Small RNA zippers lock miRNA molecules and block miRNA function in mammalian cells. [PDF]
, 2017 MicroRNAs (miRNAs) loss-of-function phenotypes are mainly induced by chemically modified antisense oligonucleotides. Here we develop an alternative inhibitor for miRNAs, termed \u27small RNA zipper\u27.
Chen, Yi-Han +16 more
core +2 more sources
Pharmaceutics, 2022 The generation of reactive oxygen species (ROS) can affect cationic liposome-mediated transfection. In this study, we focused on a specific class of antioxidants, flavonoids, to investigate the transfection efficiency using cationic liposome/plasmid DNA ...
Die Hu +4 more
doaj +1 more source
pH-Sensitive Chitosan–Heparin Nanoparticles for Effective Delivery of Genetic Drugs into Epithelial Cells [PDF]
, 2019 Chitosan has been extensively studied as a genetic drug delivery platform. However, its efficiency is limited by the strength of DNA and RNA binding.
Korzhikov-Vlakh, Viktor +7 more
core +1 more source
Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent [PDF]
Methods and Protocols, 2020 Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection efficiency is a barrier to wider realisation of the potential this model system has to offer.
Sara E. Bodbin +2 more
openaire +3 more sources
Current Issues in Molecular Biology, 2023 Transfection is a contemporary approach for introducing foreign genetic material into target cells. The effective transport of genetic materials into cells is mostly influenced by (a) the characteristics of the genetic material (quantity and quality), (b)
Hanaa Alkharobi
doaj +1 more source
Sustained gene expression in the retina by improved episomal vectors [PDF]
, 2014 Gene and cellular therapies are nowadays part of therapeutic strategies for the treatment of diverse pathologies. The drawbacks associated with gene therapy-low levels of transgene expression, vector loss during mitosis, and gene silencing-need to be ...
Ana V. Oliveira +7 more
core +1 more source
Understanding In Vivo Fate of Nucleic Acid and Gene Medicines for the Rational Design of Drugs
Pharmaceutics, 2021 Nucleic acid and genetic medicines are increasingly being developed, owing to their potential to treat a variety of intractable diseases. A comprehensive understanding of the in vivo fate of these agents is vital for the rational design, discovery, and ...
Shintaro Fumoto +6 more
doaj +1 more source
To transduce a zebra finch: interrogating behavioral mechanisms in a model system for speech. [PDF]
, 2017 The ability to alter neuronal gene expression, either to affect levels of endogenous molecules or to express exogenous ones, is a powerful tool for linking brain and behavior. Scientists continue to finesse genetic manipulation in mice.
Heston, Jonathan B, White, Stephanie A
core +1 more source
Carbohydrate receptor-mediated gene transfer to human T leukaemic cells [PDF]
, 1994 The mucin-type carbohydrate Tn cryptantigen (GalNAcα1-O-Ser/Thr, where GalNAc is N-acetyl-D-galactosamine) is expressed in many carcinomas, in haemopoietic disorders including the Tn syndrome, and on human immunodeficiency virus (HIV) coat glycoproteins,
Berger, Eric G. +8 more
core +2 more sources
Lack of evidence for decreased protein stability in the 2397 (Met) haplotype of the leucine rich repeat kinase 2 protein implicated in Parkinson’s disease [PDF]
, 2017 Missense mutations in the leucine rich repeat kinase 2 (LRRK2) gene are the leading genetic cause of autosomal dominant familial Parkinson’s disease.
Anderton, Ryan S +5 more
core +2 more sources