EloA promotes HEL polyploidization upon PMA stimulation through enhanced ERK1/2 activity
Platelets, 2022 Megakaryocytes (MKs) are the unique non-pathological cells that undergo polyploidization in mammals. The polyploid formation is critical for understanding the MK biology, and transcriptional regulation is involved in the differentiation and maturation of
Lanyue Hu +21 more
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Solid phase chemistry to covalently and reversibly capture thiolated RNA. [PDF]
, 2018 Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s4U) metabolic labeling and solid phase chemistry.
Canzio, Daniele +3 more
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ISWI and CHD chromatin remodelers bind promoters but act in gene bodies. [PDF]
PLoS Genetics, 2013 ATP-dependent nucleosome remodelers influence genetic processes by altering nucleosome occupancy, positioning, and composition. In vitro, Saccharomyces cerevisiae ISWI and CHD remodelers require ∼30-85 bp of extranucleosomal DNA to reposition nucleosomes,
Gabriel E Zentner +2 more
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Nature Communications, 2023 Binding of transcription factors (TFs) promotes the subsequent recruitment of coactivators and preinitiation complexes to initiate eukaryotic transcription, but this time course is usually not visualized.
Chun-Yi Cho, Patrick H. O’Farrell
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Translesion synthesis DNA polymerase η exhibits a specific RNA extension activity and a transcription-associated function [PDF]
, 2017 We thank Andres Aguilera for providing the pCYC-LacZ plasmid for the GLRO experiments, and Szilvia Minorits for technical assistance. This work was also supported by grants from the National Research, Development and Innovation Office: GINOP-2.3.2-15 ...
Balint, Eva +5 more
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Synthetic transcription elongation factors license transcription across repressive chromatin [PDF]
Science, 2017 Chemical control of transcription Friedreich's ataxia, a devastating neurodegenerative disease with no effective therapy, is caused by an expansion of intronic repeats and hence a reduced expression of the FXN gene. Erwin et al.
Graham S. Erwin +21 more
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Towards molecular systems biology of gene transcription and regulation [PDF]
, 2010 Ten years after the determination of the RNA polymerase 11 structure, the basic mechanism of mRNA synthesis during gene transcription is known. In the future, the initiation and regulation of transcription must be studied with a combination of structural
Cramer, Patrick
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Involvement of Transcription Termination Factor 2 in Mitotic Repression of Transcription Elongation [PDF]
Molecular Cell, 2004 All nuclear transcription is interrupted during mitosis. We examined the role of human TTF2, an RNA polymerase (Pol) I and II termination factor, in mitotic repression of transcription elongation. We find that TTF2 levels rise in the cytoplasm in S and G2 and at the onset of mitosis TTF2 translocates into the nucleus.
Jiang, Yan +3 more
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Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency. [PDF]
, 2019 BackgroundHIV-infected cell lines are widely used to study latent HIV infection, which is considered the main barrier to HIV cure. We hypothesized that these cell lines differ from each other and from cells from HIV-infected individuals in the mechanisms
Aran, Dvir +10 more
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Coupling of Transcription and Translation in Archaea: Cues From the Bacterial World
Frontiers in Microbiology, 2021 The lack of a nucleus is the defining cellular feature of bacteria and archaea. Consequently, transcription and translation are occurring in the same compartment, proceed simultaneously and likely in a coupled fashion.
Albert Weixlbaumer +7 more
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