Results 11 to 20 of about 10,076 (212)

A δ38 deletion variant of human transketolase as a model of transketolase-like protein 1 exhibits no enzymatic activity. [PDF]

PLoS ONE, 2012
Besides transketolase (TKT), a thiamin-dependent enzyme of the pentose phosphate pathway, the human genome encodes for two closely related transketolase-like proteins, which share a high sequence identity with TKT.
Stefan Schneider, Stefan Lüdtke, Kathrin Schröder-Tittmann, Cindy Wechsler, Danilo Meyer, Kai Tittmann   +5 more
doaj   +2 more sources

Overexpression of transketolase-like gene 1 is associated with cell proliferation in uterine cervix cancer [PDF]

Journal of Experimental & Clinical Cancer Research, 2009
Background Tumor cells need large energy and nucleic acids to proliferate and grow. For most of their energy needs, cancer cells depend more on glycolysis. For most of their nucleic acids needs, cancer cells depend more on the nonoxidative pathway of the
Chen Hui, Yue Jian-Xin, Yang Shou-Hua, Ding Hui, Zhao Rong-Wei, Zhang Song   +5 more
doaj   +2 more sources

Heterologous expression of human transketolase [PDF]

The International Journal of Biochemistry & Cell Biology, 1998
Transketolase belongs to the family of thiamin diphosphate dependent enzymes. The aim of this study was to establish a bacterial expression system for human transketolase in order to investigate the functional characteristics of mammalian transketolases. The level of recombinant human enzyme expressed in Escherichia coli was modest.
Schenk, Gerhard, Duggleby, Ronald, Nixon, Peter   +2 more
openaire   +8 more sources

Inhibition of transketolase by p‐hydroxyphenylpyruvate

FEBS Letters, 1999
The effect of p‐hydroxyphenylpyruvate, a natural analogue of transketolase substrate, on the catalytic activity of the enzyme was investigated. p‐Hydroxyphenylpyruvate proved to be a reversible and competitive inhibitor of transketolase with respect to substrate; it was also able to displace thiamine diphosphate from holotransketolase. The data suggest
Solovjeva, Olga N, Kochetov, German A
openaire   +3 more sources

Transketolase-like 1 ectopic expression is associated with DNA hypomethylation and induces the Warburg effect in melanoma cells

, 2022
Background: The metabolism of cancer cells is often reprogrammed by dysregulation of metabolic enzymes. Transketolase-like 1 (TKTL1) is a homodimeric transketolase linking the pentose-phosphate pathway with the glycolytic pathway.
P-H Lo (12526261), M Davalos-Salas (12526270), Andreas Behren (9477650), AC Chueh (12526264), M Anaka (9888308), Jonathan Cebon (9483446), Aparna Jayachandran (3460802), R Molania (12526267), Marzena Walkiewicz (436361), P Prithviraj (9888305)   +9 more
core   +2 more sources

Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases of Bacillus methanolicus by Site-Directed Mutagenesis of Key Amino Acids and Gene Repression by CRISPR Interference

Frontiers in Microbiology, 2021
The Gram-positive Bacillus methanolicus shows plasmid-dependent methylotrophy. This facultative ribulose monophosphate (RuMP) cycle methylotroph possesses two fructose bisphosphate aldolases (FBA) with distinct kinetic properties.
Kerstin Schultenkämper, Desirée D. Gütle, Marina Gil López, Laura B. Keller, Lin Zhang, Oliver Einsle, Jean-Pierre Jacquot, Volker F. Wendisch   +7 more
doaj   +1 more source

Novel transketolase inhibitor oroxylin A suppresses the non‐oxidative pentose phosphate pathway and hepatocellular carcinoma tumour growth in mice and patient‐derived organoids

Clinical and Translational Medicine, 2022
Background Transketolase (TKT), a key rate‐limiting enzyme in the non‐oxidative branch of the pentose phosphate pathway (PPP), provides more than 85% of the ribose required for de novo nucleotide biosynthesis and promotes the development of ...
Dan Jia, Chunliang Liu, Zhenyu Zhu, Yan Cao, Wen Wen, Zhanying Hong, Yue Liu, Erdong Liu, Long Chen, Chun Chen, Yanqiu Gu, Binghua Jiao, Yifeng Chai, Hong‐yang Wang, Jing Fu, Xiaofei Chen   +15 more
doaj   +1 more source

Carbohydrate Metabolism Parameters of Adult Glial Neoplasms According to Immunohistochemical Profile

Biomedicines, 2022
This research aimed to investigate the interrelationship of carbohydrate metabolism parameters and immunohistochemical characteristics of glial tumors. Tumor tissue, peritumoral area, and adjacent noncancerous tissue fragments of 20 patients with gliomas
Larisa Obukhova, Olga Nikiforova, Claudia Kontorshchikova, Igor Medyanik, Natalya Orlinskaya, Artem Grishin, Michael Kontorshchikov, Natalya Shchelchkova   +7 more
doaj   +1 more source

Enhancing erythritol production from crude glycerol in a wild-type Yarrowia lipolytica by metabolic engineering

Frontiers in Microbiology, 2022
Background: Erythritol is a zero-calorie sweetener that is widely used in the food, pharmaceutical, and medical industries. Crude glycerol is the main by-product of biodiesel, and the effective utilization of crude glycerol will help to improve biodiesel
Shuling Yang, Xuewei Pan, Qiang Wang, Qinglan Lv, Xian Zhang, Rongzhen Zhang, Zhiming Rao   +6 more
doaj   +1 more source

The significance of solute carrier group of genes in the pathogenesis and treatment of diabetic microvascular complications

Journal of Education, Health and Sport, 2020
Aim- To study the impact of the solute carrier group of genes on the mechanisms involved in hyperglycemia induced tissue damage and its implication on the treatment of diabetic microvascular complications.Introduction – the estimated figure of people ...
K. Singh, T. Yuzvenko
doaj   +1 more source