Results 21 to 30 of about 585,058 (294)

Functional differences between protamine preparations for the transfection of mRNA

Drug Delivery, 2020
Protamine is a natural cationic peptide mixture used as a drug for the neutralization of heparin and in formulations of slow-release insulin. In addition, Protamine can be used for the stabilization and delivery of nucleic acids (antisense, small ...
Natalia Teresa Jarzebska, Severin Lauchli, Christoph Iselin, Lars E French, Pal Johansen, Emmanuella Guenova, Thomas M Kündig, Steve Pascolo   +7 more
doaj   +1 more source

ACT001 reduced the expression of programmed cell death protein ligand 1 in glioblastoma cells by inhibiting P65 phosphorylation

Chinese Journal of Contemporary Neurology and Neurosurgery, 2021
Objective To investigate the anti⁃tumoral effect of ACT001, a novel antitumor compound, on the nuclear factor⁃κB (NF⁃κB) pathway and programmed cell death protein ligand 1 (PDL1) expression in U87 glioblastoma cell line.
ZHANG Jin⁃hao, LIU Pei⁃dong, ZHANG Chen, LI Jia⁃bo, REN Xiao, CHEN Lu⁃lu, SUN Jin⁃zhang, WANG Xu⁃ya, ZHANG Liang, YANG Xue⁃jun   +9 more
doaj   +1 more source

Chitosan-Based Polymeric Nanoparticles as an Efficient Gene Delivery System to Cross Blood Brain Barrier: In Vitro and In Vivo Evaluations

Pharmaceuticals
Significant progress has been made in the field of gene therapy, but effective treatments for brain tumors remain challenging due to their complex nature.
Ishaq N. Khan, Shiza Navaid, Walifa Waqar, Deema Hussein, Najeeb Ullah, Muhammad Umar Aslam Khan, Zakir Hussain, Aneela Javed   +7 more
doaj   +1 more source

Tetraethylenepentamine-Coated β Cyclodextrin Nanoparticles for Dual DNA and siRNA Delivery

Pharmaceutics, 2022
Nucleic acid reagents, including plasmid-encoded genes and small interfering RNA (siRNA), are promising tools for validating gene function and for the development of therapeutic agents.
Chi-Hsien Liu, Pei-Yin Shih, Cheng-Han Lin, Yi-Jun Chen, Wei-Chi Wu, Chun-Chao Wang   +5 more
doaj   +1 more source

Effect of lysogeny on transfection and transfection enhancement in Bacillus subtilis [PDF]

Journal of Bacteriology, 1975
Strains of Bacillus subtilis 168 lysogenic for bacteriophage phi105 transfer with deoxyribonucleic acid (DNA) isolated from bacteriophage SPO2 at a higher efficiency than non-lysogenic strains. This enhancement of transfection was not the result of recombination between bacteriophages SPO2 and phi105. Superinfection marker rescue increased transfection
R E, Yasbin, G A, Wilson, F E, Young
openaire   +2 more sources

CCT4 promotes tunneling nanotube formation

FEBS Letters, EarlyView.
Tunneling nanotubes (TNTs) are membranous tunnel‐like structures that transport molecules and organelles between cells. They vary in thickness, and thick nanotubes often contain microtubules in addition to actin fibers. We found that cells expressing monomeric CCT4 generate many thick TNTs with tubulin.
Miyu Enomoto, Akiko Asada, Taro Saito, Kanae Ando   +3 more
wiley   +1 more source

Polyethylenimine Nanoparticle-Mediated siRNA Delivery to Reduce α-Synuclein Expression in a Model of Parkinson’s Disease

Molecular Therapy: Nucleic Acids, 2017
RNA interference (RNAi)-based strategies that mediate the specific knockdown of target genes by administration of small interfering RNAs (siRNAs) could be applied for treatment of presently incurable neurodegenerative diseases such as Parkinson’s disease.
Christin Helmschrodt, Sabrina Höbel, Sandra Schöniger, Anne Bauer, Jana Bonicelli, Marieke Gringmuth, Simone A. Fietz, Achim Aigner, Angelika Richter, Franziska Richter   +9 more
doaj   +1 more source

Nucleofection as an Efficient Method for Alpha TC1-6 Cell Line Transfection

Applied Sciences, 2022
An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there is a need for an optimized transfection process for individual model systems separately.
Marija Đorđević, Verica Paunović, Maja Jovanović Tucović, Anja Tolić, Jovana Rajić, Svetlana Dinić, Aleksandra Uskoković, Nevena Grdović, Mirjana Mihailović, Ivanka Marković, Jelena Arambašić Jovanović, Melita Vidaković   +11 more
doaj   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Enhancing nucleic acid delivery by the integration of artificial intelligence into lipid nanoparticle formulation

Frontiers in Medical Technology
The advent of messenger RNA (mRNA) therapeutics has revolutionized medicine, with its potential underscored by rapid advancements during the COVID-19 pandemic.
Kagya Amoako, Amir Mokhammad, Afrida Malik, Sumith Yesudasan, Anas Wheba, Oluwanifemi Olagunju, Sean X. Gu, Sean X. Gu, Timur Yarovinsky, Edward Vincent S. Faustino, Juliane Nguyen, John Hwa   +11 more
doaj   +1 more source