Results 261 to 270 of about 11,351 (298)

Overproduction and Purification of the Tn3 Transposase

The Journal of Biochemistry, 1987
The Tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing Escherichia coli strain harboring a transposase overproducer plasmid. This accumulation of the transposase seems to be due to four factors: derepression of transcription resulting from inactivation of the repressor gene (tnpR); efficient translation caused by a ...
M, Morita, S, Tsunasawa, Y, Sugino
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Cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation

Molecular Microbiology, 1996
Summary The transposase protein encoded by the insertion element IS 903 belongs to an unusual class of DNA‐binding proteins, termed cis‐acting proteins, that act preferentially at their site of synthesis. Previous work had led us to propose that instability of the IS 903 transposase was a major determinant of its cis preference.
K M, Derbyshire, N D, Grindley
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Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery [PDF]

Nucleic Acids Research, 2011
The Sleeping Beauty (SB) transposase and its newly developed hyperactive variant, SB100X, are of increasing interest for genome modification in experimental models and gene therapy.
Melanie Galla, Axel Schambach, Christine S Falk   +2 more
exaly   +2 more sources

Transposase-driven rearrangements in human tumors

Nature Genetics, 2017
A new study shows that aberrant DNA transposase activity promotes structural alterations that are clonally selected to drive tumor development. This discovery uncovers novel mechanisms of tumor-suppressor gene inactivation and highlights a new approach to cancer gene identification.
Stephen C, Mack, Hiromichi, Suzuki, Michael D, Taylor   +2 more
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Interaction of Tn5 Transposase with the Transposon Termini

Journal of Molecular Biology, 1994
Transposition of Tn5 requires the binding of the transposase protein to the transposon outside end (OE) DNA sequences. Transposase mutants that increase the transposition frequency result in the formation of two distinct transposase/OE DNA complexes, observed by gel retardation analysis.
T W, Wiegand, W S, Reznikoff
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Evidence for “Unseen” Transposase–DNA Contacts

Journal of Molecular Biology, 2002
In this study, evidence of novel, important interactions between a hyperactive Tn5 transposon recognition end sequence and hyperactive Tn5 transposase (Tnp) are presented. A hyperactive Tn5 end sequence, the mosaic end (ME), was isolated previously.
Mindy, Steiniger-White, Archna, Bhasin, Scott, Lovell, Ivan, Rayment, William S, Reznikoff   +4 more
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DNA binding domains in Tn3 transposase

Molecular and General Genetics MGG, 1993
Various segments of Tn3 transposase were fused individually to beta-galactosidase, and the resulting fusion proteins were examined for their DNA binding ability by a nitrocellulose filter binding assay. Analyses of a series of the fusion proteins revealed that the N-terminal segment of the transposase (amino acid positions 1-242; the transposase gene ...
T, Maekawa, J, Amemura-Maekawa, E, Ohtsubo   +2 more
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Defining functional regions of the IS903 transposase

Journal of Molecular Biology, 1997
The insertion sequence IS903 encodes a 307 amino acid residue protein, transposase, that is essential for transposition. It is a multi-functional DNA-binding protein that specifically recognizes the 18 bp inverted repeats at the ends of the element and also recognizes DNa non-specifically when it captures a target site.
N P, Tavakoli, J, DeVost, K M, Derbyshire   +2 more
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Induction of the SOS Response by IS1 Transposase

Journal of Molecular Biology, 1994
We find that IS1 transposase, like that of Tn10, can induce the SOS response when produced at high levels. Most of the activity (> 80%) requires IS1 ends in cis to the transposase gene and depends strictly on the presence of RecBCD function. This implies that processing of transposase-induced cleavages is responsible for generating the response ...
D, Lane, J, Cavaillé, M, Chandler
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The role of TnA transposase in transposition immunity

Plasmid, 1984
The strength of expression of transposition immunity depends on the level of transposase available in the system, and on the location of the element to be transposed.
J, Heritage, P M, Bennett
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