Results 51 to 60 of about 16,112 (276)

Transcriptome analysis of bacteriophage communities in periodontal health and disease. [PDF]

, 2015
BackgroundThe role of viruses as members of the human microbiome has gained broader attention with the discovery that human body surfaces are inhabited by sizeable viral communities.
Abeles, Shira R, Boehm, Tobias K, Ly, Melissa, Naidu, Mayuri, Pride, David T, Santiago-Rodriguez, Tasha M   +5 more
core   +2 more sources

Poly(lactic‐co‐glycolic acid) Nanoparticles for IL‐12 Self‐Amplifying RNA Delivery in Glioblastoma Models

Advanced Functional Materials, EarlyView.
xxxx. ABSTRACT Glioblastoma (GBM) remains one of the most lethal brain cancers, with median survival rarely exceeding 15 months after diagnosis. Interleukin‐12 (IL‐12) is a potent immunostimulatory cytokine capable of reshaping the tumor microenvironment (TME), yet its clinical translation is hindered by systemic toxicity and short half‐life. RNA‐based
Fatima Hameedat, Johan Sebastian Lopez‐Parra, Willem M. H. Hoogaars, Emma Dijkstra, Cynthia Huang, Irafasha C. Casmil, Anna K. Blakney, Peter Olinga, Barbara Rothen‐Rutishauser, Hélder A. Santos, Alke Petri‐Fink, Flávia Sousa   +11 more
wiley   +1 more source

Sequence diversity and copy number variation of Mutator-like transposases in wheat

Genetics and Molecular Biology, 2008
Partial transposase-coding sequences of Mutator-like elements (MULEs) were isolated from a wild einkorn wheat, Triticum urartu, by degenerate PCR.
Nobuaki Asakura, Shinya Yoshida, Naoki Mori, Ichiro Ohtsuka, Chiharu Nakamura   +4 more
doaj   +1 more source

Diverse Systems for Efficient Sequence Insertion and Replacement in Precise Plant Genome Editing

BioDesign Research, 2020
CRISPR-mediated genome editing has been widely applied in plants to make uncomplicated genomic modifications including gene knockout and base changes.
Yingxiao Zhang, Yiping Qi
doaj   +1 more source

Draft Genome Sequence of Mycobacterium heckeshornense Strain RLE. [PDF]

, 2015
We report here the draft genome sequence of Mycobacterium heckeshornense strain RLE isolated from a sputum sample from a patient with shortness of breath.
Chiu, Charles Y, Cunningham, Gail, Greninger, Alexander L, Miller, Steve   +3 more
core   +1 more source

Improved genome sequencing using an engineered transposase [PDF]

BMC Biotechnology, 2017
Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transposase-based Nextera method was invented. Nextera combines DNA fragmentation and barcoding in a single tube reaction and therefore enables a very ...
Kia, Amirali, Gloeckner, Christian, Osothprarop, Trina, Gormley, Niall, Bomati, Erin, Stephenson, Michelle, Goryshin, Igor, He, Molly Min   +7 more
openaire   +2 more sources

ETV2 Mediated Differentiation of Human Pluripotent Stem Cells Results in Functional Endothelial Cells for Engineering Advanced Vascularized Microphysiological Models

Advanced Healthcare Materials, EarlyView.
A robust method to generate functional human iPSC‐derived endothelial cells using inducible ETV2 expression. These cells self‐organize into stable, lumenized microvascular networks within microfluidic chips, surpassing conventional differentiation methods.
Shun Zhang, Zhengpeng Wan, Lei Wang, Caihong Wu, Junkai Zhang, Sarah Spitz, Xun Wang, Marie A. Floryan, Mark F. Coughlin, Francesca M. Pramotton, Liling Xu, Ron Weiss, Roger D. Kamm   +12 more
wiley   +1 more source

Strand-specific community RNA-seq reveals prevalent and dynamic antisense transcription in human gut microbiota

Frontiers in Microbiology, 2015
Metagenomics and other metaomics approaches (including metatranscriptomics) provide insights into the composition and function of microbial communities living in different environments or animal hosts.
Guanhui eBao, Mingjie eWang, Thomas G. Doak, Yuzhen eYe   +3 more
doaj   +1 more source

Nanopore sequencing reveals genomic map of CTX-M-type extended-spectrum β-lactamases carried by Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway

BMC Microbiology, 2020
Background Environmental surveillance of antibiotic resistance can contribute towards better understanding and management of human and environmental health.
Didrik H. Grevskott, Francisco Salvà-Serra, Edward R. B. Moore, Nachiket P. Marathe   +3 more
doaj   +1 more source

piggyBac transposase tools for genome engineering [PDF]

Proceedings of the National Academy of Sciences, 2013
Significance DNA transposons that translocate by excision from a donor site and insertion into a target site are often used for genome engineering by insertional mutagenesis and transgenesis. The piggyBac element is especially useful because it can excise precisely from an insertion site, restoring the ...
Xianghong, Li, Erin R, Burnight, Ashley L, Cooney, Nirav, Malani, Troy, Brady, Jeffry D, Sander, Janice, Staber, Sarah J, Wheelan, J Keith, Joung, Paul B, McCray, Frederic D, Bushman, Patrick L, Sinn, Nancy L, Craig   +12 more
openaire   +2 more sources