Results 21 to 30 of about 4,429 (159)

The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection [PDF]

Nature Structural & Molecular Biology, 2011
The mammalian shelterin component TPP1 has essential roles in telomere maintenance and, together with POT1, is required for the repression of DNA damage signaling at telomeres. Here we show that in Mus musculus, the E3 ubiquitin ligase Rnf8 localizes to uncapped telomeres and promotes the accumulation of DNA damage proteins 53Bp1 and γ-H2ax.
Zheng, H, Rai, R, Li, JM, Lok, GTM, Deng, Y, Huen, MSY, Chen, J, Jin, J, Chang, S   +8 more
openaire   +5 more sources

TPP1 is associated with risk of advanced precursors and cervical cancer survival

PLOS ONE
It is unclear how telomere-binding protein TPP1 interacts with human telomerase reverse transcriptase (hTERT) and influences cervical cancer development and progression. This study included all eligible 156 cervical cancers diagnosed during 2003–2008 and followed up through 2014, 102 cervical intraepithelial neoplasia (CIN) patients, and 16 ...
Qiao-Li Wang, Caifeng Gong, Xiang-Yu Meng, Min Fu, Hui Yang, Fuxiang Zhou, Qiuji Wu, Yunfeng Zhou   +7 more
openaire   +6 more sources

Zebrafish as a Model System to Study the Physiological Function of Telomeric Protein TPP1

PLoS ONE, 2011
Telomeres are specialized chromatin structures at the end of chromosomes. Telomere dysfunction can lead to chromosomal abnormalities, DNA damage responses, and even cancer. In mammalian cells, a six-protein complex (telosome/shelterin) is assembled on the telomeres through the interactions between various domain structures of the six telomere proteins (
Yiying Xie, Dong Yang, Quanyuan He, Zhou Songyang   +3 more
openaire   +4 more sources

Telomere Protection by TPP1 Is Mediated by POT1a and POT1b [PDF]

Molecular and Cellular Biology, 2010
Mammalian telomeres are protected by the shelterin complex, which contains single-stranded telomeric DNA binding proteins (POT1a and POT1b in rodents, POT1 in other mammals). Mouse POT1a prevents the activation of the ATR kinase and contributes to the repression of the nonhomologous end-joining pathway (NHEJ) at newly replicated telomeres.
Tatsuya, Kibe, Gail A, Osawa, Catherine E, Keegan, Titia, de Lange   +3 more
openaire   +2 more sources

Telomere Protection by TPP1/POT1 Requires Tethering to TIN2 [PDF]

Molecular Cell, 2011
To prevent ATR activation, telomeres deploy the single-stranded DNA binding activity of TPP1/POT1a. POT1a blocks the binding of RPA to telomeres, suggesting that ATR is repressed through RPA exclusion. However, comparison of the DNA binding affinities and abundance of TPP1/POT1a and RPA indicates that TPP1/POT1a by itself is unlikely to exclude RPA. We
Takai, Kaori K., Kibe, Tatsuya, Donigian, Jill R., Frescas, David, de Lange, Titia   +4 more
openaire   +4 more sources

TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres [PDF]

Molecular Cell, 2016
The regulation of 5' end resection at DSBs and telomeres prevents genome instability. DSB resection is positively and negatively regulated by ATM signaling through CtIP/MRN and 53BP1-bound Rif1, respectively. Similarly, telomeres lacking TRF2 undergo ATM-controlled CtIP-dependent hyper-resection when the repression by 53BP1/Rif1 is alleviated. However,
Kibe, Tatsuya, Zimmermann, Michal, de Lange, Titia   +2 more
openaire   +4 more sources

Confirmation of the composition of the POT1-TPP1-TIN2(1–354) complex by immunoblotting analysis.

, 2022
(A) POT1-TPP1-TIN2(1–354) complex integrity analysis by WEMSA. 32P-labelled telo64 telomeric DNA ligand at a concentration of 20 nM (upper panel) or 10 nM (middle and lower panels) was incubated alone (−) or with increasing quantities (10 pM to 100 nM ...
Simon Lattmann (540093), Daniela Rhodes (11349461), Emmanuel W. Smith (1576297), Zhehui Barry Liu (12101806), Bilal Ahsan (12101809)   +4 more
core   +1 more source

Analytical Size Exclusion Chromatography of the POT1-TPP1-TIN2(1–354) complex bound to a single-stranded telomeric substrate GGTTAGGGTTAG (sstelo64).

, 2022
Straight lines represent the 280 nm absorbance and dotted lines represent the 260 nm absorbance. The blue traces represent the elution profile of an injection of 600 pmol (1×) POT1-TPP1-TIN2(1–354).
Simon Lattmann (540093), Daniela Rhodes (11349461), Emmanuel W. Smith (1576297), Zhehui Barry Liu (12101806), Bilal Ahsan (12101809)   +4 more
core   +1 more source

Telomerase and Chromosome End Protection In Vivo: the TPP1 Connection [PDF]

Developmental Cell, 2010
In an article published in this issue of Developmental Cell, Maria Blasco's group shows that the telomere end-binding protein TPP1 is involved in both end protection and telomerase regulation in vivo. Importantly, they highlight the relevance of telomerase activity in highly proliferative tissues and in reprogramming of cells to induced pluripotency ...
Gilson, Eric, Teixeira, M. Teresa
openaire   +3 more sources

A new mutant at the end: TPP1, telomeres, and BMF [PDF]

Blood, 2014
In this issue of Blood, Guo et al identify a mutation in ACD, which encodes the protein TPP1, as the underlying cause of short telomeres and bone marrow failure (BMF) in a family.1 They show that the mutation results in a defect in the recruitment of telomerase to telomeres.
openaire   +2 more sources