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Inducible Gene Expression in Transient Transgenic Xenopus Embryos
2008Xenopus laevis has for many years been successfully used to study Wnt signaling during early development. However, because loss of function and gain of function experiments generally involve injecting RNA, DNA, or morpholinos into early embryos (1- to 32-cell), major phenotypes are often observed before the embryo has reached later stages of ...
Wheeler, Grant N. +2 more
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Gene Transfer to Protoplasts: Transient Gene Expression Analysis
1995While the aim of the first transformation experiments was to obtain stably transformed transgenic plants, direct DNA uptake by plant cells has also proven extremely useful as a means to analyze rapidly the expression and regulation of specific introduced genes via transient expression analysis. To address questions regarding mRNA stability or turnover,
J. C. Carle Urioste +3 more
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Large-scale plasmid preparation for transient gene expression
Biotechnology Letters, 2011Large-scale transient gene expression of recombinant protein in mammalian cells requires a great amount of plasmid. An economical method for large-scale plasmid preparation, based on fed-batch fermentation and an improved plasmid extraction process, has been established. Fed-batch growth of E.
Lu, Cheng +3 more
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In vivo transient expression for the functional analysis of polydnaviral genes
Journal of Invertebrate Pathology, 2012Transient expression of a foreign gene in an organism is useful to determine its physiological function. This study introduces an efficient expression technique in the insect system using a recombinant eukaryotic expression vector. A recombinant construct expressing an enhanced green fluorescence protein (EGFP) gene under an immediately early promoter ...
Rahul, Hepat, Yonggyun, Kim
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Transient Expression of Homologous Genes in Drosophila Cells
Science, 1984A cloned Drosophila heat shock protein 22 gene was transfected into two independently established Drosophila cell lines. Each line carried a different heat shock protein 22 allele, distinguishable by electrophoresis of the protein.
C M, Morganelli, E M, Berger
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Methods for Transient Gene Expression in Wild Relatives of Potato
2018Genomics studies in potato and other plants have elucidated a large number of genes involved in a wide array of phenotypes. In particular, recent bioinformatic and genomic analyses of oomycetes and fungi have identified many effectors for which the corresponding host resistance-eliciting receptor remains to be found.
Sidrat, Abdullah, Dennis, Halterman
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Transient expression assay for qualitative assessment of gene expression by fowlpox virus
Virus Genes, 1990A transient expression assay for fowlpox virus (FPV) was developed to assess the feasibility of using heterologous promoters in FPV and to qualitatively determine relative promoter strength. A transient expression system for FPV has not been reported, and various methods used for transient expression in vaccinia-virus-infected cells produced negative ...
S, Dhawale, C E, Beisel, K, Nazerian
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In Vitro Cellular & Developmental Biology - Plant, 1999
The influence of a variety of parameters was investigated on polyethylene glycol (PEG)-mediated transient nptII and gus gene expression in mesophyll protoplasts of Arabidopsis thaliana ecotype, Estland, in order to develop a suitable transient gene expression system.
Rita Gandhi +2 more
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The influence of a variety of parameters was investigated on polyethylene glycol (PEG)-mediated transient nptII and gus gene expression in mesophyll protoplasts of Arabidopsis thaliana ecotype, Estland, in order to develop a suitable transient gene expression system.
Rita Gandhi +2 more
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Tissue plasminogen activator (tPA) as a reporter gene in transient gene expression
Gene, 1987Using the gene coding for tissue plasminogen activator (tPA) as a reporter gene, a transient gene expression system has been established. Vectors containing the full-length cDNA of tPA with its signal sequences were introduced into mammalian recipient cells by a modified gene transfer procedure.
S M, Cheng +5 more
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Transient gene expression in a Gramineae cell line
Molecular and General Genetics MGG, 1986We have fused the promoter of the shrunken gene isolated from Zea mays to the bacterial neomycin phosphotransferase II gene from Tn5. The plasmid construction pSKAN1 was used for transformation of a Triticum monococcum suspension cell line. Expression of the chimaeric gene was analysed a short time after transformation by estimating neomycin ...
Wolfgang Werr, Horst Lörz
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