Results 81 to 90 of about 127,095 (220)

Whole tissue imaging of cellular boundaries at sub‐micron resolutions for deep learning cell segmentation: Applications in the analysis of epithelial bending of ectoderm

Developmental Dynamics, EarlyView.
Abstract Background To understand cellular morphology, biologists have relied on traditional optical microscopy of tissues combined with tissue clearing protocols to image structures deep within tissues. Unfortunately, these protocols often struggle to retain cell boundary markers, especially at high enough resolutions necessary for precise cell ...
Sam C. P. Norris, Jimmy K. Hu, Neil H. Shubin   +2 more
wiley   +1 more source

Biophysical processes of morphogenesis in lizard lungs

Developmental Dynamics, EarlyView.
Abstract Background The lungs of squamate reptiles (lizards and snakes) are highly diverse, exhibiting single chambers, multiple chambers, transitional forms with two to three chambers, along with a suite of other anatomical features, including finger‐like epithelial projections into the body cavity known as diverticulae.
Kaleb Hill, Aaron H. Griffing, Michael A. Palmer, Bezia Lemma, Aria S. Lupo, Tony Gamble, Natalia A. Shylo, Andrej Košmrlj, Paul A. Trainor, Celeste M. Nelson   +9 more
wiley   +1 more source

Labeling DNA with Stable Isotopes: Economical and Practical Considerations

BioTechniques, 2001
Labeling DNA with stable isotopes to measure cell proliferation can be a technique as effective as 3H-thymidine labeling without the limitations imposed by using radioisotopes.
G.E. Black, Y.C. Boller, K.A. Kennedy, P. Lecchi, F.P. Abramson   +4 more
doaj   +1 more source

The In Vitro Transgenic Rodent Assay in Primary MutaMouse Hepatocytes Compared to the Mammalian Cell Gene Mutation Assay Using the HPRT Gene

Environmental and Molecular Mutagenesis, EarlyView.
ABSTRACT Gene mutations can be detected in mammalian cells in vitro using indicator genes such as the hypoxanthine‐guanine‐phosphoribosyltransferase (HPRT) gene. These assays have been adopted as OECD test guidelines (TG, e.g., OECD TG no. 476) and are used for regulatory purposes.
Alina Göpfert, Kylee Kendra Marie Ronnenberg, Claudia Ruelker, Silke Spang, Naveed Honarvar, Robert Landsiedel   +5 more
wiley   +1 more source

EdU labeling of Trypanosome Cells and Their Kinetoplast DNA (kDNA)

Bio-Protocol, 2013
Trypanosome mitochondrial genome, known as Kinetoplast DNA (kDNA), is a massive network of interlocked DNA rings. The studies of kDNA replication and architecture are of major significance since kDNA is a valid drug target.
Jianyang Wang
doaj   +1 more source

Guidelines for Light‐Start One‐Pot RPA‐CRISPR‐Cas12a Assays for Foodborne Pathogen Detection

Food Safety and Health, EarlyView.
Light‐start one‐pot RPA‐CRISPR‐Cas12a detection schematic diagram. (A) Linear crRNA activation light‐start one‐pot workflow. (B) Caged crRNA activation light‐start one‐pot workflow. ABSTRACT Rapid and accurate detection of nucleic acids of foodborne pathogens is of vital importance for controlling the spread of foodborne diseases and safeguarding ...
Fan Li, Ruiting Yao, Xinxin Jiang, Haorui Zhu, Guoliang Li   +4 more
wiley   +1 more source

Thymidine Phosphorylase/β-tubulin III expressions predict the response in Chinese advanced gastric cancer patients receiving first-line capecitabine plus paclitaxel

BMC Cancer, 2011
Background To assess the role of Thymidine Phosphorylase and β-tubulin III in clinical outcome of Chinese advanced gastric cancer patients receiving first-line capecitabine plus paclitaxel.
Shen Lin, Li Yan-yan, Yu Jing-wei, Lu Ming, Gao Jing   +4 more
doaj   +1 more source

Somatic cell reprogramming for Parkinson's disease treatment

Ibrain, Volume 11, Issue 1, Page 59-73, Spring 2025.
The fundamental purpose of cell reprogramming to treat Parkinson's disease is to generate dopaminergic neurons (DAN) and do transplantation. There are two ways to accomplish this. One method is to induce cells into induced DA neurons (iDAN) directly or to induce cells into induced pluripotent stem cells and ultimately into iDAN in vitro. Another option
Xiaozhuo Li, Kevin Fang, Fengping Wang
wiley   +1 more source

Noncanonical Nucleotides in the Genome Around the Maternal‐Zygotic Transition

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
In this paper, Kazzazi et al. provide a comprehensive review of the dynamics of nonconventional nucleotides in the genome during early developmental stages, hypothesizing a potential role for these nucleotides in the activation of the zygotic genome. ABSTRACT From the very moment of fertilization and throughout development, the cells of animal embryos ...
Latifa Kazzazy, Dávid Mező, Kinga K. Nagy, Viktória Perey‐Simon, Judit Tóth, Angéla Békési, Beáta Vértessy, Máté Varga   +7 more
wiley   +1 more source

Identification of Natural and Artificial Modifications in DNA at the Nanometer Level

Journal of Raman Spectroscopy, EarlyView.
Short single‐ and double‐stranded DNA sequences with natural and artificial markers are examined at ambient conditions using TERS to identify and localize the labelled nucleobases. The differences between single‐ and double‐stranded DNA should provide clear experimental evidence of the extreme surface sensitivity of TERS.
Tanja Deckert‐Gaudig, Prabha Singh, Miklos Veres, Roman Holomb, Volker Deckert   +4 more
wiley   +1 more source