Results 61 to 70 of about 62,555 (239)

An Adenovirus Vector Containing the Suicide Gene Thymidine Kinase for a Broad Application in Cancer Gene Therapy

Memorias do Instituto Oswaldo Cruz, 2002
Treatment of cancer using gene therapy is based on adding a property to the cell leading to its elimination. One possibility is the use of suicide genes that code for enzymes that transform a pro-drug into a cytotoxic product.
GS Magalhães, AR Muotri, MCN Marchetto, CFM Menck, AM Ventura   +4 more
doaj   +1 more source

Circulating Cell‐Free DNA of Bovine Leukemia Virus: A Promising Biomarker for Enzootic Bovine Leukosis

Microbiology and Immunology, EarlyView.
ABSTRACT Bovine leukemia virus (BLV) causes Enzootic bovine leukosis (EBL) in approximately 1%–5% of infected cattle after a long latent period. Few biomarkers effectively distinguish non‐EBL from EBL cattle. Given the rapid turnover of tumor cells, we hypothesized that cell‐free DNA (cfDNA) in plasma could serve as a more effective biomarker for EBL ...
M. Ishrat Jahan, Toshiaki Inenaga, Sakurako Makimoto, Md. Belal Hossain, Yuka Matsuoka, Sharmin Nahar Sithi, Samiul Alam Rajib, Arif Nur Muhammad Ansori, Kenji Sugata, Kazuhiko Imakawa, Tomoko Kobayashi, Yorifumi Satou   +11 more
wiley   +1 more source

Cross‐Analysis of Single‐Cell Transcriptomic Datasets Reveals Conserved Neurogenic Gene Signatures and New Insights Into Neural Stem Cell Aging

Aging Cell, EarlyView.
Here, we reanalyze single‐cell RNA sequencing datasets demonstrating hippocampal adult neural stem cell (NSC) heterogeneity to resolve classification inconsistencies and identify conserved gene markers. Our findings enhance understanding of NSC dynamics, aging, and senescence, providing a framework for standardized classification and improved insights ...
Oliver Polzer, E. Kinloch, P. J. Lucassen, E. Salta, C. P. Fitzsimons   +4 more
wiley   +1 more source

Tissue‐Specific Effects of Dietary Protein on Cellular Senescence Are Mediated by Branched‐Chain Amino Acids

Aging Cell, EarlyView.
Restriction of dietary BCAAs protects male mice from the metabolic consequences of both normal‐ and high‐protein diets. BCAA restriction also protects from hepatic cellular senescence in vivo and in vitro, especially in the context of mitochondrial stress. ABSTRACT Dietary protein is a key regulator of healthy aging in both mice and humans.
Mariah F. Calubag, Ismail Ademi, Isaac D. Grunow, Lucia E. Breuer, Reji Babygirija, Penelope Lialios, Sandra M. Le, Michelle M. Sonsalla, Julia A. Illiano, Bailey A. Knopf, Fan Xiao, Dennis Minton, Adam R. Konopka, David A. Harris, Dudley W. Lamming   +14 more
wiley   +1 more source

Concentration of human thymidine kinase 1 discover invisible malignant human tumours

European Journal of Cell Biology, 2022
Early discover of risk progression of invisible carcinomas is important for a prerequisite successful treatment. Here, we investigated whether concentration of human thymidine kinase 1 (HTK1) discover invisible malignant human tumours.
Ji Zhou, Huijun Li, Cong Fang, Peng Gao, Cuicui Jin, Sonbo Liu, Rougu Zou, Jin Li, Yougping Liu, Ellen He, Sven Skog   +10 more
doaj  

Alpha-Herpesvirus Thymidine Kinase Genes Mediate Viral Virulence and Are Potential Therapeutic Targets

Frontiers in Microbiology, 2019
Alpha-herpesvirus thymidine kinase (TK) genes are virulence-related genes and are nonessential for viral replication; they are often preferred target genes for the construction of gene-deleted attenuated vaccines and genetically engineered vectors for ...
Ying Xie, Ying Xie, Ying Xie, Liping Wu, Liping Wu, Liping Wu, Mingshu Wang, Mingshu Wang, Mingshu Wang, Anchun Cheng, Anchun Cheng, Anchun Cheng, Qiao Yang, Qiao Yang, Qiao Yang, Ying Wu, Ying Wu, Ying Wu, Renyong Jia, Renyong Jia, Renyong Jia, Dekang Zhu, Dekang Zhu, XinXin Zhao, XinXin Zhao, XinXin Zhao, Shun Chen, Shun Chen, Shun Chen, Mafeng Liu, Mafeng Liu, Mafeng Liu, Shaqiu Zhang, Shaqiu Zhang, Shaqiu Zhang, Yin Wang, Zhiwen Xu, Zhengli Chen, Ling Zhu, Qihui Luo, Yunya Liu, Yunya Liu, Yunya Liu, Yanling Yu, Yanling Yu, Yanling Yu, Ling Zhang, Ling Zhang, Ling Zhang, Xiaoyue Chen, Xiaoyue Chen   +50 more
doaj   +1 more source

Imatinib decreases germ cell survival and germline stem cell proliferation in rodent testis ex vivo and in vitro

Andrology, EarlyView.
Abstract Background Imatinib and dasatinib are tyrosine kinase inhibitors (TKIs) increasingly used to treat several diseases in both children and adults at fertile age. We have previously shown that imatinib has adverse effects on developing testis, and imatinib‐treated male patients have been reported to have reduced sperm counts.
Anna Eggert, Sini Laasanen, Mirja Nurmio, Aida Wahlgren, Kirsi Jahnukainen, Kim Eerola, Miisael Nieminen, Opeyemi Olotu, Noora Kotaja, Juho‐Antti Mäkelä, Jorma Toppari   +10 more
wiley   +1 more source

Mouse genome engineering uncovers 18 genes dispensable for male reproduction

Andrology, EarlyView.
Abstract Background Male infertility is an intricate multifactorial disease involving the interplay between genetic and environmental factors. Genetic anomalies account for more than 15% of all male infertility cases; however, diagnosing them exhibits enormous challenges due to variable symptomatic presentations and limited knowledge of gene functions.
Hsin‐Yi Chang, Yonggang Lu, Kaito Yamamoto, Jiang Sun, Keisuke Shimada, Yuki Hiradate, Yoshitaka Fujihara, Masahito Ikawa   +7 more
wiley   +1 more source

A comparative study of replicative properties of antitumor recombinant vaccinia viruses on human glioblastoma cell culture U87 and monkey kidney cell culture CV-1

Вавиловский журнал генетики и селекции, 2017
In the world of today, virotherapy is one of the rapidly developing areas in the treatment of cancer, and its advantage is selective destruction of cancer cells with minimizing the destructive effect on normal cells of the body. A promising basis for the
R. A. Maksyutov, I. V. Kolosova, T. V. Tregubchak, I. A. Razumov, S. N. Shchelkunov   +4 more
doaj   +1 more source

Maize Thymidine Kinase Activity Is Present throughout Plant Development and Its Heterologous Expression Confers Tolerance to an Organellar DNA-Damaging Agent

Plants, 2020
Thymidine kinase 1 (TK1) phosphorylates thymidine nucleosides to generate thymidine monophosphate. This reaction belongs to the pyrimidine salvage route that is phylogenetically conserved.
Manuela Nájera-Martínez, José Antonio Pedroza-García, Luis Jiro Suzuri-Hernández, Christelle Mazubert, Jeannine Drouin-Wahbi, Jorge Vázquez-Ramos, Cécile Raynaud, Javier Plasencia   +7 more
doaj   +1 more source