Results 31 to 40 of about 4,795 (186)
Telomere Protection by TPP1 Is Mediated by POT1a and POT1b [PDF]
Molecular and Cellular Biology, 2010 Mammalian telomeres are protected by the shelterin complex, which contains single-stranded telomeric DNA binding proteins (POT1a and POT1b in rodents, POT1 in other mammals). Mouse POT1a prevents the activation of the ATR kinase and contributes to the repression of the nonhomologous end-joining pathway (NHEJ) at newly replicated telomeres.
Tatsuya, Kibe +3 more
openaire +2 more sources
Telomere Protection by TPP1/POT1 Requires Tethering to TIN2 [PDF]
Molecular Cell, 2011 To prevent ATR activation, telomeres deploy the single-stranded DNA binding activity of TPP1/POT1a. POT1a blocks the binding of RPA to telomeres, suggesting that ATR is repressed through RPA exclusion. However, comparison of the DNA binding affinities and abundance of TPP1/POT1a and RPA indicates that TPP1/POT1a by itself is unlikely to exclude RPA. We
Takai, Kaori K. +4 more
openaire +4 more sources
TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres [PDF]
Molecular Cell, 2016 The regulation of 5' end resection at DSBs and telomeres prevents genome instability. DSB resection is positively and negatively regulated by ATM signaling through CtIP/MRN and 53BP1-bound Rif1, respectively. Similarly, telomeres lacking TRF2 undergo ATM-controlled CtIP-dependent hyper-resection when the repression by 53BP1/Rif1 is alleviated. However,
Kibe, Tatsuya +2 more
openaire +4 more sources
Inducible transgenic expression of tripeptidyl peptidase 1 in a mouse model of late-infantile neuronal ceroid lipofuscinosis. [PDF]
PLoS ONE, 2018 Late-infantile neuronal ceroid lipofuscinosis is a fatal neurodegenerative disease of children caused by mutations resulting in loss of activity of the lysosomal protease, tripeptidyl peptidase 1 (TPP1).
Yuliya Nemtsova +4 more
doaj +1 more source
Confirmation of the composition of the POT1-TPP1-TIN2(1–354) complex by immunoblotting analysis.
, 2022 (A) POT1-TPP1-TIN2(1–354) complex integrity analysis by WEMSA. 32P-labelled telo64 telomeric DNA ligand at a concentration of 20 nM (upper panel) or 10 nM (middle and lower panels) was incubated alone (−) or with increasing quantities (10 pM to 100 nM ...
Simon Lattmann (540093) +4 more
core +1 more source
Telomerase and Chromosome End Protection In Vivo: the TPP1 Connection [PDF]
Developmental Cell, 2010 In an article published in this issue of Developmental Cell, Maria Blasco's group shows that the telomere end-binding protein TPP1 is involved in both end protection and telomerase regulation in vivo. Importantly, they highlight the relevance of telomerase activity in highly proliferative tissues and in reprogramming of cells to induced pluripotency ...
Gilson, Eric, Teixeira, M. Teresa
openaire +3 more sources
Cells, 2020 CLN2 Batten disease (BD) is one of a broad class of lysosomal storage disorders that is characterized by the deficiency of lysosomal enzyme, TPP1, resulting in a build-up of toxic intracellular storage material in all organs and subsequent damage.
Matthew J. Haney +3 more
doaj +1 more source
Interaction hub critical for telomerase recruitment and primer-template handling for catalysis
Life Science Alliance, 2023 By deleting, mutating, and adding back parts of the telomerase enzyme that extends chromosome ends, we uncover features important for its proper functioning in the cell.
Shilpa Padmanaban +2 more
doaj +1 more source
, 2022 Straight lines represent the 280 nm absorbance and dotted lines represent the 260 nm absorbance. The blue traces represent the elution profile of an injection of 600 pmol (1×) POT1-TPP1-TIN2(1–354).
Simon Lattmann (540093) +4 more
core +1 more source
Cells, 2023 Over the recent decades, the use of extracellular vesicles (EVs) has attracted considerable attention. Herein, we report the development of a novel EV-based drug delivery system for the transport of the lysosomal enzyme tripeptidyl peptidase-1 (TPP1) to ...
Nazira El-Hage +8 more
doaj +1 more source