Results 51 to 60 of about 10,355 (255)

Nanopore sequencing reveals genomic map of CTX-M-type extended-spectrum β-lactamases carried by Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway

BMC Microbiology, 2020
Background Environmental surveillance of antibiotic resistance can contribute towards better understanding and management of human and environmental health.
Didrik H. Grevskott, Francisco Salvà-Serra, Edward R. B. Moore, Nachiket P. Marathe   +3 more
doaj   +1 more source

A hyperactive piggyBac transposase for mammalian applications [PDF]

Proceedings of the National Academy of Sciences, 2011
DNA transposons have been widely used for transgenesis and insertional mutagenesis in various organisms. Among the transposons active in mammalian cells, the moth-derived transposon piggyBac is most promising with its highly efficient transposition, large cargo capacity, and precise repair of
Yusa K, Zhou L, Li MA, Bradley A, Craig NL   +4 more
openaire   +2 more sources

Pharmacological chromatin remodeling enhances response to estrogen therapy in ER+ breast cancer

Molecular Oncology, EarlyView.
Estrogen therapy elicits clinical benefit in ~ 30% of patients with endocrine‐resistant estrogen receptor (ER)‐positive breast cancer. Based on findings that ER transcriptional activation underlies response to estrogen therapy, we tested the effects of epigenetic dysregulation via pharmacological inhibition of histone deacetylases (HDACi).
Anneka L. Johnson Thomas, Barbara Karakyriakou, Patricia C. Muskus, Alyssa M. Roberts, Justin Rendleman, Alexa Warren, Hanxu Lu, Kimberly A. Birmingham, Malone K. Friedman, Abigail E. Goen, Huijuan Yang, Owen M. Wilkins, Fred W. Kolling, Eugene Demidenko, Julie M. Jorns, Brock C. Christensen, Todd W. Miller   +16 more
wiley   +1 more source

Strand-specific community RNA-seq reveals prevalent and dynamic antisense transcription in human gut microbiota

Frontiers in Microbiology, 2015
Metagenomics and other metaomics approaches (including metatranscriptomics) provide insights into the composition and function of microbial communities living in different environments or animal hosts.
Guanhui eBao, Mingjie eWang, Thomas G. Doak, Yuzhen eYe   +3 more
doaj   +1 more source

Functional characterization of an active Rag-like transposase [PDF]

Nature Structural & Molecular Biology, 2012
The formation of diverse immunoglobulin genes results in part from Rag protein-mediated DNA double-strand breaks at the edges of immunoglobulin gene segments, followed by combinatorial reassembly of these segments. We report that a Transib transposase from the insect Helicoverpa zea is active in vitro and that its breakage and joining activities mimic ...
Hencken, Cary G., Li, Xianghong, Craig, Nancy L.   +2 more
openaire   +2 more sources

Spatial biology in cancer epigenetics

Molecular Oncology, EarlyView.
Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley   +1 more source

Recurrent co-domestication of PIF/Harbinger transposable element proteins in insects

Mobile DNA, 2022
Background Transposable elements (TEs) are selfish DNA sequences capable of moving and amplifying at the expense of host cells. Despite this, an increasing number of studies have revealed that TE proteins are important contributors to the emergence of ...
Dragomira N. Markova, Fatema B. Ruma, Claudio Casola, Ayda Mirsalehi, Esther Betrán   +4 more
doaj   +1 more source

Genome‐Wide In Vivo RNAi Screening Identifies HOXD4 as a Tumor Metastasis Suppressor in Colorectal Cancer

Advanced Science, EarlyView.
Metastasis remains a major challenge in colorectal cancer. Using an in vivo shRNA screening system, this study identifies Homeobox D4 as a key metastasis suppressor. Reduced Homeobox D4 expression is associated with aggressive tumor features. Functional and mechanistic analyses show that it inhibits epithelial‐mesenchymal transition by repressing ...
Zhi‐hua Ye, Wen‐jing Luo, Lu Li, Wen‐di Shuai, Ling Zhou, You‐fa Duan, Xue Chen, Jun‐kai Zhang, Wenlin Huang, Ran‐yi Liu   +9 more
wiley   +1 more source

The domesticated transposase ALP2 mediates formation of a novel Polycomb protein complex by direct interaction with MSI1, a core subunit of Polycomb Repressive Complex 2 (PRC2).

PLoS Genetics, 2020
A large fraction of plant genomes is composed of transposable elements (TE), which provide a potential source of novel genes through "domestication"-the process whereby the proteins encoded by TE diverge in sequence, lose their ability to catalyse ...
Christos N Velanis, Pumi Perera, Bennett Thomson, Erica de Leau, Shih Chieh Liang, Ben Hartwig, Alexander Förderer, Harry Thornton, Pedro Arede, Jiawen Chen, Kimberly M Webb, Serin Gümüs, Geert De Jaeger, Clinton A Page, C Nathan Hancock, Christos Spanos, Juri Rappsilber, Philipp Voigt, Franziska Turck, Frank Wellmer, Justin Goodrich   +20 more
doaj   +1 more source

Tn 5 Transposase Active Site Mutants [PDF]

Journal of Biological Chemistry, 2002
Tn5 transposase (Tnp) is a 53.3-kDa protein that is encoded by and facilitates movement of transposon Tn5. Tnp monomers contain a single active site that is responsible for catalyzing a series of four DNA breaking/joining reactions at one transposon end.
Todd A, Naumann, William S, Reznikoff
openaire   +2 more sources