Intranuclear topological distribution of HIV-1 trans-activators [PDF]
FEBS Letters, 1992 Subcellular localization of human immunodeficiency virus type 1 (HIV-1) Tat and Rev was examined using a confocal laser scanning microscope (CLSM). In transfected COS-7 cells, Tat resided exclusively in the perinocleolar region, while Rev infiltrated ...
Fujita, Setsuya +4 more
core +7 more sources
Characterization of the transcriptional trans activator of human foamy retrovirus [PDF]
Journal of Virology, 1991 The human foamy viruses, or spumaviruses, a distinct subfamily of complex human retroviruses, remain poorly understood both in terms of their pathogenic potential and in terms of the regulatory mechanisms that govern their replication. Here, we demonstrate that the human spumaretrovirus shares with other complex human retroviruses the property of ...
Andreas Keller +5 more
semanticscholar +5 more sources
Posttranscriptional trans-activation in cauliflower mosaic virus
Cell, 1989 The ability of plant cells to translate dicistronic mRNAs that mimic a segment of the polycistronic 35S RNA from cauliflower mosaic virus has been tested. The chloramphenicol acetyltransferase and beta-glucuronidase open reading frames (ORFs) were fused in-frame to the second viral cistron (ORF I).
Jean‐Marc Bonneville +3 more
openalex +4 more sources
Bioassay for trans-activation using purified human immunodeficiency virus tat-encoded protein: trans-activation requires mRNA synthesis. [PDF]
Proceedings of the National Academy of Sciences, 1989 Expression of the human immunodeficiency virus tat-encoded protein (Tat) is required for virus replication. A genetic approach was used to facilitate the purification of biologically active Tat. A recombinant Tat protein containing a stretch of six histidine residues and a protease cleavage site was engineered and purified to greater than 95 ...
Reiner Gentz, C H Chen, C A Rosen
openalex +4 more sources
trans-Activation of parvovirus P38 promoter by the 76K noncapsid protein [PDF]
Journal of Virology, 1985 The autonomously replicating parvoviruses contain a 5-kilobase linear single-stranded DNA genome that produces two noncapsid proteins, N1 and N2, and two overlapping capsid proteins, VP1 and VP2. To characterize the regulation of viral transcription, we began with a study of the promoter for the coat proteins (P38) at map unit 38. Various constructions
S L Rhode
openalex +5 more sources
Specific multivalent molecules boost CRISPR-mediated transcriptional activation [PDF]
Nature CommunicationsCRISPR/Cas-based transcriptional activators can be enhanced by intrinsically disordered regions (IDRs). However, the underlying mechanisms are still debatable.
Rui Chen +15 more
doaj +2 more sources
Cis- and Trans-Activation of Hormone Receptors: the LH Receptor [PDF]
Molecular Endocrinology, 2002 AbstractG protein-coupled receptors (GPCRs) accommodate a wide spectrum of activators from ions to glycoprotein hormones. The mechanism of activation for this large and clinically important family of receptors is poorly understood. Although initially thought to function as monomers, there is a growing body of evidence that GPCR dimers form, and in some
P. Michael Conn +4 more
openaire +4 more sources
Stimulation of baculovirus transcriptome expression in mammalian cells by baculoviral transcriptional activators [PDF]
Journal of General Virology, 2014 Autographa californica multiple n ucleopolyhedro virus (AcMNPV), the type species of the family Baculoviridae, is an insect-specific virus that can enter a variety of mammalian cells.
Chao, Y.C. +3 more
core +2 more sources
Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics [PDF]
Communications BiologyCas12 and Cas13 are extensively utilized in molecular diagnostics for their trans-cleavage activities, yet their activation characteristics remain partially understood. Here, we conduct an in-depth investigation of Cas12a, Cas12f1, and Cas13a, uncovering
Qing Wu +7 more
doaj +2 more sources
Coactivators p300 and PCAF physically and functionally interact with the foamy viral trans-activator [PDF]
BMC Molecular Biology, 2004 BACKGROUND: Foamy virus Bel1/Tas trans-activators act as key regulators of gene expression and directly bind to Bel1 response elements (BRE) in both the internal and the 5'LTR promoters leading to strong transcriptional trans-activation.
Bannert, Helmut +4 more
core +4 more sources