Widespread transcriptional pausing and elongation control at enhancers
In this study, Henriques et al. demonstrate that transcription is a nearly universal feature of enhancers in Drosophila and mammalian cells and that nascent RNA sequencing strategies are optimal for identification of both enhancers and superenhancers ...
Telmo Henriques +8 more
semanticscholar +1 more source
Transcription elongation factor hSPT5 stimulates mRNA capping [PDF]
RNA polymerase II nascent transcripts are capped during pausing before elongation. Here we report that hSPT5, the human homolog of yeast elongation factor SPT5, interacts directly with the capping enzyme. hSPT5 stimulated capping enzyme guanylylation and mRNA capping by severalfold.
Y, Wen, A J, Shatkin
openaire +2 more sources
Tomato EF-Tsmt, a functional mitochondrial translation elongation factor from higher plants [PDF]
Ethylene-induced ripening in tomato (Lycopersicon esculentum) resulted in the accumulation of a transcript designated LeEF-Tsmt that encodes a protein with significant homology to bacterial Ts translational elongation factor (EF-Ts).
Jauneau, Alain +13 more
core +1 more source
System Analysis of MIRNAs in Maize Internode Elongation
MicroRNAs (miRNAs), the post-transcriptional gene regulators, are known to play an important role in plant development. The identification of differentially expressed miRNAs could better help us understand the post-transcriptional regulation that occurs ...
Chuanxi Peng +7 more
doaj +1 more source
Reading of the non‐template DNA by transcription elongation factors [PDF]
SummaryUnlike transcription initiation and termination, which have easily discernable signals, such as promoters and terminators, elongation is regulated through a dynamic network involving RNA/DNA pause signals and states‐rather than sequence‐specific protein interactions. A report by Nedialkov et al.
Vladimir Svetlov, Evgeny Nudler
openaire +2 more sources
RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP [PDF]
Through controlling the nuclear level of active positive transcription elongation factor b (P-TEFb), the 7SK small nuclear RNA (snRNA) functions as a key regulator of RNA polymerase II transcription.
Kiss, T. +4 more
core +1 more source
Affinity chromatography on columns containing the immobilized monomeric transcriptional elongation factor TFIIS or the essential large subunit, Elongin A, of the trimeric elongation factor, Elongin, was used to purify a human RNA polymerase II holoenzyme
Guo-Hua Pan, T. Aso, J. Greenblatt
semanticscholar +1 more source
FACT, a Factor that Facilitates Transcript Elongation through Nucleosomes [PDF]
The requirements for transcriptional activation by RNA polymerase II were examined using chromatin templates assembled in vitro and a transcription system composed of the human general transcription factors and RNA polymerase II. Activator-induced, energy-dependent chromatin remodeling promoted efficient preinitiation complex formation and ...
Orphanides, George +4 more
openaire +2 more sources
A conserved phosphorylation site regulates the transcriptional function of ETHYLENE-INSENSITIVE3-like1 in tomato [PDF]
ETHYLENE-INSENSITIVE3/ETHYLENE-INSENSITIVE3-like (EIN3/EIL) transcription factors are important downstream components of the ethylene transduction pathway known to regulate the transcription of early ethylene-responsive genes in plants.
Z. Li +11 more
core +1 more source
NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble
Universally conserved factors from NusG family bind at the upstream fork junction of transcription elongation complexes and modulate RNA synthesis in response to translation, processing, and folding of the nascent RNA.
Matti Turtola, Georgiy A Belogurov
doaj +1 more source

