Results 81 to 90 of about 418,457 (205)

Targeted Gene Delivery through the Respiratory System: Rationale for Intratracheal Gene Transfer

Journal of Cardiovascular Development and Disease, 2019
Advances in DNA- and RNA-based technologies have made gene therapy suitable for many lung diseases, especially those that are hereditary. The main objective of gene therapy is to deliver an adequate amount of gene construct to the intended target cell ...
Michael G. Katz, Anthony S. Fargnoli, Sarah M. Gubara, Kenneth Fish, Thomas Weber, Charles R. Bridges, Roger J. Hajjar, Kiyotake Ishikawa   +7 more
doaj   +1 more source

An efficient method for the transduction of primary pediatric glioma neurospheres

MethodsX, 2018
Pediatric high grade glioma (pHGG) and diffuse intrinsic pontine glioma (DIPG) are rare, but rapidly fatal malignancies of the central nervous system (CNS), and the leading cause of cancer-related death in children.
Michaël H. Meel, Dennis S. Metselaar, Piotr Waranecki, Gertjan J.L. Kaspers, Esther Hulleman   +4 more
doaj   +1 more source

Long-term in vitro monitoring of AAV-transduction efficiencies in real-time with Hoechst 33342.

PLoS ONE
Adeno-associated viral transduction allows the introduction of nucleic fragments into cells and is widely used to modulate gene expressions in vitro and in vivo.
Xiaonan Hu, Roland Meister, Jan Tode, Carsten Framme, Heiko Fuchs   +4 more
doaj   +1 more source

Genome hypermobility by lateral transduction [PDF]

, 2018
Genetic transduction is a major evolutionary force that underlies bacterial adaptation. Here we report that the temperate bacteriophages of Staphylococcus aureus engage in a distinct form of transduction we term lateral transduction.
Bacigalupe, Rodrigo, Fitzgerald, J. Ross, Fillol-Salom, Alfred, Quiles-Puchalt, Nuria, Chee, Melissa Su Juan, Chiang, Yin Ning, Penadés, José R., Chen, John, Penadés, José R, Fitzgerald, Jonathan; id_orcid, Fillol-Salom, Alfredo, Fillo-Salom, Alfred   +11 more
core   +1 more source

Double-strand break repair pathways differentially affect processing and transduction by dual AAV vectors

Nature Communications
Recombinant adeno-associated viral vectors (rAAV) are a powerful tool for gene delivery but have a limited DNA carrying capacity. Efforts to expand this genetic payload have focused on engineering the vector components, such as dual trans-splicing ...
Anna C. Maurer, Brian Benyamini, Oscar N. Whitney, Vinson B. Fan, Claudia Cattoglio, Djem U. Kissiov, Gina M. Dailey, Xavier Darzacq, Matthew D. Weitzman, Robert Tjian   +9 more
doaj   +1 more source

A conserved phosphorylation site regulates the transcriptional function of ETHYLENE-INSENSITIVE3-like1 in tomato [PDF]

, 2011
ETHYLENE-INSENSITIVE3/ETHYLENE-INSENSITIVE3-like (EIN3/EIL) transcription factors are important downstream components of the ethylene transduction pathway known to regulate the transcription of early ethylene-responsive genes in plants.
Z. Li, J. Li, Li, Zhengguo, M. Bouzayen, Tang, Ling, M. Zouine, L. Tang, Zouine, Mohamed, Y. Yang, Yang, Yingwu, Li, Ji, Bouzayen, Mondher   +11 more
core   +1 more source

Insights for producing and future use of induced pluripotent stem cells from the small red brocket deer (Mazama jucunda)

Theriogenology Wild
The small red brocket (Mazama jucunda), a deer endemic to the Atlantic Forest biome, is considered vulnerable by the IUCN, needing reproductive biotechnologies to preserve its genetic material.
Luana Scarpelini Rosa, Laís Vicari de Figueiredo Pessôa, Nayanne Sant Clair Cardoso da Silva, Jenyffer Mairely Rosero, Kaiana Recchia, José Maurício Barbanti Duarte, Fabiana Fernandes Bressan   +6 more
doaj   +1 more source

Transgenic analysis of thymocyte signal transduction

, 2002
This review examines the value of transgenic studies in mice for the genetic dissection of signal-transduction pathways relevant to thymus development.
Doreen A. Cantrell, Cantrell, Doreen A.
core   +1 more source

Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatant [PDF]

, 2014
We are developing retroviral-mediated gene transfer to human fibroblast-like synovial cells (FLS) as one approach to characterizing genetic pathways involved in synoviocyte pathophysiology.
Friedman, Michael S, Bian, Huimin, McDonagh, Kevin T, Crofford, Leslie J, Yang, Jianmin, Roessler, Blake   +5 more
core   +1 more source

Investigation into the Roles of AAV Genetic Elements During Transduction

Unpublished data under review titled "Analyses of circularized episomal rAAV genomes reveal high rates of truncated and deleted ITRs and transgene cassettes"The first inverted terminal repeat (ITR) elements were sequenced over 40 years ago and are the ...
Namkung, Suk
core   +1 more source