Results 11 to 20 of about 554,940 (322)
Transfection by Electroporation [PDF]
Current Protocols in Cell Biology, 1992 AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
Richard Heller, Huntington Potter
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Transfection of Platyhelminthes [PDF]
BioMed Research International, 2015 Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans.
Juan Pedro Laclette +3 more
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Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent [PDF]
Methods and Protocols, 2020 Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection efficiency is a barrier to wider realisation of the potential this model system has to offer.
Sara E. Bodbin +2 more
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Frontiers in Molecular Neuroscience, 2018 Overexpression approaches and fluorescence microscopy techniques allow investigating important spatiotemporal aspects of gene regulation as well as quantifying gene function.
Kristin Wendland +10 more
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Transfection in Micromonospora spp [PDF]
Applied and Environmental Microbiology, 1987 The introduction of bacteriophage DNA into Micromonospora protoplasts, resulting in the production of infective viral progeny, is reported. Transfection was affected by several factors. We observed that it reached a maximum when protoplasts from young mycelium (15 h old) were used.
Caso Machicado, José Luis +2 more
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The Thermodynamics of Endosomal Escape and DNA Release from Lipoplexes [PDF]
Phys. Chem. Chem. Phys. 18, 2591 (2016), 2015 Complexes of cationic and neutral lipids and DNA (lipoplexes) are emerging as promising vectors for gene therapy applications. Their appeal stems from their non pathogenic nature and the fact that they self-assemble under conditions of thermal equilibrium. Lipoplex adhesion to the cell plasma membrane initiates a three-stage process termed transfection,
arxiv +1 more source
Exploration of Targeted Anti-tumor Therapy, 2022 Aim: Gene-based immunotherapy against cancer is limited by low gene transfer efficiency. In the literature, interleukin-12 (IL-12) encoding plasmid associated with sonoporation has been shown to enhance antitumoral activity. Moreover, non-viral carriers
Hai Doan Do +10 more
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Predictive Modeling of Non-Viral Gene Transfer [PDF]
Biotechnology and Bioengineering, Volume 105, Issue 4, 805-813,
(2010), 2010 In non-viral gene delivery, the variance of transgenic expression stems from the low number of plasmids successfully transferred. Here, we experimentally determine Lipofectamine- and PEI-mediated exogenous gene expression distributions from single cell time-lapse analysis.
arxiv +1 more source
Plate-based transfection and culturing technique for genetic manipulation of
Malaria Journal, 2012 Genetic manipulation of malaria parasites remains an inefficient, time-consuming and resource-intensive process. Presented here is a set of methods for 96-well plate-based transfection and culture that improve the efficiency of genetic manipulation of ...
Caro Florence +2 more
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Functional differences between protamine preparations for the transfection of mRNA
Drug Delivery, 2020 Protamine is a natural cationic peptide mixture used as a drug for the neutralization of heparin and in formulations of slow-release insulin. In addition, Protamine can be used for the stabilization and delivery of nucleic acids (antisense, small ...
Natalia Teresa Jarzebska +7 more
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