Results 71 to 80 of about 209,245 (296)

Structure-guided design of a potent Clostridioides difficile toxin A inhibitor

Frontiers in Microbiology, 2023
Crystal structures of camelid heavy-chain antibody variable domains (VHHs) bound to fragments of the combined repetitive oligopeptides domain of Clostridioides difficile toxin A (TcdA) reveal that the C-terminus of VHH A20 was located 30 Å away from the ...
Greg Hussack, Martin A. Rossotti, Henk van Faassen, Tomohiko Murase, Luiz Eugenio, Joseph D. Schrag, Kenneth K.-S. Ng, Kenneth K.-S. Ng, Jamshid Tanha, Jamshid Tanha   +9 more
doaj   +1 more source

Dominant-negative Inhibitors of the Clostridium perfringens ϵ-Toxin [PDF]

Journal of Biological Chemistry, 2009
The Clostridium perfringens epsilon-toxin is responsible for a severe, often lethal intoxication. In this study, we characterized dominant-negative inhibitors of the epsilon-toxin. Site-specific mutations were introduced into the gene encoding epsilon-toxin, and recombinant proteins were expressed in Escherichia coli. Paired cysteine substitutions were
Teal M, Pelish, Mark S, McClain
openaire   +2 more sources

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

The relationship between the metabolism of sphingomyelin species and the hemolysis of sheep erythrocytes induced by Clostridium perfringens α-toxins⃞

Journal of Lipid Research, 2008
Clostridium perfringens α-toxin induces the hemolysis of sheep erythrocytes by activating the metabolism of sphingomyelin (SM) via a GTP binding protein in membranes.
Masataka Oda, Takayuki Matsuno, Ryouta Shiihara, Sadayuki Ochi, Rieko Yamauchi, Yuki Saito, Hiroshi Imagawa, Masahiro Nagahama, Mugio Nishizawa, Jun Sakurai   +9 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Small molecule inhibitors of anthrax lethal factor toxin [PDF]

Bioorganic & Medicinal Chemistry, 2014
This manuscript describes the preparation of new small molecule inhibitors of Bacillus anthracis lethal factor. Our starting point was the symmetrical, bis-quinolinyl compound 1 (NSC 12155). Optimization of one half of this molecule led to new LF inhibitors that were desymmetrized to afford more drug-like compounds.
John D, Williams, Atiyya R, Khan, Steven C, Cardinale, Michelle M, Butler, Terry L, Bowlin, Norton P, Peet   +5 more
openaire   +2 more sources

PARK(ing) time–How park deficiency affects the biological clock in a Drosophila model of Parkinson's disease

FEBS Letters, EarlyView.
Drosophila park mutants serve as a model for Parkinson's disease. We used this strain to investigate the connection between oxidative stress and the circadian clock mechanism. We showed that increased oxidative stress affects the physiology of pacemaker cells, disrupting their daily structural plasticity. Lack of rhythmic signaling from pacemaker cells
Kamila Zientara, Kinga Skoczek, Elzbieta Pyza, Milena Damulewicz   +3 more
wiley   +1 more source

α‐Synuclein aggregation landscape from phase separation to neurotoxic intermediates

FEBS Letters, EarlyView.
Alpha‐synuclein aggregation in Parkinson's disease involves a complex landscape of transient intermediates, including oligomers, fibrils and liquid–liquid phase separation (LLPS). A view is emerging in which LLPS maturation into solid‐like condensates may contribute to the formation of neurotoxic species.
Silvia Arino, Giuliana Fusco, Alfonso De Simone   +2 more
wiley   +1 more source

Heterobifunctional Multivalent Inhibitor-Adaptor Mediates Specific Aggregation between Shiga Toxin and a Pentraxin

, 2016
The first example of a multivalent heterofunctional inhibitor-adaptor, called “BAIT”, is described. This multivalent inhibitor-adaptor is able to capture a “target” receptor (Shiga toxin) through its recognition of one ligand of a heterobivalent ...
David R. Bundle (169253), Pavel I. Kitov (1438465), Eugenia Paszkiewicz (1820272), Dmitry Solomon (2739202), Gordon A. Grant (2739199), Joanna M. Sadowska (1820281)   +5 more
core   +1 more source

Polyvalent inhibitors of anthrax toxin that target host receptors [PDF]

Proceedings of the National Academy of Sciences, 2006
Resistance of pathogens to antimicrobial therapeutics has become a widespread problem. Resistance can emerge naturally, but it can also be engineered intentionally, which is an important consideration in designing therapeutics for bioterrorism agents. Blocking host receptors used by pathogens represents a powerful strategy to overcome this
Saleem, Basha, Prakash, Rai, Vincent, Poon, Arundhati, Saraph, Kunal, Gujraty, Mandy Y, Go, Skanda, Sadacharan, Mia, Frost, Jeremy, Mogridge, Ravi S, Kane   +9 more
openaire   +2 more sources