A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses. [PDF]
J Vis Exp, 2018 Wu F, Hanzawa Y.
europepmc +1 more source
Agrobacterium-Mediated Transient Gene Expression in Developing Ricinus communis Seeds: A First Step in Making the Castor Oil Plant a Chemical Biofactory. [PDF]
Front Plant Sci, 2019 Sánchez-Álvarez A +5 more
europepmc +1 more source
Transient gene expression in electroporated Solanum protoplasts
Plant Molecular Biology, 1989 Electroporation was used to evaluate parameters important in transient gene expression in potato protoplasts. The protoplasts were from leaves of wild potato Solanum brevidens, and from leaves, tubers and suspension cells of cultivated Solanum tuberosum cv. Désirée. Reporter enzyme activity, chloramphenicol acetyl transferase (CAT) under the control of
Jones, H., Ooms, G., Jones, M.G.K.
openaire +3 more sources
Increasing Transfection Efficiency of Lipoplexes by Modulating Complexation Solution for Transient Gene Expression [PDF]
International Journal of Molecular Sciences, 2021 Kim Jaemun, Eun Gyô Lee
exaly +2 more sources
An Efficient and Universal Protoplast Isolation Protocol Suitable for Transient Gene Expression Analysis and Single-Cell RNA Sequencing [PDF]
International Journal of Molecular Sciences, 2022 Xia Yang, Yang Dong, Juanjuan Wang
exaly +2 more sources
Related searches:
Transient gene expression in untransformed lymphocytes
European Journal of Immunology, 1987AbstractConditions for transfection of normal, freshly isolated and mitogen‐stimulated spleen cells were established. A sharp optimum of plasmid at 10 μg per 1.5 × 107 transfected spleen cells per transfection was observed. The Ig promoter and the IgH enhancer activity was found to be restricted to cells of the B lineage also in normal, freshly ...
I L, Mårtensson, T, Leanderson
openaire +2 more sources
Transient Gene Expression Levels from Multigene Expression Vectors
Biotechnology Progress, 2007AbstractMultigene expression vectors are commonly utilized whereby the (over)expression of two or more genes is desired simultaneously and often at supposedly equivalent levels. We have characterized dual‐gene and pEE14.4 RlucMFluc expression plasmids in which the second hCMV‐MIE promoter is replaced with a c‐myc IRES to enable one‐step coordinated ...
Underhill, MF +4 more
openaire +5 more sources
Transient Gene Expression in Human Expi293 Cells
2022Transient gene expression (TGE) is an important tool for generating recombinant proteins in a short period of time. The human cell line HEK293 is widely used for this purpose since it can grow in suspension to a high cell density in serum-free media.
openaire +2 more sources