Results 141 to 150 of about 5,757,325 (187)
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Variation in enzymatic transient gene expression assays
Analytical Biochemistry, 1989We examined causes for high variability in data from enzymatic transient gene expression assays. Our results strongly suggest that variation in transfection efficiency is the major cause of data variation and can seriously compromise valid interpretation of data.
T, Hollon, F K, Yoshimura
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Methods for transient and stable expression of heterologous genes in Physarum
Cell Biology International Reports, 1992103351
Burland, T. +4 more
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Transient Gene Expression of Chimeric Genes in Cells and Tissues of Crops
1991The recent progress in understanding plant biology at the molecular level has been due, in part, to the development of efficient gene transfer systems for many plant species. Without doubt, the most efficient way to transfer genes to crops is with Agrobacterium tumefaciens. Fraley et al.
H H, Steinbiss, A, Davidson
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Intron-dependent transient expression of the maize GapA1 gene
Plant Molecular Biology, 1995Transient expression experiments show that the maize GapA1 promoter exhibits a requirement for sequences contained within intron 1 and surrounding exon border regions for expression in maize Black Mexican Sweet cells. Maize GapA1-promoter constructs lacking intron 1 are inactive. Intron 1 and its exon border sequences, when reintroduced into constructs
M, Donath +3 more
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Transient Gene Expression in Suspension HEK293-EBNA1 Cells
2018Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection
L’Abbé, Denis +4 more
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Ubiquitin gene expression following transient forebrain ischemia
Molecular Brain Research, 1996Ubiquitin gene expression following transient forebrain ischemia in the rat was analyzed by three probes which were specific for UbC, UbB and UbS30 mRNA. According to the in situ hybridization studies, each type of ubiquitin gene expression decreased at 30 min of reperfusion following 20 min of forebrain ischemia, thereafter increased, and then reached
M, Noga, T, Hayashi
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Gene Transfer to Protoplasts: Transient Gene Expression Analysis
1995While the aim of the first transformation experiments was to obtain stably transformed transgenic plants, direct DNA uptake by plant cells has also proven extremely useful as a means to analyze rapidly the expression and regulation of specific introduced genes via transient expression analysis. To address questions regarding mRNA stability or turnover,
J. C. Carle Urioste +3 more
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Large-scale plasmid preparation for transient gene expression
Biotechnology Letters, 2011Large-scale transient gene expression of recombinant protein in mammalian cells requires a great amount of plasmid. An economical method for large-scale plasmid preparation, based on fed-batch fermentation and an improved plasmid extraction process, has been established. Fed-batch growth of E.
Lu, Cheng +3 more
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In vivo transient expression for the functional analysis of polydnaviral genes
Journal of Invertebrate Pathology, 2012Transient expression of a foreign gene in an organism is useful to determine its physiological function. This study introduces an efficient expression technique in the insect system using a recombinant eukaryotic expression vector. A recombinant construct expressing an enhanced green fluorescence protein (EGFP) gene under an immediately early promoter ...
Rahul, Hepat, Yonggyun, Kim
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Transient Expression of Homologous Genes in Drosophila Cells
Science, 1984A cloned Drosophila heat shock protein 22 gene was transfected into two independently established Drosophila cell lines. Each line carried a different heat shock protein 22 allele, distinguishable by electrophoresis of the protein.
C M, Morganelli, E M, Berger
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