Results 51 to 60 of about 134,958 (300)

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

5 ps Jitter Programmable Time Interval/Frequency Generator

Metrology and Measurement Systems, 2017
A new time interval/frequency generator with a jitter below 5 ps is described. The time interval generation mechanism is based on a phase shifting method with the use of a precise DDS synthesizer. The output pulses are produced in a Spartan-6 FPGA device,
Kwiatkowski Paweł, Różyc Krzysztof, Sawicki Marek, Jachna Zbigniew, Szplet Ryszard   +4 more
doaj   +1 more source

A 19 ps Precision and 170 M Samples/s Time-to-Digital Converter Implemented in FPGA with Online Calibration

Applied Sciences, 2022
This paper presents a 19 ps precision and 170 M samples/s time-to-digital converter (TDC) in FPGA. Through the direct count method and tapped delay line method, the coarse count and fine count can be extracted, respectively.
Mengdi Zhang, Ye Zhao, Zhengsheng Han, Fazhan Zhao   +3 more
doaj   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

A novel FPGA-based time-to-digital converter featuring machine learning-aided self-calibration

Intelligent Systems with Applications
Time-to-digital converters (TDCs) implemented on Field-Programmable Gate Arrays (FPGAs) encounter significant challenges in achieving high precision. Unlike Application-Specific Integrated Circuits, FPGA-based TDCs must depend on standard logic elements ...
Arash Amini Bardpareh, Eleonora Vacca, Davide Nicolini, Corrado De Sio, Sarah Azimi, Luca Sterpone, Elisa Fiorina, Emanuele Maria Data, Felix Mas Milian   +8 more
doaj   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

A 0.002‐mm2 8‐bit 1‐MS/s low‐power time‐based DAC (T‐DAC)

IET Circuits, Devices and Systems, 2021
Digital‐to‐analogue converters (DACs) are essential blocks for interfacing the digital environment with the real world. A novel architecture, using a digital‐to‐time converter (DTC) and a time‐to‐voltage converter (TVC), is employed to form a low‐power ...
Ali H. Hassan, Hassan Mostafa, Mohamed Refky, Khaled N. Salama, Ahmed M. Soliman   +4 more
doaj   +1 more source