Results 21 to 30 of about 554,940 (322)
Molecular Therapy: Nucleic Acids, 2017 RNA interference (RNAi)-based strategies that mediate the specific knockdown of target genes by administration of small interfering RNAs (siRNAs) could be applied for treatment of presently incurable neurodegenerative diseases such as Parkinson’s disease.
Christin Helmschrodt +9 more
doaj +1 more source
Nucleofection as an Efficient Method for Alpha TC1-6 Cell Line Transfection
Applied Sciences, 2022 An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there is a need for an optimized transfection process for individual model systems separately.
Marija Đorđević +11 more
doaj +1 more source
Characterizing neuromorphologic alterations with additive shape functionals [PDF]
Eur. Phys. J. B 37, 109 115 (2004), 2004 The complexity of a neuronal cell shape is known to be related to its function. Specifically, among other indicators, a decreased complexity in the dendritic trees of cortical pyramidal neurons has been associated with mental retardation. In this paper we develop a procedure to address the characterization of morphological changes induced in cultured ...
arxiv +1 more source
Understanding In Vivo Fate of Nucleic Acid and Gene Medicines for the Rational Design of Drugs
Pharmaceutics, 2021 Nucleic acid and genetic medicines are increasingly being developed, owing to their potential to treat a variety of intractable diseases. A comprehensive understanding of the in vivo fate of these agents is vital for the rational design, discovery, and ...
Shintaro Fumoto +6 more
doaj +1 more source
Corticosteroid Activation of Atlantic Sea Lamprey Corticoid Receptor: Allosteric Regulation by the N-terminal Domain [PDF]
arXiv, 2022 Lampreys are jawless fish that evolved about 550 million years ago at the base of the vertebrate line. Modern lampreys contain a corticoid receptor (CR), the common ancestor of the glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), which first appear in cartilaginous fish, such as sharks.
arxiv
Haunted Bodies: Cell Switching, Getting Lost and Adaptive Geographies [PDF]
Avant, 2017 This article proposes the ideas of stochastic resonance and noise as devices with which to think of the body or self as plural and porous. Boundaries and surfaces are proposed as indefinite; cell switching and narratives of the self are discussed in ...
Jane Grant, Joanne “Bob” Whalley
doaj +1 more source
Tetraethylenepentamine-Coated β Cyclodextrin Nanoparticles for Dual DNA and siRNA Delivery
Pharmaceutics, 2022 Nucleic acid reagents, including plasmid-encoded genes and small interfering RNA (siRNA), are promising tools for validating gene function and for the development of therapeutic agents.
Chi-Hsien Liu +5 more
doaj +1 more source
Pharmaceutics, 2021 Ample evidence exists on the role of interleukin-12 (IL-12) in the response against many pathogens, as well as on its remarkable antitumor properties. However, the unexpected toxicity and disappointing results in some clinical trials are prompting the ...
Natalia Sánchez-Arribas +6 more
doaj +1 more source
Flow Cytometry Quantification of Transient Transfections in Mammalian Cells [PDF]
arXiv, 2023 Flow cytometry is a powerful quantitative assay supporting high-throughput collection of single-cell data with a high dynamic range. For flow cytometry to yield reproducible data with a quantitative relationship to the underlying biology, however, requires that 1) appropriate process controls are collected along with experimental samples, 2) these ...
arxiv
Transitions between distinct compaction regimes in complexes of multivalent cationic lipids and DNA [PDF]
Biophys. J. 95: 836 (2008), 2008 We use X-ray scattering and molecular simulations to investigate the structural properties of complexes of multivalent cationic lipids and DNA molecules. At low mole fraction of neutral lipids (NLs), $\Phi_{\rm NL}$, the complexes show dramatic DNA compaction down to essentially close packed DNA arrays with a DNA interaxial spacing $d_{\rm DNA}=25\AA$.
arxiv +1 more source