Results 1 to 10 of about 8,066 (202)

A structure-based high-efficiency homogeneous antibody platform by endoglycosidase Sz provides insights into its transglycosylation mechanism [PDF]

Structural Dynamics
Monoclonal antibodies (mAbs) have gradually dominated the drug markets for various diseases. Improvement of the therapeutic activities of mAbs has become a critical issue in the pharmaceutical industry.
Chun-Jung Chen
doaj   +2 more sources

Structure–Property Relevance of Two Pairs of Isomeric Steviol Rebaudiosides and the Underlying Mechanism [PDF]

Foods
Although enormous efforts have been made to prepare tasty and soluble steviol glycosides (SGs), the structure–property relationship of SGs still remains unclear, neither in experiment fact nor in the mechanism, such as the influence of linkage type and ...
Zhuoyu Zhou, Wanjie Wang, Qinbing Guo, Haijun Wang, Yongmei Xia   +4 more
doaj   +2 more sources

Characterization of a β-Galactosidase from Kosakonia oryzendophytica and Its Heterologous Expression in Bacillus subtilis for Galactooligosaccharides Production [PDF]

Molecules
Galactooligosaccharides (GOS) typically consist of 2-8 D-galactose units linked together, terminating in a D-glucose unit. GOS are commonly used in dairy products, infant formulas, and functional foods. GOS offer beneficial properties for food processing,
Zhuo Cheng, Xiangpeng Jin, Yulei Zhang, Dawei Ni, Yingying Zhu, Wei Xu, Wenli Zhang, Wanmeng Mu   +7 more
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Advancements in the Heterologous Expression of Sucrose Phosphorylase and Its Molecular Modification for the Synthesis of Glycosylated Products [PDF]

Molecules
Sucrose phosphorylase (SPase), a member of the glycoside hydrolase GH13 family, possesses the ability to catalyze the hydrolysis of sucrose to generate α-glucose-1-phosphate and can also glycosylate diverse substrates, showcasing a wide substrate ...
Hongyu Zhang, Leting Zhu, Zixuan Zhou, Danyun Wang, Jinshan Yang, Suying Wang, Tingting Lou   +6 more
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Improvement of the Transglycosylation Efficiency of a Lacto-N-Biosidase from Bifidobacterium bifidum by Protein Engineering

Applied Sciences, 2021
The lacto-N-biosidase LnbB from Bifidobacterium bifidum JCM 1254 was engineered to improve its negligible transglycosylation efficiency with the purpose of enzymatically synthesizing lacto-N-tetraose (LNT; Gal-β1,3-GlcNAc-β1,3-Gal-β1,4-Glc) in one ...
Marlene Vuillemin, Jesper Holck, Martin Matwiejuk, Eduardo S. Moreno Prieto, Jan Muschiol, Dora Molnar-Gabor, Anne S. Meyer, Birgitte Zeuner   +7 more
doaj   +1 more source

Comprehensive study on transglycosylation of CGTase from various sources

Heliyon, 2021
Transglycosylation is the in-vivo or in-vitro process of transferring glycosyl groups from a donor to an acceptor, which is usually performed by enzymatic reactions because of their simplicity, low steric hindrance, high region-specificity, low ...
Chin Hui Lim, Babak Rasti, Joko Sulistyo, Mansoor Abdul Hamid   +3 more
doaj   +1 more source

Engineering the GH1 β-glucosidase from Humicola insolens: Insights on the stimulation of activity by glucose and xylose. [PDF]

PLoS ONE, 2017
The activity of the GH1 β-glucosidase from Humicola insolens (Bglhi) against p-nitrophenyl-β-D-glucopyranoside (pNP-Glc) and cellobiose is enhanced 2-fold by glucose and/or xylose.
Luana Parras Meleiro, José Carlos Santos Salgado, Raquel Fonseca Maldonado, Sibeli Carli, Luiz Alberto Beraldo Moraes, Richard John Ward, João Atílio Jorge, Rosa Prazeres Melo Furriel   +7 more
doaj   +1 more source

Manipulation of an α-glucosidase in the industrial glucoamylase-producing Aspergillus niger strain O1 to decrease non-fermentable sugars production and increase glucoamylase activity

Frontiers in Microbiology, 2022
Dextrose equivalent of glucose from starch hydrolysis is a critical index for starch-hydrolysis industry. Improving glucose yield and decreasing the non]-fermentable sugars which caused by transglycosylation activity of the enzymes during the starch ...
Wenzhu Guo, Wenzhu Guo, Dandan Liu, Dandan Liu, Dandan Liu, Jingen Li, Jingen Li, Wenliang Sun, Wenliang Sun, Tao Sun, Tao Sun, Xingji Wang, Kefen Wang, Qian Liu, Qian Liu, Chaoguang Tian, Chaoguang Tian   +16 more
doaj   +1 more source

The characterisation of an alkali-stable maltogenic amylase from Bacillus lehensis G1 and improved malto-oligosaccharide production by hydrolysis suppression. [PDF]

PLoS ONE, 2014
A maltogenic amylase (MAG1) from alkaliphilic Bacillus lehensis G1 was cloned, expressed in Escherichia coli, purified and characterised for its hydrolysis and transglycosylation properties. The enzyme exhibited high stability at pH values from 7.0 to 10.
Nor Hasmaliana Abdul Manas, Samson Pachelles, Nor Muhammad Mahadi, Rosli Md Illias   +3 more
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Enzymatic transglycosylation for glycoconjugate synthesis [PDF]

Current Opinion in Chemical Biology, 2009
Remarkable advances have been made in recent years in exploiting the transglycosylation activity of glycosidases and glycosynthase mutants for oligosaccharide and glycoconjugate synthesis. New glycosynthases were generated from retaining glycosidases, inverting glycosidases, and those that proceed in a mechanism of substrate-assisted catalysis ...
Lai-Xi, Wang, Wei, Huang
openaire   +2 more sources