Results 211 to 220 of about 736,695 (298)

RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System

Advanced Science, EarlyView.
Condensates at synapses organize synaptic vesicles (SVs) and are crucial for efficient neurotransmitter release, yet how RNA contributes to this mesoscale architecture remains poorly understood. Here we uncover that RNA modulates synapsin–driven condensates.
Branislava Rankovic, Zachary M. Geisterfer, Akshita Chhabra, Vladimir M. Jovanovic, Ian Seim, Christian Hoffmann, Antonela Condric, Gerard Aguilar Pérez, Rinse de Boer, Lars T. Hofstede, Jingao Sun, Kiara Freitag, Katja Nowick, Marina Jendrach, Frank L. Heppner, Vladimir Despic, Michaela Müller‐McNicoll, Stephan Sigrist, Geert van den Bogaart, Sofiia Reshetniak, Amy S. Gladfelter, Dragomir Milovanovic   +21 more
wiley   +1 more source

Use of translational fusions to express functional Klebsiella oxytoca dinitrogenase reductase in plant mitochondria. [PDF]

Plant Mol Biol
Okada S, Wang X, Gregg CM, Allen RS, Rhodes T, Gillespie V, Venables I, Mathew A, Bilyj JK, Byrne K, DeFeyter RC, Rapson TD, Wood CC.   +12 more
europepmc   +1 more source

Structural energetics of the adenine tract from an intrinsic transcription terminator. [PDF]

J Mol Biol, 2010
Huang Y, Weng X, Russu IM.
europepmc   +1 more source

A Multidimensional Engineering Strategy Reprograms Microglia via Targeted and Sustained‐Release Extracellular Vesicles for Spinal Cord Injury Repair

Advanced Science, EarlyView.
A multifunctional EV‐based nanoplatform (Ang‐TEVs@Gel) was engineered via preconditioning, surface targeting, and ROS‐responsive hydrogel encapsulation to reprogram microglia. This system restored autophagy via miR‐664a‐3p/PIK3CA axis, cleared myelin debris, resolved neuroinflammation, and promoted axon remyelination, ultimately achieving robust motor ...
Wu Xiong, Minhao Liu, Mingming Zheng, Jizhou Jia, Ziyan Zhu, Guang Kong, Jie Liu, Juan Wang, Wenbo Li, Qingyuan Wang, Qian Zhu, Peiran Chan, Tao Qin, Jiayang Wu, Yongjie Zhang, Chunming Tang, Cong Li, Jin Fan   +17 more
wiley   +1 more source

RNA-Guided Engineering of the Chloroplast Genome Enabled by Plastid-Expressed Guide RNAs. [PDF]

Plant Biotechnol J
Mirzaee M, Best C, Wachowski EV, Maliga P.   +3 more
europepmc   +1 more source

BOULE is Essential for the Dynamic Disassembly of Heat Shock Granules in Male Germ Cells

Advanced Science, EarlyView.
BOULE orchestrates stress granule disassembly in germ cells via a two‐pronged mechanism: it promotes G3BP1 ubiquitination by upregulating TRIM27, generating a signal for VCP/FAF2 recruitment, and it maintains G3BP1 and FAF2 protein levels. BOULE deficiency disrupts disassembly complex formation, leading to impaired heat shock granule clearance ...
Xin Li, Nana Zhang, Zexin Bian, Baobao Geng, Liyun Cao, Peipei Liu, Zengming Li, Yan Zhao, Jun Tan   +8 more
wiley   +1 more source

Leaky recombinant expression reveals design constraints of bicistronic synthetic operons in Escherichia coli. [PDF]

Sci Rep
Gutmann S, Tauer C, Wagenknecht M, Striedner G, Grabherr R, Cserjan-Puschmann M.   +5 more
europepmc   +1 more source

Deep Learning‐Driven Discovery and Engineering of an Efficient PETase for Depolymerization and Detoxification of PET Microplastics Under Physiological Conditions

Advanced Science, EarlyView.
A deep learning–driven pipeline mining 246 million protein sequences uncovers AhPETase, an evolutionarily distinct PET hydrolase. Engineered variant AhPETaseM1 degrades post‐consumer PET microplastics under physiological conditions and reverses microplasticinduced cytotoxicity in human lung and colon cells, establishing enzymatic microplastic ...
Yuxuan Wang, Shijie He, Yuheng Chang, Sheng Mao, Jianan Canal Li, Binbin Chen, Hongxun Gao, Mingchun Xu, Chenxu Liu, Yajie Wang   +9 more
wiley   +1 more source

State-dependent promoter switching mediates integrase gene transcription in the integrative and conjugative element ICEKp1 of Klebsiella pneumoniae. [PDF]

Nucleic Acids Res
Liu G, Huang Z, Guan J, Tai C, Shi L, Chao Y, Wang R, Wang K, Ou HY.   +8 more
europepmc   +1 more source

The Human RNA Polymerase I Transcription Terminator Complex Acts as a Replication Fork Barrier That Coordinates the Progress of Replication with rRNA Transcription Activity. [PDF]

Mol Cell Biol, 2015
Akamatsu Y, Kobayashi T.
europepmc   +1 more source