Delivery of mixed-lineage kinase domain-like protein by vapor nanobubble photoporation induces necroptotic-like cell death in tumor cells [PDF]
, 2019 Modern molecular medicine demands techniques to efficiently deliver molecules directly into mammalian cells. As proteins are the final mediators of most cellular pathways, efficient intracellular protein delivery techniques are highly desired.
Braeckmans, Kevin +10 more
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Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]
, 2014 Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R +3 more
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Frontiers in Medical TechnologyThe advent of messenger RNA (mRNA) therapeutics has revolutionized medicine, with its potential underscored by rapid advancements during the COVID-19 pandemic.
Kagya Amoako +11 more
doaj +1 more source
New amphiphilic amino acid derivatives for efficient DNA transfection in vitro [PDF]
, 2017 Nucleic acids-based therapies have recently developed as next-generationagents for treating and preventing viral infection, cancer, and genetic disorders,but their use is still limited due to its relatively poor delivery into targetedcells.
Antuña, Sebastián +7 more
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PharmaceuticalsSignificant progress has been made in the field of gene therapy, but effective treatments for brain tumors remain challenging due to their complex nature.
Ishaq N. Khan +7 more
doaj +1 more source
DNMT3B in vitro knocking-down is able to reverse embryonal rhabdomyosarcoma cell phenotype through inhibition of proliferation and induction of myogenic differentiation [PDF]
, 2016 Aberrant DNA methylation has been frequently observed in many human cancers, including rhabdomyosarcoma (RMS), the most common soft tissue sarcoma in children.
Camero, Simona +11 more
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Transfection of Platyhelminthes [PDF]
BioMed Research International, 2015 Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans.
Moguel, Bárbara +3 more
openaire +2 more sources
Tetraethylenepentamine-Coated β Cyclodextrin Nanoparticles for Dual DNA and siRNA Delivery
Pharmaceutics, 2022 Nucleic acid reagents, including plasmid-encoded genes and small interfering RNA (siRNA), are promising tools for validating gene function and for the development of therapeutic agents.
Chi-Hsien Liu +5 more
doaj +1 more source
Nucleofection as an Efficient Method for Alpha TC1-6 Cell Line Transfection
Applied Sciences, 2022 An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there is a need for an optimized transfection process for individual model systems separately.
Marija Đorđević +11 more
doaj +1 more source
Headgroup modification of cholesterol-based cationic lipids: Synthesis, transfection efficiency evaluation, and serum compatibility [PDF]
Songklanakarin Journal of Science and Technology (SJST), 2020 There were two objectives for this study. First, it focused on eight lipids having cholesterol as hydrophobic tails, carbamate linkers, and different polar headgroups.
Chopaka Thongbamrer +9 more
doaj +1 more source