Results 31 to 40 of about 554,940 (322)

Protamine-Based Strategies for RNA Transfection

Pharmaceutics, 2021
Protamine is a natural cationic peptide mixture mostly known as a drug for the neutralization of heparin and as a compound in formulations of slow-release insulin.
Natalia Teresa Jarzebska, Mark Mellett, Julia Frei, Thomas M. Kündig, Steve Pascolo   +4 more
doaj   +1 more source

Predicting Transiently Expressed Protein Yields: Comparison of Transfection Methods in CHO and HEK293

Pharmaceutics, 2022
Therapeutic proteins are currently at the apex of innovation in pharmaceutical medicine. However, their industrial production is technically challenging and improved methods for transient transfection of mammalian cell cultures are necessary. We aimed to
Ly Porosk, Jekaterina Nebogatova, Heleri Heike Härk, Birgit Vunk, Piret Arukuusk, Urve Toots, Mart Ustav, Ülo Langel, Kaido Kurrikoff   +8 more
doaj   +1 more source

Flavonoids Enhance Lipofection Efficiency and Ameliorate Cytotoxicity in Colon26 and HepG2 Cells via Oxidative Stress Regulation

Pharmaceutics, 2022
The generation of reactive oxygen species (ROS) can affect cationic liposome-mediated transfection. In this study, we focused on a specific class of antioxidants, flavonoids, to investigate the transfection efficiency using cationic liposome/plasmid DNA ...
Die Hu, Shintaro Fumoto, Hirotaka Miyamoto, Masakazu Tanaka, Koyo Nishida   +4 more
doaj   +1 more source

Effects of PSMA1 on the differentiation and lipid deposition of bovine preadipocytes

Revista Brasileira de Zootecnia
In this study, our goal was to clarify the role of proteasomal subunit α-1 (PSMA1) in both the differentiation of preadipocytes and the accumulation of lipids in adipocytes.
Sheng Li, Chengzhen Chen, Hao Jiang, Jiabao Zhang, Qian Zhou, Yan Gao, Bao Yuan, Mingjun Zhang   +7 more
doaj   +1 more source

On‐treatment dynamics of circulating extracellular vesicles in the first‐line setting of patients with advanced non‐small cell lung cancer: the LEXOVE prospective study

Molecular Oncology, EarlyView.
The LEXOVE prospective study evaluated plasma cell‐free extracellular vesicle (cfEV) dynamics using Bradford assay and dynamic light scattering in metastatic non‐small cell lung cancer patients undergoing first‐line treatments, correlating a ∆cfEV < 20% with improved median progression‐free survival in responders versus non‐responders.
Valerio Gristina, Viviana Bazan, Nadia Barraco, Simona Taverna, Mauro Manno, Samuele Raccosta, Anna Paola Carreca, Marco Bono, Tancredi Didier Bazan Russo, Francesco Pepe, Pasquale Pisapia, Lorena Incorvaia, Giuseppe Badalamenti, Giancarlo Troncone, Umberto Malapelle, Daniele Santini, Antonio Russo, Antonio Galvano   +17 more
wiley   +1 more source

Novel Antitumor Strategy Utilizing a Plasmid Expressing a Mycobacterium tuberculosis Antigen as a “Danger Signal” to Block Immune Escape of Tumor Cells

Pharmaceutics, 2015
Immune escape of tumor cells is one of the main obstacles hindering the effectiveness of cancer immunotherapy. We developed a novel strategy to block immune escape by transfecting tumor cells in vivo with genes of pathogenic antigens from Mycobacterium ...
Yoshiyuki Koyama, Chieko Yoshihara, Tomoko Ito   +2 more
doaj   +1 more source

Effect of lysogeny on transfection and transfection enhancement in Bacillus subtilis [PDF]

Journal of Bacteriology, 1975
Strains of Bacillus subtilis 168 lysogenic for bacteriophage phi105 transfer with deoxyribonucleic acid (DNA) isolated from bacteriophage SPO2 at a higher efficiency than non-lysogenic strains. This enhancement of transfection was not the result of recombination between bacteriophages SPO2 and phi105. Superinfection marker rescue increased transfection
Frank E. Young, G A Wilson, R E Yasbin
openaire   +3 more sources

Combined spatially resolved metabolomics and spatial transcriptomics reveal the mechanism of RACK1‐mediated fatty acid synthesis

Molecular Oncology, EarlyView.
The authors analyzed the spatial distributions of gene and metabolite profiles in cervical cancer through spatial transcriptomic and spatially resolved metabolomic techniques. Pivotal genes and metabolites within these cases were then identified and validated.
Lixiu Xu, Jinqiu Li, Junqi Ma, Ayshamgul Hasim   +3 more
wiley   +1 more source

Effect of Recombinant Plasmid pEGFP-AFP-hTNF on Liver Cancer Cells (HepG2 Cells) in vitro when Delivered by PEG-PEI/Fe3O4 Nanomagnetic Fluid

Journal of the Formosan Medical Association, 2011
Gene delivery into liver cancer cells has been a problem. This study aimed to understand the effect of using PEGI/Fe3O4 nanomagnetic fluid as a gene vector for liver cancer gene therapy.
Baoxiong Zhuang, Yangde Zhang, Jian Peng, Haowei Zhang, Tiehui Hu, Jie Zeng, Yifan Li   +6 more
doaj   +1 more source

Meso-scale computer modeling of lipid-DNA complexes for gene therapy [PDF]

Physical Review Letters Vol.96, 018102 (2006), 2005
We report on a molecular simulation method which captures the self-assembly of cationic lipid-DNA (CL-DNA) gene delivery complexes. Computational efficiency required for large length- and time-scale simulations is achieved through a coarse-grained representation of the intra-molecular details, and via inter-molecular potentials, which effectively mimic
arxiv   +1 more source