Results 11 to 20 of about 568,858 (297)
Transfection by Electroporation [PDF]
AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
Richard Heller, Huntington Potter
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Transfection of Platyhelminthes [PDF]
Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans.
Juan Pedro Laclette+3 more
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Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent [PDF]
Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection efficiency is a barrier to wider realisation of the potential this model system has to offer.
Sara E. Bodbin+2 more
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Transfection in Micromonospora spp [PDF]
The introduction of bacteriophage DNA into Micromonospora protoplasts, resulting in the production of infective viral progeny, is reported. Transfection was affected by several factors. We observed that it reached a maximum when protoplasts from young mycelium (15 h old) were used.
Caso Machicado, José Luis+2 more
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Tetraethylenepentamine-Coated β Cyclodextrin Nanoparticles for Dual DNA and siRNA Delivery
Nucleic acid reagents, including plasmid-encoded genes and small interfering RNA (siRNA), are promising tools for validating gene function and for the development of therapeutic agents.
Chi-Hsien Liu+5 more
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Tiger puffer fish (Takifugu rubripes) has become the main fish species cultured in China since the last century because of its high economic value. Male and female tiger puffer fish need 2 and 3 years each to reach sexual maturity, which limits the ...
Leilei Tan+8 more
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Annexin A7 and Its Related Protein Suppressor of Death Domains Regulates Migration and Proliferation of Hca-P Cells [PDF]
Objective: This study was to investigate whether annexin A7 (AnnexinA7, ANXA7) and its co-related protein tumor celldeath domain silencer [suppressor of death domains (SODD)] regulates the migratory phenotype of liver cancer cells.Materials and Methods ...
Shaoqing Wang+5 more
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Microglia, the resident immune cells of the brain, have multiple functions in physiological and pathological conditions, including Alzheimer’s disease (AD).
Alejandro Carrillo-Jimenez+8 more
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Haunted Bodies: Cell Switching, Getting Lost and Adaptive Geographies [PDF]
This article proposes the ideas of stochastic resonance and noise as devices with which to think of the body or self as plural and porous. Boundaries and surfaces are proposed as indefinite; cell switching and narratives of the self are discussed in ...
Jane Grant, Joanne “Bob” Whalley
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Background: In our previous study, HSA-PEI demonstrated high pDNA transfection efficiency and low cytotoxicity. Materials and Methods: In this study, the relationship between albumin receptors and the high pDNA transfection efficiency of HSA-PEI was ...
Ssu-Wei Peng+4 more
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