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Methods for Transient Gene Expression in Wild Relatives of Potato
2018Genomics studies in potato and other plants have elucidated a large number of genes involved in a wide array of phenotypes. In particular, recent bioinformatic and genomic analyses of oomycetes and fungi have identified many effectors for which the corresponding host resistance-eliciting receptor remains to be found.
Sidrat, Abdullah, Dennis, Halterman
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Rapid reprogramming of globin gene expression in transient heterokaryons
Cell, 1986Interspecific heterokaryons were formed by fusing adult mouse erythroleukemia (MEL) cells and human embryonic/fetal erythroid (K562) cells with each other, or with a variety of mouse and human nonerythroid cell types. Analysis of total cellular RNA isolated 24 hr after fusion revealed that normally inactive globin genes can be activated in these ...
M H, Baron, T, Maniatis
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The Physcomitrella patens System for Transient Gene Expression Assays
2016Transient expression assays are valuable techniques to study in vivo the transcriptional regulation of gene expression. These methods allow to assess the transcriptional properties of a given transcription factor (TF) or a complex of regulatory proteins against specific DNA motifs, called cis-regulatory elements.
Thévenin, Johanne +5 more
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Transient gene expression in electroporated Picea glauca protoplasts
Plant Cell Reports, 1988The reporter gene for chloramphenicol acetyltransferase (CAT) was introduced into white spruce (Picea glauca (Moench) Voss.) protoplasts by electroporation. CAT transient gene expression was increased by increasing the concentration of pCaMVCN plasmid and was affected by the level of the applied voltage.
F, Bekkaoui +5 more
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Inducible Gene Expression in Transient Transgenic Xenopus Embryos
2008Xenopus laevis has for many years been successfully used to study Wnt signaling during early development. However, because loss of function and gain of function experiments generally involve injecting RNA, DNA, or morpholinos into early embryos (1- to 32-cell), major phenotypes are often observed before the embryo has reached later stages of ...
Wheeler, Grant N. +2 more
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Transient expression assay for qualitative assessment of gene expression by fowlpox virus
Virus Genes, 1990A transient expression assay for fowlpox virus (FPV) was developed to assess the feasibility of using heterologous promoters in FPV and to qualitatively determine relative promoter strength. A transient expression system for FPV has not been reported, and various methods used for transient expression in vaccinia-virus-infected cells produced negative ...
S, Dhawale, C E, Beisel, K, Nazerian
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PEI-Mediated Transient Gene Expression in CHO Cells
2018We describe a method for polyethyleneimine (PEI)-mediated transient transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells for protein expression applicable at scales from 2 mL to 2 L. The method involves transfection at a high cell density (5 × 106 cells/mL) by direct addition of plasmid DNA (pDNA) and PEI to the culture and ...
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Tissue plasminogen activator (tPA) as a reporter gene in transient gene expression
Gene, 1987Using the gene coding for tissue plasminogen activator (tPA) as a reporter gene, a transient gene expression system has been established. Vectors containing the full-length cDNA of tPA with its signal sequences were introduced into mammalian recipient cells by a modified gene transfer procedure.
S M, Cheng +5 more
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In Vitro Cellular & Developmental Biology - Plant, 1999
The influence of a variety of parameters was investigated on polyethylene glycol (PEG)-mediated transient nptII and gus gene expression in mesophyll protoplasts of Arabidopsis thaliana ecotype, Estland, in order to develop a suitable transient gene expression system.
Rita Gandhi +2 more
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The influence of a variety of parameters was investigated on polyethylene glycol (PEG)-mediated transient nptII and gus gene expression in mesophyll protoplasts of Arabidopsis thaliana ecotype, Estland, in order to develop a suitable transient gene expression system.
Rita Gandhi +2 more
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Transient gene expression in a Gramineae cell line
Molecular and General Genetics MGG, 1986We have fused the promoter of the shrunken gene isolated from Zea mays to the bacterial neomycin phosphotransferase II gene from Tn5. The plasmid construction pSKAN1 was used for transformation of a Triticum monococcum suspension cell line. Expression of the chimaeric gene was analysed a short time after transformation by estimating neomycin ...
Wolfgang Werr, Horst Lörz
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