Results 161 to 170 of about 6,113,361 (250)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Correction: An effective method to enhance transient gene expression by transfecting high-density CHO cells. [PDF]

AMB Express
Fu YS, Han XL, Li WD, Shan LL, Geng SL, Mi CL, Dong WH, Wang TY, Wang XY.   +8 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

An efficient method for tissue-specific protoplast isolation suitable for single-cell RNA sequencing and transient gene expression analysis in saffron (Crocus sativus L.). [PDF]

BMC Biotechnol
Xi X, Song J, Feng M, Qian X, Sun H, Li L.   +5 more
europepmc   +1 more source

A Rapid and Highly Efficient Method for Transient Gene Expression in Rice Plants. [PDF]

Front Plant Sci, 2020
Burman N, Chandran D, Khurana JP.
europepmc   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Transient gene expression in electroporated protoplasts and intact cells of sugar beet.

, 1987
Factors influencing the transient expression of introduced foreign DNA in electroporated protoplasts and intact cells of sugar beet were determined by assaying for the activity of chloramphenicol acetyltransferase (CAT), using a rectangular pulse ...
Jones, M.G.K., Jones, M. G. K., Lindsey, K.   +2 more
core  

Protoplast isolation and transient gene expression in Suaeda aralocaspica, a halophyte with single-cell C₄ anatomy. [PDF]

Plant Methods
Cao J, Liao X, Yu R, Asihatibieke B, Liu Y, Lan H.   +5 more
europepmc   +1 more source

Biolistic Approach for Transient Gene Expression Studies in Plants. [PDF]

Methods Mol Biol, 2020
Lacroix B, Citovsky V.
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source