Assessment of Thymidine Phosphorylase Function: Measurement of Plasma Thymidine (and Deoxyuridine) and Thymidine Phosphorylase Activity [PDF]
, 2011 We describe detailed methods to measure thymidine (dThd) and deoxyuridine (dUrd) concentrations and thymidine phosphorylase (TP) activity in biological samples. These protocols allow the detection of TP dysfunction in patients with mitochondrial neurogastrointestinal encephalomyopathy (MNGIE).
Ramon Martí +3 more
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Retrospective natural history of thymidine kinase 2 deficiency
Journal of Medical Genetics, 2018 Background Thymine kinase 2 (TK2) is a mitochondrial matrix protein encoded in nuclear DNA and phosphorylates the pyrimidine nucleosides: thymidine and deoxycytidine.
C. Garone +42 more
semanticscholar +1 more source
Environmental Research Letters, 2013 We compared planktonic primary and secondary production across twenty meltwater ponds on the surface of the McMurdo Ice Shelf in January 2007, including some ponds with basal brines created by meromictic stratification.
Brian K Sorrell, Ian Hawes, Karl Safi
doaj +1 more source
Unchanged thymidine triphosphate pools and thymidine metabolism in two lines of thymidine kinase 2‐mutated fibroblasts [PDF]
The FEBS Journal, 2009 Mitochondrial thymidine kinase (TK2) catalyzes the phosphorylation of thymidine in mitochondria. Its function becomes essential for dTTP synthesis in noncycling cells, where cytosolic dTTP synthesis via R1/R2 ribonucleotide reductase and thymidine kinase 1 is turned down.
FRANGINI, MIRIAM +6 more
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Accumulation of thymidine-derived sugars in thymidine phosphorylase overexpressing cells [PDF]
Biochemical Pharmacology, 2010 Thymidine phosphorylase (TP) is often overexpressed in cancer and potentially plays a role in the stimulation of angiogenesis. The exact mechanism of angiogenesis induction is unclear, but is postulated to be related to thymidine-derived sugars. TP catalyzes the conversion of thymidine (TdR) to thymine and deoxyribose-1-phosphate (dR-1-P), which can be
Bijnsdorp, I.V. +8 more
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Thymidine Catabolism as a Metabolic Strategy for Cancer Survival
Cell Reports, 2017 Thymidine phosphorylase (TP), a rate-limiting enzyme in thymidine catabolism, plays a pivotal role in tumor progression; however, the mechanisms underlying this role are not fully understood.
Sho Tabata +20 more
doaj +1 more source
Fission yeast strains with circular chromosomes require the 9-1-1 checkpoint complex for the viability in response to the anti-cancer drug 5-fluorodeoxyuridine. [PDF]
PLoS ONE, 2017 Thymidine kinase converts 5-fluorodeoxyuridine to 5-fluorodeoxyuridine monophosphate, which causes disruption of deoxynucleotide triphosphate ratios.
Hossain Mohammad Shamim +5 more
doaj +1 more source
In vivo labeling of fission yeast DNA with thymidine and thymidine analogs [PDF]
Methods, 2004 In vivo labeling of DNA with thymidine and thymidine analogs has long been a cornerstone of replication studies. Unfortunately, yeast lack a thymidine salvage pathway and thus do not incorporate exogenous thymidine. Specifically, yeast neither efficiently take up exogenous thymidine from their growth media nor phosphorylate it to thymidylate, the ...
Sivakumar, Sasirekha +4 more
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PLoS ONE, 2021 Adult growth hormone deficiency (GHD), a condition characterized by increased oxidative stress, is related to augmented cardiovascular, metabolic and oncological risk. A case-control observational study has been performed to evaluate DNA oxidative damage
Antonio Mancini +8 more
doaj +1 more source
Optimisation of the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss)
Veterinární Medicína, 2019 The lymphocyte proliferation assay is a valuable method used for the evaluation of the fish immune system. However, there are many variations and optimal results are not always obtained.
Hana Minarova +6 more
doaj +1 more source