Results 21 to 30 of about 932,327 (388)
Single cell electroporation on chip [PDF]
, 2006 In this thesis the results of the development of microfluidic cell trap devices for single cell electroporation are described, which are to be used for gene transfection.
Valero, Ana
core +14 more sources
Chinese Journal of Contemporary Neurology and Neurosurgery, 2021 Objective To investigate the anti⁃tumoral effect of ACT001, a novel antitumor compound, on the nuclear factor⁃κB (NF⁃κB) pathway and programmed cell death protein ligand 1 (PDL1) expression in U87 glioblastoma cell line.
ZHANG Jin⁃hao +9 more
doaj +1 more source
A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing
Theranostics, 2020 Gene editing is a versatile technique in biomedicine that promotes fundamental research as well as clinical therapy. The development of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) as a genome editing machinery has accelerated the ...
A. K. Fajrial +4 more
semanticscholar +1 more source
Frontiers in Genome Editing, 2021 Field cress (Lepidium campestre) is a potential oilseed crop that has been under domestication in recent decades. CRISPR/Cas9 is a powerful tool for rapid trait improvement and gene characterization and for generating transgene-free mutants using ...
Sjur Sandgrind +4 more
doaj +1 more source
Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent [PDF]
Methods and Protocols, 2020 Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection efficiency is a barrier to wider realisation of the potential this model system has to offer.
Sara E. Bodbin +2 more
openaire +3 more sources
Annexin A7 and Its Related Protein Suppressor of Death Domains Regulates Migration and Proliferation of Hca-P Cells [PDF]
Cell Journal, 2023 Objective: This study was to investigate whether annexin A7 (AnnexinA7, ANXA7) and its co-related protein tumor celldeath domain silencer [suppressor of death domains (SODD)] regulates the migratory phenotype of liver cancer cells.Materials and Methods ...
Shaoqing Wang +5 more
doaj +1 more source
Infectious viruses from transfected SARS-CoV-2 genomic RNA
Frontiers in Bioengineering and Biotechnology, 2023 SARS-CoV-2 emerged at the end of 2019, and like other novel pathogens causing severe symptoms, WHO recommended heightened biosafety measures for laboratories working with the virus.
Elina Väisänen +7 more
doaj +1 more source
Cationic lipid-mediated intracellular delivery of antibodies into live cells
BioTechniques, 2008 The ability to introduce antibodies to live cells opens new insights to a wide range of applications, such as protein intracellular trafficking studies, protein interference studies with blocking antibodies, and live immunolabeling or protein ...
Claire O. Weill +2 more
doaj +1 more source
Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles. [PDF]
, 2018 PurposeThe delivery of transgenes into human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hiPSC-CMs) represents an important tool in cardiac regeneration with potential for clinical applications.
Chavez, Karen S +11 more
core +2 more sources
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Proceedings of the National Academy of Sciences of the United States of America, 1987 A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-
P. Felgner +8 more
semanticscholar +1 more source