Results 11 to 20 of about 8,066 (202)

Efficient Preparation of Homogenous Antibody Conjugates via Glycosite-Specific Transglycosylation Enabled by Readily Available Glycosyl Donors. [PDF]

Angew Chem Int Ed Engl
A streamlined platform for glycosite‐specific antibody conjugation is enabled by LacNAc‐derived cyclic acetal donors, which can be prepared in only two steps and used directly in single‐enzyme Endo S2‐mediated transglycosylation. The platform supports the broad construction of homogeneous gsADCs and gsDACs, with donor 7 producing glycoengineered ...
Cai D, Zhao Y, Lu G, Li C, Lao Y, Mudududdla R, Zhang J, Jia P, Huang P, Wu W, Nguyen TT, Huang X, Li L, Tang W.   +13 more
europepmc   +3 more sources

Development of Synthetic Routes to 2'-O,4'-C-Spirocyclopentylene-Bridged Nucleic Acids: Thymidine, Guanosine, and Adenosine. [PDF]

Chemistry
A novel synthetic route to 2′‐O,4′‐C‐spirocyclopentylene‐bridged nucleic acid (scpBNA2) monomers bearing thymine, guanine, and adenine nucleobases. The strategy integrates 2′,4′‐lactonization, transglycosylation, and intramolecular iodocyclization, thereby bypassing the 2′‐OH inversion step and providing scpBNA2 nucleoside building blocks for antisense
Kumagai R, Yamada R, Yamaguchi T, Obika S.   +3 more
europepmc   +2 more sources

Structural Insight into a Yeast Maltase—The BaAG2 from Blastobotrys adeninivorans with Transglycosylating Activity

Journal of Fungi, 2021
An early-diverged yeast, Blastobotrys (Arxula) adeninivorans (Ba), has biotechnological potential due to nutritional versatility, temperature tolerance, and production of technologically applicable enzymes. We have biochemically characterized from the Ba
Karin Ernits, Christian Kjeldsen, Karina Persson, Eliis Grigor, Tiina Alamäe, Triinu Visnapuu   +5 more
doaj   +1 more source

Efficient Biocatalytic Synthesis of Dihalogenated Purine Nucleoside Analogues Applying Thermodynamic Calculations [PDF]

, 2020
The enzymatic synthesis of nucleoside analogues has been shown to be a sustainable and efficient alternative to chemical synthesis routes. In this study, dihalogenated nucleoside analogues were produced by thermostable nucleoside phosphorylases in ...
Giessmann, Robert T., Kaspar, Felix, Klare, Hendrik F. T., Kurreck, Jens, Neubauer, Peter, Paulick, Katharina, Röhrs, Viola, Wagner, Anke, Westarp, Sarah, Yehia, Heba   +9 more
core   +1 more source

Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities

Molecules, 2023
Chitooligosaccharides (COSs) are b-1,4-linked homo-oligosaccharides of N-acetylglucosamine (GlcNAc) or glucosamine (GlcN), and also include hetero-oligosaccharides composed of GlcNAc and GlcN.
Reeba Thomas, Tamo Fukamizo, Wipa Suginta   +2 more
doaj   +1 more source

Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification. [PDF]

PLoS ONE, 2018
The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies. By remodeling N-glycan to obtain a homogeneous glycoform or chemically modified glycan, antibody characteristics can be controlled or ...
Mitsuhiro Iwamoto, Yukiko Sekiguchi, Kensuke Nakamura, Yoshirou Kawaguchi, Takeshi Honda, Jun Hasegawa   +5 more
doaj   +1 more source

Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A. [PDF]

PLoS ONE, 2009
Endo-beta-N-acetylglucosaminidases (ENGases) are dual specificity enzymes with an ability to catalyze hydrolysis and transglycosylation reactions. Recently, these enzymes have become the focus of intense research because of their potential for synthesis ...
Jie Yin, Lei Li, Neil Shaw, Yang Li, Jing Katherine Song, Wenpeng Zhang, Chengfeng Xia, Rongguang Zhang, Andrzej Joachimiak, Hou-Cheng Zhang, Lai-Xi Wang, Zhi-Jie Liu, Peng Wang   +12 more
doaj   +1 more source

An Integrated Enzymatic Approach to Produce Pentyl Xylosides and Glucose/Xylose Laurate Esters From Wheat Bran

Frontiers in Bioengineering and Biotechnology, 2021
Alkyl glycosides and sugars esters are non-ionic surfactants of interest for various applications (cosmetics, food, detergency,…). In the present study, xylans and cellulose from wheat bran were enzymatically converted into pentyl xylosides and glucose ...
Chloé Jocquel, Murielle Muzard, Richard Plantier-Royon, Caroline Rémond   +3 more
doaj   +1 more source

Crystal structure of endo-β-N-acetylglucosaminidase HSα. [PDF]

Acta Crystallogr F Struct Biol Commun
Structural analysis of the GH85 enzyme endo‐β‐N‐acetylglucosaminidase HSα reveals a novel domain that is potentially involved in complex N‐glycan recognition.The crystal structure of endo‐β‐N‐acetylglucosaminidase HSα (Endo HSα) was determined at 1.8 Å resolution, revealing that the enzyme is composed of five distinct domains.
Kurauchi I, Okura K, Hosokawa C, Ito K, Miyahara I.   +4 more
europepmc   +2 more sources

Enzymatic Preparation of Gentiooligosaccharides by a Thermophilic and Thermostable β-Glucosidase at a High Substrate Concentration

Foods, 2022
Gentiooligosaccharides (GnOS) are a kind of oligosaccharide formed by glucose with β-1-6 glycosidic bonds, which has become a new type of functional oligosaccharide for its unique refreshing bitter taste and valuable probiotic effects.
Wei Xia, Lingling Sheng, Wanmeng Mu, Yuping Shi, Jing Wu   +4 more
doaj   +1 more source