Results 31 to 40 of about 4,429 (159)
, 2022 The various 32P-labelled telomeric DNAs were incubated alone (−) or with increasing quantities (10 pM to 100 nM) of POT1-TPP1-TIN2(1–354) protein complex. The binding reaction products were separated by native PAGE.
Simon Lattmann (540093) +4 more
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Mitoribosomal Deregulation Drives Senescence via TPP1-Mediated Telomere Deprotection
Cells, 2022 While mitochondrial bioenergetic deregulation has long been implicated in cellular senescence, its mechanistic involvement remains unclear. By leveraging diverse mitochondria-related gene expression profiles derived from two different cellular senescence models of human diploid fibroblasts, we found that the expression of mitoribosomal proteins (MRPs ...
Seongki Min +6 more
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Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germline [PDF]
Life Science Alliance, 2021 ABSTRACT Telomerase extends chromosome ends in somatic and germline stem cells to ensure continued proliferation. Mutations in genes critical for telomerase function result in telomeropathies such as dyskeratosis congenita (DC), frequently resulting in spontaneous bone marrow failure.
Jacqueline V Graniel +19 more
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Phosphorylation of TPP1 regulates cell cycle-dependent telomerase recruitment [PDF]
Proceedings of the National Academy of Sciences, 2013 Telomere maintenance is essential for organisms with linear chromosomes and is carried out by telomerase during cell cycle. The precise mechanism by which cell cycle controls telomeric access of telomerase and telomere elongation in mammals remains largely unknown.
Yi, Zhang +7 more
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Differential expression of TPP1 in amyotrophic lateral sclerosis.
, 2022 Neurodegenerative diseases that affect the motor neurons, including amyotrophic lateral sclerosis (ALS), have little treatment options and are generally rapidly fatal (1, 2).
Shahan Mamoor
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Coordinated Interactions of Multiple POT1-TPP1 Proteins with Telomere DNA [PDF]
Journal of Biological Chemistry, 2013 Telomeres are macromolecular nucleoprotein complexes that protect the ends of eukaryotic chromosomes from degradation, end-to-end fusion events, and from engaging the DNA damage response. However, the assembly of this essential DNA-protein complex is poorly understood.
Mark, Corriveau +4 more
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Modern genome editing meets telomeres: the many functions of TPP1 [PDF]
Genes & Development, 2014 The telomeric complex has been analyzed in detail for its role in regulating telomere protection and telomere length. Now, modern genome-editing techniques in human embryonic stem cells reveal TPP1 as the essential recruitment factor for telomerase, with additional functions in telomerase activation and definition of telomere length homeostasis.
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Biodistribution of TPP1 preparations.
, 2012 Tpp1(−/−) mice were administered indicated amounts of enzyme via tail vein injection and tissues collected after 24 hours. TPP1 specific activity (activity/mg protein) was normalized to that of wild-type controls.
Istvan Sohar (152558) +4 more
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Thermostability of TPP1 preparations.
, 2012 Left Panels: Proenzyme was incubated at pH 7.2 at different temperatures and then frozen at the indicated times. Preparations were subsequently autoactivated at pH 3.5 for 1.5 h at 37°C prior to measuring enzyme activity.
Istvan Sohar (152558) +4 more
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Plasma circulatory half-life of TPP1 preparations.
, 2012 Tpp1(−/−) mice were administered 0.12 mg (Top) or 2 mg (Bottom) of unmodified or modified TPP1 via tail vein injection and plasma samples collected via cheek puncture at indicated times.
Istvan Sohar (152558) +4 more
core +1 more source