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PEI-Mediated Transient Gene Expression in CHO Cells
2018We describe a method for polyethyleneimine (PEI)-mediated transient transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells for protein expression applicable at scales from 2 mL to 2 L. The method involves transfection at a high cell density (5 × 106 cells/mL) by direct addition of plasmid DNA (pDNA) and PEI to the culture and ...
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Gene Expression Programming Algorithm for Transient Security Classification
2012In this paper, a gene expression programming (GEP) based algorithm is implemented for power system transient security classification. The GEP algorithms as evolutionary algorithms for pattern classification have recently received attention for classification problems because they can perform global searches. The proposed methodology applies the GEP for
Almoataz Y. Abdelaziz +4 more
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Transient Gene Expression in Sunflower Mesophyll Protoplasts
Botanica Acta, 1991AbstractExperiments on transient gene expression were performed with mesophyll protoplasts isolated from young leaves of the sunflower (Helianthus annuus L.). Both PEG‐mediated DNA transfer as well as electroporation, were shown to be suitable for the introduction of a foreign gene.
E. Kirches, N. Frey, Heide Schnabl
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Transient Gene Transfer and Expression in the Lung
Chest, 1997P J, Sime +4 more
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Transient Expression of Cloned Genes in Mammalian Cells
1983Diploid organisms do not lend themselves easily to the kind of classical genetic analysis that has led to the identification of many proteins, DNA sequences and pathways that play regulatory roles in haploid organisms. Instead, recent advances in our ability to clone individual genes, determine their primary structure and subsequently remodel them have
Julian Banerji, Walter Schaffner
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Transient gene expression in electroporated plant cells
Trends in Biotechnology, 1985Rapport du postes de Frommet al. au congres UCLA-ARCO sur la gentique des vegetaux: technique d'electroporation appliquee aux protoplastes vegetaux et ...
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A simple and efficient agroinfiltration method for transient gene expression in Citrus
Plant Cell Reports, 2021Stacy Welker +2 more
exaly
Transient Gene Expression in Protoplasts of Arabidopsis thaliana
1998S, Abel, A, Theologis
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