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Bacterial genome engineering using CRISPR-associated transposases

Nature Protocols
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated transposases have the potential to transform the technology landscape for kilobase-scale genome engineering, by virtue of their ability to integrate large genetic payloads with high accuracy, easy programmability and no requirement for homologous recombination machinery ...
Phuc Leo H Vo, Sanne E Klompe, Carlotta Ronda   +2 more
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Chapter 11. DNA Transposases

2008
One of the greatest paradigm shifts in the early days of molecular biology was the discovery of DNA transposition by Barbara McClintock in 1948.1 Working with maize, McClintock realized that certain chromosomal elements had the ability to move from one location to another in the genome,2 an observat...
Fred Dyda, Alison Burgess Hickman
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Defining functional regions of the IS903 transposase

Journal of Molecular Biology, 1997
The insertion sequence IS903 encodes a 307 amino acid residue protein, transposase, that is essential for transposition. It is a multi-functional DNA-binding protein that specifically recognizes the 18 bp inverted repeats at the ends of the element and also recognizes DNa non-specifically when it captures a target site.
N P, Tavakoli, J, DeVost, K M, Derbyshire   +2 more
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First Mariner Mos1 transposase inhibitors.

Mini reviews in medicinal chemistry, 2009
We described chemical inhibitors of Mos1 transposition. Some were already known to affect a related prokaryotic transposase (Tn5) or HIV-1 integrase, whereas the other were new compounds in this field. The new compounds were all organized around a bis-(heteroaryl)maleimides scaffold.
Bouchet, Nicolas, Bischerour, Julien, Germon, Stephanie, Guillard, Jerome, Dubernet, Mathieu, Viaud-Massuard, Maire-Claude, Delelis, Olivier, Ryabinin, Vladimir, Bigot, Yves, Auge-Gouillou, Corinne   +9 more
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Multicopy Chromosomal Integration Using CRISPR-Associated Transposases

ACS Synthetic Biology, 2020
Controlling the copy number of gene expression cassettes is an important strategy to engineer bacterial cells into high-efficiency biocatalysts. Current strategies mostly use plasmid vectors, but multicopy plasmids are often genetically unstable, and their copy numbers cannot be precisely controlled.
Yiwen Zhang, Xiaoman Sun, Qingzhuo Wang, Jiaqi Xu, Feng Dong, Siqi Yang, Jiawei Yang, Zixu Zhang, Yuan Qian, Jun Chen, Jiao Zhang, Yingmiao Liu, Rongsheng Tao, Yu Jiang, Junjie Yang, Sheng Yang   +15 more
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Overproduction and Purification of the Tn3 Transposase

The Journal of Biochemistry, 1987
The Tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing Escherichia coli strain harboring a transposase overproducer plasmid. This accumulation of the transposase seems to be due to four factors: derepression of transcription resulting from inactivation of the repressor gene (tnpR); efficient translation caused by a ...
M, Morita, S, Tsunasawa, Y, Sugino
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Repression of the Ac‐transposase gene promoter by Ac transposase

The Plant Journal, 1996
SummaryMobility of the maize Ac‐Ds transposable element family depends on the production of Ac‐encoded transposase (TPASE). The TPASE is a DNA‐binding protein which recognizes internal sites near both Ac termini in a region which overlaps the putative TPASE gene promoter. Therefore, it was hypothesized that TPASE may regulate its own transcription. The
Marcelo Fridlender, Kate Harrison, Jonathan D.G. Jones, Avraham A. Levy   +3 more
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Mariner Mos1 transposase optimization by rational mutagenesis

Genetica, 2009
Mariner transposons are probably the most widespread transposable element family in animal genomes. To date, they are believed not to require species-specific host factors for transposition. Despite this, Mos1, one of the most-studied mariner elements (with Himar1), has been shown to be active in insects, but inactive in mammalian genomes.
Germon, Stephanie, Bouchet, Nicolas, Casteret, Sophie, Carpentier, Guillaume, Adet, Jeremy, Bigot, Yves, Auge-Gouillou, Corinne   +6 more
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Designing and Testing Chimeric Zinc Finger Transposases

2010
Transposons have been effectively utilized as non-viral gene delivery systems that are capable of promoting stable transgene expression in mammalian and human cells. Two specific transposon systems, Sleeping Beauty (SB) and piggyBac (PB), have been successfully modified by the addition of a zinc finger (ZF) DNA-binding domain as a strategy for ...
Matthew H, Wilson, Alfred L, George
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Molecular architecture of a eukaryotic DNA transposase

Nature Structural & Molecular Biology, 2005
Mobile elements and their inactive remnants account for large proportions of most eukaryotic genomes, where they have had central roles in genome evolution. Over 50 years ago, McClintock reported a form of stress-induced genome instability in maize in which discrete DNA segments move between chromosomal locations.
Alison B, Hickman, Zhanita N, Perez, Liqin, Zhou, Primrose, Musingarimi, Rodolfo, Ghirlando, Jenny E, Hinshaw, Nancy L, Craig, Fred, Dyda   +7 more
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