Results 11 to 20 of about 8,654 (179)

A δ38 deletion variant of human transketolase as a model of transketolase-like protein 1 exhibits no enzymatic activity. [PDF]

PLoS ONE, 2012
Besides transketolase (TKT), a thiamin-dependent enzyme of the pentose phosphate pathway, the human genome encodes for two closely related transketolase-like proteins, which share a high sequence identity with TKT.
Stefan Schneider, Stefan Lüdtke, Kathrin Schröder-Tittmann, Cindy Wechsler, Danilo Meyer, Kai Tittmann   +5 more
doaj   +1 more source

Enhancing erythritol production from crude glycerol in a wild-type Yarrowia lipolytica by metabolic engineering

Frontiers in Microbiology, 2022
Background: Erythritol is a zero-calorie sweetener that is widely used in the food, pharmaceutical, and medical industries. Crude glycerol is the main by-product of biodiesel, and the effective utilization of crude glycerol will help to improve biodiesel
Shuling Yang, Xuewei Pan, Qiang Wang, Qinglan Lv, Xian Zhang, Rongzhen Zhang, Zhiming Rao   +6 more
doaj   +1 more source

Inhibition of Transketolase Improves the Prognosis of Colorectal Cancer

Frontiers in Medicine, 2022
Colorectal cancer (CRC) remains a heavy health burden worldwide. Transketolase (TKT) is a crucial enzyme in the non-oxidative phase of the Pentose Phosphate Pathway (PPP), and is up-regulated in multiple cancer types.
Linhao Zhang, Linhao Zhang, Zhiyin Huang, Qiuyu Cai, Qiuyu Cai, Chong Zhao, Chong Zhao, Yang Xiao, Yang Xiao, Xin Quan, Xin Quan, Chengwei Tang, Chengwei Tang, Jinhang Gao, Jinhang Gao   +14 more
doaj   +1 more source

Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases of Bacillus methanolicus by Site-Directed Mutagenesis of Key Amino Acids and Gene Repression by CRISPR Interference

Frontiers in Microbiology, 2021
The Gram-positive Bacillus methanolicus shows plasmid-dependent methylotrophy. This facultative ribulose monophosphate (RuMP) cycle methylotroph possesses two fructose bisphosphate aldolases (FBA) with distinct kinetic properties.
Kerstin Schultenkämper, Desirée D. Gütle, Marina Gil López, Laura B. Keller, Lin Zhang, Oliver Einsle, Jean-Pierre Jacquot, Volker F. Wendisch   +7 more
doaj   +1 more source

Overexpression of transketolase-like gene 1 is associated with cell proliferation in uterine cervix cancer

Journal of Experimental & Clinical Cancer Research, 2009
Background Tumor cells need large energy and nucleic acids to proliferate and grow. For most of their energy needs, cancer cells depend more on glycolysis. For most of their nucleic acids needs, cancer cells depend more on the nonoxidative pathway of the
Chen Hui, Yue Jian-Xin, Yang Shou-Hua, Ding Hui, Zhao Rong-Wei, Zhang Song   +5 more
doaj   +1 more source

Novel transketolase inhibitor oroxylin A suppresses the non‐oxidative pentose phosphate pathway and hepatocellular carcinoma tumour growth in mice and patient‐derived organoids

Clinical and Translational Medicine, 2022
Background Transketolase (TKT), a key rate‐limiting enzyme in the non‐oxidative branch of the pentose phosphate pathway (PPP), provides more than 85% of the ribose required for de novo nucleotide biosynthesis and promotes the development of ...
Dan Jia, Chunliang Liu, Zhenyu Zhu, Yan Cao, Wen Wen, Zhanying Hong, Yue Liu, Erdong Liu, Long Chen, Chun Chen, Yanqiu Gu, Binghua Jiao, Yifeng Chai, Hong‐yang Wang, Jing Fu, Xiaofei Chen   +15 more
doaj   +1 more source

Reduced Thiamine Availability and Hyperglycemia Impair Thiamine Transport in Renal Glomerular Cells through Modulation of Thiamine Transporter 2

Biomedicines, 2021
Thiamine helps transketolase in removing toxic metabolites, counteracting high glucose-induced damage in microvascular cells, and progression of diabetic retinopathy/nephropathy in diabetic animals.
Aurora Mazzeo, Federica Barutta, Linda Bellucci, Marina Trento, Gabriella Gruden, Massimo Porta, Elena Beltramo   +6 more
doaj   +1 more source

Acidosis Activates the Nrf2 Pathway in Renal Proximal Tubule-Derived Cells through a Crosstalk with Renal Fibroblasts

Antioxidants, 2023
Crosstalk of renal epithelial cells with interstitial fibroblasts plays an important role in kidney pathophysiology. A previous study showed that crosstalk between renal epithelial cells and renal fibroblasts protects against acidosis-induced damage.
Marie-Christin Schulz, Virginie Dubourg, Alexander Nolze, Michael Kopf, Gerald Schwerdt, Michael Gekle   +5 more
doaj   +1 more source

The significance of solute carrier group of genes in the pathogenesis and treatment of diabetic microvascular complications

Journal of Education, Health and Sport, 2020
Aim- To study the impact of the solute carrier group of genes on the mechanisms involved in hyperglycemia induced tissue damage and its implication on the treatment of diabetic microvascular complications.Introduction – the estimated figure of people ...
K. Singh, T. Yuzvenko
doaj   +1 more source

New Assays for Transketolase

Bioscience, Biotechnology, and Biochemistry, 1993
Given the importance of transketolase, TK (EC 2.2.1.1) for both pharmacological studies and synthetic purposes, the need for a simple and inexpensive assay is patent. We describe here a simple and inexpensive TK assay using the unphosphorylated ketoses L-erythrulose, 4-deoxy-L-erythrulose, and 4-deoxy-D,L-erythrulose as donor substrates instead of D ...
Hecquet, Laurence, Bolte, Jean, Demuynck, Colette   +2 more
openaire   +1 more source