Results 11 to 20 of about 4,429 (159)
TPP1 mutagenesis screens unravel shelterin interfaces and functions in hematopoiesis [PDF]
JCI Insight, 2021 Telomerase catalyzes chromosome end replication in stem cells and other long-lived cells. Mutations in telomerase or telomere-related genes result in diseases known as telomeropathies. Telomerase is recruited to chromosome ends by the ACD/TPP1 protein (TPP1 hereafter), a component of the shelterin complex that protects chromosome ends from unwanted end
Sherilyn Grill +10 more
openaire +4 more sources
POT1-TPP1 Regulates Telomeric Overhang Structural Dynamics [PDF]
Structure, 2012 Human telomeres possess a single-stranded DNA (ssDNA) overhang of TTAGGG repeats, which can self-fold into a G-quadruplex structure. POT1 binds specifically to the telomeric overhang and partners with TPP1 to regulate telomere lengthening and capping, although the mechanism remains elusive.
Hwang, Helen +3 more
openaire +3 more sources
Functional interaction between telomere protein TPP1 and telomerase [PDF]
Genes & Development, 2010 Human chromosome end-capping and telomerase regulation require POT1 (Protection of Telomeres 1) and TPP1 proteins, which bind to the 3′ ssDNA extension of human telomeres. POT1–TPP1 binding to telomeric DNA activates telomerase repeat addition processivity.
Zaug, Arthur J +3 more
openaire +4 more sources
TIN2 Functions with TPP1/POT1 To Stimulate Telomerase Processivity [PDF]
Molecular and Cellular Biology, 2019 TIN2 is an important regulator of telomere length, and mutations in TINF2, the gene encoding TIN2, cause short-telomere syndromes. While the genetics underscore the importance of TIN2, the mechanism through which TIN2 regulates telomere length remains unclear. Here, we tested the effects of human TIN2 on telomerase activity. We identified a new isoform
Alexandra M. Pike +3 more
core +10 more sources
TIN2-Tethered TPP1 Recruits Human Telomerase to Telomeres In Vivo [PDF]
Molecular and Cellular Biology, 2010 Recruitment to telomeres is a pivotal step in the function and regulation of human telomerase; however, the molecular basis for recruitment is not known. Here, we have directly investigated the process of telomerase recruitment via fluorescence in situ hybridization (FISH) and chromatin immunoprecipitation (ChIP).
Abreu E +7 more
openaire +5 more sources
The Shelterin Component TPP1 Is a Binding Partner and Substrate for the Deubiquitinating Enzyme USP7 [PDF]
Journal of Biological Chemistry, 2014 The telomeric shelterin component TPP1 has critical functions in telomeric protein complex assembly and telomerase recruitment and regulation. Here we identify USP7 as a novel interacting protein of the oligonucleotide/oligosaccharide-binding fold of TPP1, which was previously known to recruit telomerase to telomeres.
Zemp Ivo, Lingner Joachim
openaire +4 more sources
Structure of active human telomerase with telomere shelterin protein TPP1 [PDF]
Nature, 2022 Human telomerase is a RNA-protein complex that extends the 3' end of linear chromosomes by synthesizing multiple copies of the telomeric repeat TTAGGG1. Its activity is a determinant of cancer progression, stem cell renewal and cellular aging2-5. Telomerase is recruited to telomeres and activated for telomere repeat synthesis by the telomere shelterin ...
Baocheng Liu +5 more
openaire +4 more sources
Structural and functional analysis of the human POT1-TPP1 telomeric complex [PDF]
Nature Communications, 2017 Abstract POT1 and TPP1 are part of the shelterin complex and are essential for telomere length regulation and maintenance. Naturally occurring mutations of the telomeric POT1–TPP1 complex are implicated in familial glioma, melanoma and chronic lymphocytic leukaemia. Here we report the atomic structure of the interacting portion of the
Rice, C +8 more
openaire +7 more sources
A Rare Variant P507L in TPP1 Interrupts TPP1–TIN2 Interaction, Influences Telomere Length, and Confers Colorectal Cancer Risk in Chinese Population [PDF]
Cancer Epidemiology, Biomarkers & Prevention, 2018 Abstract Background: Telomere dysfunction triggers cellular senescence and constitutes a driving force for cancer initiation. Genetic variants in genes involved in telomere maintenance may contribute to colorectal cancer susceptibility.
Jiaoyuan, Li +16 more
openaire +3 more sources
Dynamic peptides of human TPP1 fulfill diverse functions in telomere maintenance [PDF]
Nucleic Acids Research, 2016 Telomeres are specialized nucleoprotein complexes that comprise the ends of linear chromosomes. Human telomeres end in a short, single-stranded DNA (ssDNA) overhang that is recognized and bound by two telomere proteins, POT1 and TPP1. Whereas POT1 binds directly to telomere ssDNA, its interaction with TPP1 is essential for localization of POT1 to the ...
Rajavel, Malligarjunan +6 more
openaire +5 more sources