Results 261 to 270 of about 570,469 (297)
Some of the next articles are maybe not open access.
Nature Medicine, 1995
The introduction of novel genes into mosquitoes and malaria parasites is now feasible, raising hopes for an effective attack on malaria.
Donald J. Krogstad, Dawn M. Wesson
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The introduction of novel genes into mosquitoes and malaria parasites is now feasible, raising hopes for an effective attack on malaria.
Donald J. Krogstad, Dawn M. Wesson
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Calcium Phosphate Transfection
Current Protocols in Neuroscience, 1997AbstractThis unit presents two methods of calcium phosphate‐based eukaryotic cell transfection that can be used for both transient and stable transfections. In these protocols, plasmid DNA is introduced to monolayer cell cultures via a precipitate that adheres to the cell surface.
John K. Rose +3 more
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Cold Spring Harbor Protocols, 2018
Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. “Optical transfection”—a physical transfection method—exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.
Priti, Kumar +2 more
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Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. “Optical transfection”—a physical transfection method—exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.
Priti, Kumar +2 more
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Transduction and transfection of difficult‐to‐transfect cells: Systematic attempts for the transfection of protozoa Leishmania [PDF]
Journal of Cellular Biochemistry, 2018 AbstractCell‐penetrating peptides (CPPs) are used to internalize different cargoes, including DNA, into live mammalian and plant cells. Despite many cells being easily transfected with this approach, other cells are rather “difficult” or “hard to transfect,” including protist cells of the genus Leishmania.
Andrea‐Anneliese Keller +6 more
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Current Protocols in Neuroscience, 1997
AbstractThe single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate‐mediated gene transfer, DEAE‐dextran‐mediated gene transfer, electroporation, and liposome‐mediated transfection.
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AbstractThe single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate‐mediated gene transfer, DEAE‐dextran‐mediated gene transfer, electroporation, and liposome‐mediated transfection.
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2003
Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of ...
Paul C, Bridgman +2 more
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Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of ...
Paul C, Bridgman +2 more
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2018
Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
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Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
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2011
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
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Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
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Transfection of Difficult-to-Transfect Primary Mammalian Cells
2011Primary cells are a valuable tool for researchers and are often preferred over transformed or immortalized cell lines since they are biologically more relevant and resemble the in vivo situation much closer. Unfortunately, efficient gene transfer in primary cells is still limited.
Oliver Gresch, Ludger Altrogge
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Liposome‐Mediated Transfection
Current Protocols in Immunology, 1992AbstractUsing liposomes to deliver DNA into different eukaryotic cell types results in higher efficiency and greater reproducibility than other transfection methods. In this unit, the describes a transient expression system while an involves stable transformation and expression of DNA integrated into the genome of the transfected cell.
Linda Buonocore +2 more
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