Results 71 to 80 of about 199,261 (304)

Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments

Biotechnology Progress, EarlyView.
Abstract The quality control of RNA has become increasingly crucial with the rise of mRNA‐based vaccines and therapeutics. However, conventional methods such as LC–MS often require specialized equipment and expertise, limiting their applicability to high throughput experiments.
Rochelle Aw, Karen Polizzi
wiley   +1 more source

Utilization of exogenous thymidine by Chlamydia psittaci growing in the thymidine kinase-containing and thymidine kinase-deficient L cells [PDF]

Journal of Bacteriology, 1976
The incorporation of [3H]thymidine into the deoxyribonucleic acid (DNA) of Chlamydia psittaci (strain 6BC) growing in thymidine kinase (adenosine 5'-triphosphate-thymidine 5'-phosphotransferase, EC 1.7.1.21)-containing L cells, L(TK+), and thymidine kinase-deficient L cells, LM(TK-), was examined by autoradiography. Label was detected over C.
openaire   +3 more sources

Thymidine Phosphorylase Inhibitory Potential and Molecular Docking Studies of Secondary Metabolites Isolated From Fernandoa adenophylla (Wall. ex G. Don) Steenis

Chemistry &Biodiversity, EarlyView.
ABSTRACT This study investigates the potential of Fernandoa adenophylla, a South American plant, as a reservoir of compounds with thymidine phosphorylase (TP) inhibitory activity. Through a comprehensive approach combining in vitro assays and molecular docking analysis, we isolated and characterized bioactive compounds from F.
Abdur Rauf, Majid Khan, Umer Rashid, Anees Saeed, Zafar Ali Shah, Zubair Ahmad, Abdulhakeem S. Alamri, Walaa F. Alsanie, Imtiaz Khan, Humaira Hussain, Majid Alhomrani, Marcello Iriti   +11 more
wiley   +1 more source

TYMP (thymidine phosphorylase) [PDF]

Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2011
Review on TYMP (thymidine phosphorylase), with data on DNA, on the protein encoded, and where the gene is implicated.
Bijnsdorp, IV, Peters, GJ
openaire   +3 more sources

Synthesis and Metal‐Ion Binding Properties of Duplexes Containing Thymine Analogs with 1,2‐Diamine Groups

ChemBioChem, Volume 26, Issue 11, June 3, 2025.
The findings of thermal denaturation experiments indicate that duplexes containing thymine analogues with 1,2‐ethylenediamine side chains show stabilizing effects with five metal ions (e.g., Cu(II), Ni(II), Zn(II), Co(II), Cd(II)). The 1,2‐diamine side chain demonstrated to be useful as a new metal‐ion binding site.
Takahiro Atsugi, Shoji Fujiwara, Jiro Kondo, Akira Ono   +3 more
wiley   +1 more source

Purine Chemistry in the Early RNA World at the Origins of Life: From RNA and Nucleobases Lesions to Current Key Metabolic Routes

ChemBioChem, Volume 26, Issue 11, June 3, 2025.
In the nascent processes of the beginnings and evolution of life, nucleobases and especially purines, ribonucleos(t)ides and primitive RNAs have been continuously modified. A RNA‐peptide world and key metabolic pathways probably have emerged from the corresponding chemical modifications resulting from adenine deamination, purine alkylation and ...
Jean‐Luc Décout, Marie‐Christine Maurel   +1 more
wiley   +1 more source

Human Keratinocytes Catabolize Thymidine

Journal of Investigative Dermatology, 1988
Human neonatal foreskin keratinocytes incorporate exogenous thymidine into DNA and proliferate in vitro even after reaching confluence. Keratinocytes also catabolize thymidine, as reported for the first time below. Stratified cultures of keratinocytes reduced the amount of thymidine in the medium by more than 90% within 2 to 4 h. Consequently, the rate
Lynne M. Hough, Lynne M. Hough, Leonard M. Milstone, Leonard M. Milstone, Pauline M. Schwartz, Pauline M. Schwartz, Lisa C. Kugelman, Lisa C. Kugelman, Yael. Coifman, Yael. Coifman   +9 more
openaire   +3 more sources

Unbalanced deoxynucleotide pools cause mitochondrial DNA instability in thymidine phosphorylase-deficient mice.

Human Molecular Genetics, 2009
Replication and repair of DNA require equilibrated pools of deoxynucleoside triphosphate precursors. This concept has been proven by in vitro studies over many years, but in vivo models are required to demonstrate its relevance to multicellular organisms
L. Lopez, H. Akman, Á. García-Cazorla, B. Dorado, R. Martí, I. Nishino, S. Tadesse, G. Pizzorno, D. Shungu, E. Bonilla, K. Tanji, M. Hirano   +11 more
semanticscholar   +1 more source

Light‐Responsive Mononucleotide Coacervates

Chemistry – A European Journal, Volume 31, Issue 31, June 3, 2025.
Light‐responsive mononucleotide coacervates undergo reversible phase transitions via azobenzene photoisomerization. Coacervates with high‐valency azobenzenes remain stable under UV light, while those with low‐valency azobenzenes dissolve, enabling nucleotide release. This minimal system highlights the potential role of light for exploring energy‐driven
Edison Rafael Jimenez Granda, Hedi Karoui, Xavier Brilland, Jean‐Christophe Baret, Nicolas Martin   +4 more
wiley   +1 more source

The effect of fluoropyrimidines with or without thymidine phosphorylase inhibitor on the expression of thymidine phosphorylase

European Journal of Pharmacology, 2004
Thymidine phosphorylase (platelet-derived-endothelial-cell-growth-factor) catalyzes the reversible phosphorolysis of thymidine to thymine and 2-deoxyribose-1-phosphate, activates 5'-deoxy-5-fluorouridine (5'DFUR) and inactivates trifluorothymidine (TFT).
de Bruin, M., van Capel, T, Smid, K., Fukushima, M, Hoekman, K., Pinedo, H.M., Peters, G.J.   +6 more
openaire   +4 more sources