed or cancer cells.
Weijie Gao   +8 more
semanticscholar   +1 more source

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