Machines on Genes: Enzymes that Make, Break and Move DNA and RNA [PDF]
, 2010 As the vital information repositories of the cell, the nucleic acids DNA and RNA pose many challenges as enzyme substrates. To produce, maintain and repair DNA and RNA, and to extract the genetic information that they encode, a battery of remarkable ...
Bates +13 more
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Structural Determinants of Sleeping Beauty Transposase Activity [PDF]
Molecular Therapy, 2016 Transposases are important tools in genome engineering, and there is considerable interest in engineering more efficient ones. Here, we seek to understand the factors determining their activity using the Sleeping Beauty transposase. Recent work suggests that protein coevolutionary information can be used to classify groups of physically connected ...
Abrusán, György +7 more
openaire +4 more sources
Zoological Letters, 2018 Background DNA transposons are generally destroyed by mutations and have short lifespans in hosts, as they are neutral or harmful to the host and therefore not conserved by natural selection.
Akira Hikosaka, Seigo Konishi
doaj +1 more source
Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion [PDF]
, 2018 In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited.
A Bolotin +76 more
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Isolation and characterization of Tn7 transposase gain-of-function mutants: a model for transposase activation [PDF]
The EMBO Journal, 2000 Tn7 transposition has been hypothesized to require a heteromeric transposase formed by two Tn7-encoded proteins, TnsA and TnsB, and accessory proteins that activate the transposase when they are associated with an appropriate target DNA. This study investigates the mechanism of Tn7 transposase activation by isolation and analysis of transposase gain-of-
F, Lu, N L, Craig
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Six domesticated PiggyBac transposases together carry out programmed DNA elimination in Paramecium
eLife, 2018 The domestication of transposable elements has repeatedly occurred during evolution and domesticated transposases have often been implicated in programmed genome rearrangements, as remarkably illustrated in ciliates.
Julien Bischerour +11 more
doaj +1 more source
How to kill the honey bee larva: genomic potential and virulence mechanisms of Paenibacillus larvae.
PLoS ONE, 2014 Paenibacillus larvae, a Gram positive bacterial pathogen, causes American Foulbrood (AFB), which is the most serious infectious disease of honey bees. In order to investigate the genomic potential of P.
Marvin Djukic +9 more
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Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. [PDF]
, 2013 Insertional therapies have shown great potential for combating genetic disease and safer methods would undoubtedly broaden the variety of possible illness that can be treated. A major challenge that remains is reducing the risk of insertional mutagenesis
Bhakta, Mital S +6 more
core +3 more sources
Next-generation sequencing library construction on a surface
BMC Genomics, 2018 Background Next-generation sequencing (NGS) has revolutionized almost all fields of biology, agriculture and medicine, and is widely utilized to analyse genetic variation.
Kuan Feng +2 more
doaj +1 more source
Systems Biology and Pangenome of Salmonella O-Antigens. [PDF]
, 2019 O-antigens are glycopolymers in lipopolysaccharides expressed on the cell surface of Gram-negative bacteria. Variability in the O-antigen structure constitutes the basis for the establishment of the serotyping schema. We pursued a two-pronged approach to
Kavvas, Erol +4 more
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