Results 11 to 20 of about 568,858 (297)

Transfection by Electroporation [PDF]

open access: yesCurrent Protocols in Cell Biology, 1992
AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
Richard Heller, Huntington Potter
openaire   +13 more sources

Transfection of Platyhelminthes [PDF]

open access: yesBioMed Research International, 2015
Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans.
Juan Pedro Laclette   +3 more
openaire   +2 more sources

Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent [PDF]

open access: yesMethods and Protocols, 2020
Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection efficiency is a barrier to wider realisation of the potential this model system has to offer.
Sara E. Bodbin   +2 more
openaire   +3 more sources

Transfection in Micromonospora spp [PDF]

open access: yesApplied and Environmental Microbiology, 1987
The introduction of bacteriophage DNA into Micromonospora protoplasts, resulting in the production of infective viral progeny, is reported. Transfection was affected by several factors. We observed that it reached a maximum when protoplasts from young mycelium (15 h old) were used.
Caso Machicado, José Luis   +2 more
openaire   +4 more sources

Tetraethylenepentamine-Coated β Cyclodextrin Nanoparticles for Dual DNA and siRNA Delivery

open access: yesPharmaceutics, 2022
Nucleic acid reagents, including plasmid-encoded genes and small interfering RNA (siRNA), are promising tools for validating gene function and for the development of therapeutic agents.
Chi-Hsien Liu   +5 more
doaj   +1 more source

Establishment and Characterization of a Spermatogonial Stem Cell Line from Tiger Puffer Fish (Takifugu rubripes)

open access: yesAnimals, 2023
Tiger puffer fish (Takifugu rubripes) has become the main fish species cultured in China since the last century because of its high economic value. Male and female tiger puffer fish need 2 and 3 years each to reach sexual maturity, which limits the ...
Leilei Tan   +8 more
doaj   +1 more source

Annexin A7 and Its Related Protein Suppressor of Death Domains Regulates Migration and Proliferation of Hca-P Cells [PDF]

open access: yesCell Journal, 2023
Objective: This study was to investigate whether annexin A7 (AnnexinA7, ANXA7) and its co-related protein tumor celldeath domain silencer [suppressor of death domains (SODD)] regulates the migratory phenotype of liver cancer cells.Materials and Methods ...
Shaoqing Wang   +5 more
doaj   +1 more source

Effective Knockdown of Gene Expression in Primary Microglia With siRNA and Magnetic Nanoparticles Without Cell Death or Inflammation

open access: yesFrontiers in Cellular Neuroscience, 2018
Microglia, the resident immune cells of the brain, have multiple functions in physiological and pathological conditions, including Alzheimer’s disease (AD).
Alejandro Carrillo-Jimenez   +8 more
doaj   +1 more source

Haunted Bodies: Cell Switching, Getting Lost and Adaptive Geographies [PDF]

open access: yesAvant, 2017
This article proposes the ideas of stochastic resonance and noise as devices with which to think of the body or self as plural and porous. Boundaries and surfaces are proposed as indefinite; cell switching and narratives of the self are discussed in ...
Jane Grant, Joanne “Bob” Whalley
doaj   +1 more source

The mechanism of high transfection efficiency of human serum albumin conjugated polyethylenimine in A549 cells

open access: yesJournal of Medical Sciences, 2015
Background: In our previous study, HSA-PEI demonstrated high pDNA transfection efficiency and low cytotoxicity. Materials and Methods: In this study, the relationship between albumin receptors and the high pDNA transfection efficiency of HSA-PEI was ...
Ssu-Wei Peng   +4 more
doaj   +1 more source

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