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Mariner Mos1 Transposase Dimerizes Prior to ITR Binding
Journal of Molecular Biology, 2005The mariner Mos1 synaptic complex consists of a tetramer of transposase molecules that bring together the two ends of the element. Such an assembly requires at least two kinds of protein-protein interfaces. The first is involved in "cis" dimerization, and consists of transposase molecules bound side-by-side on a single DNA molecule.
Augé-Gouillou, Corinne +4 more
openaire +4 more sources
Tn5 transposase-mediated mouse transgenesis.
Biology of reproduction, 2006We have developed a novel method for mouse transgenesis. The procedure relies on a hyperactive Tn5 transposase to insert a transgene into mouse chromosomes during intracytoplasmic sperm injection. This procedure integrates foreign DNA into the mouse genome with dramatically increased effectiveness as compared to conventional methods such as pronuclear ...
Ryota, Suganuma +5 more
openaire +1 more source
Mechanism of target site selection by type V-K CRISPR-associated transposases
Science, 2023Jerrin Thomas George +2 more
exaly
Precise cut-and-paste DNA insertion using engineered type V-K CRISPR-associated transposases
Nature Biotechnology, 2023Connor J Tou +2 more
exaly
Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration
Nucleic Acids Research, 2021Siqi Yang, Jieze Zhang, Sheng Yang
exaly